Optimized Isolation and Characterization of C57BL/6 Mouse Hepatic Stellate Cells

To obtain meaningful results of hepatic stellate cell (HSC) function, it is crucial to use highly pure HSC populations. Our aim was to optimize HSC isolation from mice livers without exploiting the characteristically transient vitamin A autofluorescence of HSC. HSCs were isolated from C57BL/6 mice u...

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Bibliographic Details
Main Authors: Alexandre Balaphas, Jeremy Meyer, Cécile Gameiro, Aurélien Frobert, Marie-Noëlle Giraud, Bernhard Egger, Leo H. Bühler, Carmen Gonelle-Gispert
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:Cells
Subjects:
Online Access:https://www.mdpi.com/2073-4409/11/9/1379