Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10
Many studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More accurate expression patterns of boar sperm proteins may be de...
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| Format: | Article |
| Language: | English |
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Korean Society of Animal Sciences and Technology
2023-03-01
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| Series: | Journal of Animal Science and Technology |
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| Online Access: | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2023.e27 |
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| author | Yong-Min Kim Sung-Woo Park Mi-Jin Lee Da-Yeon Jeon Su-Jin Sa Yong-Dae Jeong Ha-Seung Seong Jung-Woo Choi Shinichi Hochi Eun-Seok Cho Hak-Jae Chung |
| author_facet | Yong-Min Kim Sung-Woo Park Mi-Jin Lee Da-Yeon Jeon Su-Jin Sa Yong-Dae Jeong Ha-Seung Seong Jung-Woo Choi Shinichi Hochi Eun-Seok Cho Hak-Jae Chung |
| author_sort | Yong-Min Kim |
| collection | DOAJ |
| description | Many studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More accurate expression patterns of boar sperm proteins may be derived using the isobaric tags for relative and absolute quantification (iTRAQ) technique. In this study, the iTRAQ-labeling system was coupled with liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify differentially expressed CM10-fractionated proteins between fresh and frozen-thawed boar semen. A total of 76 protein types were identified to be differentially expressed, among which 9 and 67 proteins showed higher and lower expression in frozen-thawed than in fresh sperm samples, respectively. The classified functions of these proteins included oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes, which are involved in adenosine triphosphate (ATP) synthesis; and sperm flagellum and motile cilium, which are involved in sperm tail structure. These results suggest a possible network of biomarkers associated with survival after the cryopreservation of Duroc boar semen. |
| first_indexed | 2024-04-09T18:19:35Z |
| format | Article |
| id | doaj.art-151e2a1eb6da463dae578ccf1cfa091b |
| institution | Directory Open Access Journal |
| issn | 2672-0191 2055-0391 |
| language | English |
| last_indexed | 2024-04-09T18:19:35Z |
| publishDate | 2023-03-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-151e2a1eb6da463dae578ccf1cfa091b2023-04-12T08:25:44ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912023-03-0165240141110.5187/jast.2023.e27Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10Yong-Min Kim0Sung-Woo Park1Mi-Jin Lee2Da-Yeon Jeon3Su-Jin Sa4Yong-Dae Jeong5Ha-Seung Seong6Jung-Woo Choi7Shinichi Hochi8Eun-Seok Cho9Hak-Jae Chung10Planning and Coordination Division, National Institute of Animal Science, Wanju 55365, KoreaSwine Science Division, National Institute of Animal Science, Cheonan 31000, KoreaTNT Research, Doosan Venture Digm, Anyang 14059, KoreaSwine Science Division, National Institute of Animal Science, Cheonan 31000, KoreaSwine Science Division, National Institute of Animal Science, Cheonan 31000, KoreaSwine Science Division, National Institute of Animal Science, Cheonan 31000, KoreaSwine Science Division, National Institute of Animal Science, Cheonan 31000, KoreaDepartment of Animal Science, College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, KoreaFaculty of Textile Science and Technology, Shinshu University, Nagano 386-8567, JapanSwine Science Division, National Institute of Animal Science, Cheonan 31000, KoreaSwine Science Division, National Institute of Animal Science, Cheonan 31000, KoreaMany studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More accurate expression patterns of boar sperm proteins may be derived using the isobaric tags for relative and absolute quantification (iTRAQ) technique. In this study, the iTRAQ-labeling system was coupled with liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify differentially expressed CM10-fractionated proteins between fresh and frozen-thawed boar semen. A total of 76 protein types were identified to be differentially expressed, among which 9 and 67 proteins showed higher and lower expression in frozen-thawed than in fresh sperm samples, respectively. The classified functions of these proteins included oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes, which are involved in adenosine triphosphate (ATP) synthesis; and sperm flagellum and motile cilium, which are involved in sperm tail structure. These results suggest a possible network of biomarkers associated with survival after the cryopreservation of Duroc boar semen. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2023.e27Boar semenIsobaric tags for relative and absolute quantification (iTRAQ)ProteinChipSurface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS)Semen cryopreservation |
| spellingShingle | Yong-Min Kim Sung-Woo Park Mi-Jin Lee Da-Yeon Jeon Su-Jin Sa Yong-Dae Jeong Ha-Seung Seong Jung-Woo Choi Shinichi Hochi Eun-Seok Cho Hak-Jae Chung Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10 Journal of Animal Science and Technology Boar semen Isobaric tags for relative and absolute quantification (iTRAQ) ProteinChip Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) Semen cryopreservation |
| title | Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10 |
| title_full | Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10 |
| title_fullStr | Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10 |
| title_full_unstemmed | Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10 |
| title_short | Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10 |
| title_sort | profiling of differentially expressed proteins between fresh and frozen thawed duroc boar semen using proteinchip cm10 |
| topic | Boar semen Isobaric tags for relative and absolute quantification (iTRAQ) ProteinChip Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) Semen cryopreservation |
| url | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2023.e27 |
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