Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting
Background: 3D bioprinting is the future of constructing functional organs. Creating a bioactive scaffold with pancreatic islets presents many challenges. The aim of this paper is to assess how the 3D bioprinting process affects islet viability. Methods: The BioX 3D printer (Cellink), 600 μm inner d...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Online Access: | https://www.mdpi.com/2072-666X/12/3/304 |
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| author | Marta Klak Patrycja Kowalska Tomasz Dobrzański Grzegorz Tymicki Piotr Cywoniuk Magdalena Gomółka Katarzyna Kosowska Tomasz Bryniarski Andrzej Berman Agnieszka Dobrzyń Wojciech Sadowski Bartosz Górecki Michał Wszoła |
| author_facet | Marta Klak Patrycja Kowalska Tomasz Dobrzański Grzegorz Tymicki Piotr Cywoniuk Magdalena Gomółka Katarzyna Kosowska Tomasz Bryniarski Andrzej Berman Agnieszka Dobrzyń Wojciech Sadowski Bartosz Górecki Michał Wszoła |
| author_sort | Marta Klak |
| collection | DOAJ |
| description | Background: 3D bioprinting is the future of constructing functional organs. Creating a bioactive scaffold with pancreatic islets presents many challenges. The aim of this paper is to assess how the 3D bioprinting process affects islet viability. Methods: The BioX 3D printer (Cellink), 600 μm inner diameter nozzles, and 3% (<i>w/v</i>) alginate cell carrier solution were used with rat, porcine, and human pancreatic islets. Islets were divided into a control group (culture medium) and 6 experimental groups (each subjected to specific pressure between 15 and 100 kPa). FDA/PI staining was performed to assess the viability of islets. Analogous studies were carried out on α-cells, β-cells, fibroblasts, and endothelial cells. Results: Viability of human pancreatic islets was as follows: 92% for alginate-based control and 94%, 90%, 74%, 48%, 61%, and 59% for 15, 25, 30, 50, 75, and 100 kPa, respectively. Statistically significant differences were observed between control and 50, 75, and 100 kPa, respectively. Similar observations were made for porcine and rat islets. Conclusions: Optimal pressure during 3D bioprinting with pancreatic islets by the extrusion method should be lower than 30 kPa while using 3% (<i>w/v</i>) alginate as a carrier. |
| first_indexed | 2024-03-10T13:14:11Z |
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| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T13:14:11Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-15249a7ea8bd454fba8a7d6609f36ea02023-11-21T10:29:08ZengMDPI AGMicromachines2072-666X2021-03-0112330410.3390/mi12030304Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D BioprintingMarta Klak0Patrycja Kowalska1Tomasz Dobrzański2Grzegorz Tymicki3Piotr Cywoniuk4Magdalena Gomółka5Katarzyna Kosowska6Tomasz Bryniarski7Andrzej Berman8Agnieszka Dobrzyń9Wojciech Sadowski10Bartosz Górecki11Michał Wszoła12Foundation of Research and Science Development, 01-793 Warsaw, PolandFoundation of Research and Science Development, 01-793 Warsaw, PolandFoundation of Research and Science Development, 01-793 Warsaw, PolandFoundation of Research and Science Development, 01-793 Warsaw, PolandFoundation of Research and Science Development, 01-793 Warsaw, PolandFoundation of Research and Science Development, 01-793 Warsaw, PolandFoundation of Research and Science Development, 01-793 Warsaw, PolandFoundation of Research and Science Development, 01-793 Warsaw, PolandFoundation of Research and Science Development, 01-793 Warsaw, PolandNencki Institute of Experimental Biology, Polish Academy of Sciences, 02-093 Warsaw, PolandQuickerSim Sp. z o.o., 00-666 Warsaw, PolandQuickerSim Sp. z o.o., 00-666 Warsaw, PolandFoundation of Research and Science Development, 01-793 Warsaw, PolandBackground: 3D bioprinting is the future of constructing functional organs. Creating a bioactive scaffold with pancreatic islets presents many challenges. The aim of this paper is to assess how the 3D bioprinting process affects islet viability. Methods: The BioX 3D printer (Cellink), 600 μm inner diameter nozzles, and 3% (<i>w/v</i>) alginate cell carrier solution were used with rat, porcine, and human pancreatic islets. Islets were divided into a control group (culture medium) and 6 experimental groups (each subjected to specific pressure between 15 and 100 kPa). FDA/PI staining was performed to assess the viability of islets. Analogous studies were carried out on α-cells, β-cells, fibroblasts, and endothelial cells. Results: Viability of human pancreatic islets was as follows: 92% for alginate-based control and 94%, 90%, 74%, 48%, 61%, and 59% for 15, 25, 30, 50, 75, and 100 kPa, respectively. Statistically significant differences were observed between control and 50, 75, and 100 kPa, respectively. Similar observations were made for porcine and rat islets. Conclusions: Optimal pressure during 3D bioprinting with pancreatic islets by the extrusion method should be lower than 30 kPa while using 3% (<i>w/v</i>) alginate as a carrier.https://www.mdpi.com/2072-666X/12/3/304bioprinting 3Dshear forcesviabilitycellsislets |
| spellingShingle | Marta Klak Patrycja Kowalska Tomasz Dobrzański Grzegorz Tymicki Piotr Cywoniuk Magdalena Gomółka Katarzyna Kosowska Tomasz Bryniarski Andrzej Berman Agnieszka Dobrzyń Wojciech Sadowski Bartosz Górecki Michał Wszoła Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting Micromachines bioprinting 3D shear forces viability cells islets |
| title | Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
| title_full | Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
| title_fullStr | Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
| title_full_unstemmed | Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
| title_short | Bionic Organs: Shear Forces Reduce Pancreatic Islet and Mammalian Cell Viability during the Process of 3D Bioprinting |
| title_sort | bionic organs shear forces reduce pancreatic islet and mammalian cell viability during the process of 3d bioprinting |
| topic | bioprinting 3D shear forces viability cells islets |
| url | https://www.mdpi.com/2072-666X/12/3/304 |
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