Condensed tannins in mangosteen pericarps determined from ultraperformance liquid chromatography-mass spectrometry
The objective of the present work was to determine the types and concentrations of condensed tannins in mangosteen pericarps. Methanol, ethanol, and acetone were employed to extract tannins from the mangosteen pericarps. The tannin extracts were depolymerized using 2-mercaptoethanol as a nucleophi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2020-02-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | https://rdo.psu.ac.th/sjstweb/journal/42-1/18.pdf |
| _version_ | 1818063928967561216 |
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| author | Narita Khundamri Chahinez Aouf Hélène Fulcrand Eric Dubreucq Varaporn Tanrattanakul |
| author_facet | Narita Khundamri Chahinez Aouf Hélène Fulcrand Eric Dubreucq Varaporn Tanrattanakul |
| author_sort | Narita Khundamri |
| collection | DOAJ |
| description | The objective of the present work was to determine the types and concentrations of condensed tannins in mangosteen
pericarps. Methanol, ethanol, and acetone were employed to extract tannins from the mangosteen pericarps. The tannin extracts
were depolymerized using 2-mercaptoethanol as a nucleophile. Ultra-performance liquid chromatography-mass spectrometry
(UPLC-MS) was used to structurally identify the tannins and determine their concentrations. UPLC chromatograms showed five
different tannin monomers: catechin, epicatechin, catechin mercapto, catechin gallate mercapto, and epigallocatechin gallate.
Catechin mercapto was the major monomer (~32 to 60 mg/g of extract). The average degree of polymerization indicated that the
condensed tannins in the pericarps were mainly dimers and trimers. The experimental results showed that methanol was the most
efficient extraction solvent in the present work and that UPLC-MS was a powerful technique for evaluating the condensed
tannins in mangosteen pericarps in terms of qualitative and quantitative analysis. |
| first_indexed | 2024-12-10T14:27:54Z |
| format | Article |
| id | doaj.art-151b4d6ed56f4efb83432bf318749dd6 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-10T14:27:54Z |
| publishDate | 2020-02-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-151b4d6ed56f4efb83432bf318749dd62022-12-22T01:45:01ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952020-02-0142113213810.14456/sjst-psu.2020.18Condensed tannins in mangosteen pericarps determined from ultraperformance liquid chromatography-mass spectrometryNarita Khundamri0Chahinez Aouf1Hélène Fulcrand2Eric Dubreucq3Varaporn Tanrattanakul4Department of Materials Science and Technology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110 ThailandINRA, UMR1083 Sciences Pour l’Oenologie, Université de Montpellier, Montpellier, 34060 FranceINRA, UMR1083 Sciences Pour l’Oenologie, Université de Montpellier, Montpellier, 34060 FranceMontpellier SupAgro, UMR1208 IATE, Université de Montpellier, Montpellier Cedex, 34060 FranceDepartment of Materials Science and Technology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110 ThailandThe objective of the present work was to determine the types and concentrations of condensed tannins in mangosteen pericarps. Methanol, ethanol, and acetone were employed to extract tannins from the mangosteen pericarps. The tannin extracts were depolymerized using 2-mercaptoethanol as a nucleophile. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) was used to structurally identify the tannins and determine their concentrations. UPLC chromatograms showed five different tannin monomers: catechin, epicatechin, catechin mercapto, catechin gallate mercapto, and epigallocatechin gallate. Catechin mercapto was the major monomer (~32 to 60 mg/g of extract). The average degree of polymerization indicated that the condensed tannins in the pericarps were mainly dimers and trimers. The experimental results showed that methanol was the most efficient extraction solvent in the present work and that UPLC-MS was a powerful technique for evaluating the condensed tannins in mangosteen pericarps in terms of qualitative and quantitative analysis.https://rdo.psu.ac.th/sjstweb/journal/42-1/18.pdfcondensed tannindepolymerizationsolvent extractionmangosteen pericarpsuplc-ms |
| spellingShingle | Narita Khundamri Chahinez Aouf Hélène Fulcrand Eric Dubreucq Varaporn Tanrattanakul Condensed tannins in mangosteen pericarps determined from ultraperformance liquid chromatography-mass spectrometry Songklanakarin Journal of Science and Technology (SJST) condensed tannin depolymerization solvent extraction mangosteen pericarps uplc-ms |
| title | Condensed tannins in mangosteen pericarps determined from ultraperformance liquid chromatography-mass spectrometry |
| title_full | Condensed tannins in mangosteen pericarps determined from ultraperformance liquid chromatography-mass spectrometry |
| title_fullStr | Condensed tannins in mangosteen pericarps determined from ultraperformance liquid chromatography-mass spectrometry |
| title_full_unstemmed | Condensed tannins in mangosteen pericarps determined from ultraperformance liquid chromatography-mass spectrometry |
| title_short | Condensed tannins in mangosteen pericarps determined from ultraperformance liquid chromatography-mass spectrometry |
| title_sort | condensed tannins in mangosteen pericarps determined from ultraperformance liquid chromatography mass spectrometry |
| topic | condensed tannin depolymerization solvent extraction mangosteen pericarps uplc-ms |
| url | https://rdo.psu.ac.th/sjstweb/journal/42-1/18.pdf |
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