Reprogramming of one human induced pluripotent stem cell line from healthy donor
In this study, skin biopsy was, and the fibroblasts were isolated from the dermal explant cultures. Human induced pluripotent stem cell (iPSC) line was generated from the skin fibroblasts collected from a healthy 50-year old male donor with informed consent. The reprogramming of fibroblasts was perf...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506121004608 |
| _version_ | 1818459973187796992 |
|---|---|
| author | Shuxing Cao Jun Ma Jinyu Zhang Ruiyun Guo Xin Liu Ming Li |
| author_facet | Shuxing Cao Jun Ma Jinyu Zhang Ruiyun Guo Xin Liu Ming Li |
| author_sort | Shuxing Cao |
| collection | DOAJ |
| description | In this study, skin biopsy was, and the fibroblasts were isolated from the dermal explant cultures. Human induced pluripotent stem cell (iPSC) line was generated from the skin fibroblasts collected from a healthy 50-year old male donor with informed consent. The reprogramming of fibroblasts was performed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc. The generated iPSCs were confirmed integration-free, expressed pluripotency markers, displayed the normal karyotype, and demonstrated trilineage differentiation potential. This iPSC model can be used to model physiological processes and screen drug validation in vitro. |
| first_indexed | 2024-12-14T23:22:51Z |
| format | Article |
| id | doaj.art-152251b37c3a47b6a9a1ea70f32d6067 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-14T23:22:51Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-152251b37c3a47b6a9a1ea70f32d60672022-12-21T22:43:54ZengElsevierStem Cell Research1873-50612021-12-0157102613Reprogramming of one human induced pluripotent stem cell line from healthy donorShuxing Cao0Jun Ma1Jinyu Zhang2Ruiyun Guo3Xin Liu4Ming Li5The Second Hospital of Hebei Medical University, Hebei Province 050017, ChinaHebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, ChinaHebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaHebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaHebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, ChinaThe Second Hospital of Hebei Medical University, Hebei Province 050017, China; Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Corresponding author at: The Second Hospital of Hebei Medical University, Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China.In this study, skin biopsy was, and the fibroblasts were isolated from the dermal explant cultures. Human induced pluripotent stem cell (iPSC) line was generated from the skin fibroblasts collected from a healthy 50-year old male donor with informed consent. The reprogramming of fibroblasts was performed with four Yamanaka factors containing Oct3/4, Sox2, Klf4 and c-Myc. The generated iPSCs were confirmed integration-free, expressed pluripotency markers, displayed the normal karyotype, and demonstrated trilineage differentiation potential. This iPSC model can be used to model physiological processes and screen drug validation in vitro.http://www.sciencedirect.com/science/article/pii/S1873506121004608 |
| spellingShingle | Shuxing Cao Jun Ma Jinyu Zhang Ruiyun Guo Xin Liu Ming Li Reprogramming of one human induced pluripotent stem cell line from healthy donor Stem Cell Research |
| title | Reprogramming of one human induced pluripotent stem cell line from healthy donor |
| title_full | Reprogramming of one human induced pluripotent stem cell line from healthy donor |
| title_fullStr | Reprogramming of one human induced pluripotent stem cell line from healthy donor |
| title_full_unstemmed | Reprogramming of one human induced pluripotent stem cell line from healthy donor |
| title_short | Reprogramming of one human induced pluripotent stem cell line from healthy donor |
| title_sort | reprogramming of one human induced pluripotent stem cell line from healthy donor |
| url | http://www.sciencedirect.com/science/article/pii/S1873506121004608 |
| work_keys_str_mv | AT shuxingcao reprogrammingofonehumaninducedpluripotentstemcelllinefromhealthydonor AT junma reprogrammingofonehumaninducedpluripotentstemcelllinefromhealthydonor AT jinyuzhang reprogrammingofonehumaninducedpluripotentstemcelllinefromhealthydonor AT ruiyunguo reprogrammingofonehumaninducedpluripotentstemcelllinefromhealthydonor AT xinliu reprogrammingofonehumaninducedpluripotentstemcelllinefromhealthydonor AT mingli reprogrammingofonehumaninducedpluripotentstemcelllinefromhealthydonor |