Expression of Chitinase and shRNA Gene Exhibits Resistance to Fungi and Virus
With the increasing global population, saving crops from diseases caused by different kinds of bacteria, fungi, viruses, and nematodes is essential. Potato is affected by various diseases, destroying many crops in the field and storage. In this study, we developed potato lines resistant to fungi and...
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MDPI AG
2023-05-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/5/1090 |
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| author | Samia Parveen Anwar Khan Nusrat Jahan Khadija Aaliya Adnan Muzaffar Bushra Tabassum Syed Inayatullah Syed Moeezullah Muhammad Tariq Zainia Rehmat Niaz Ali Abrar Hussain |
| author_facet | Samia Parveen Anwar Khan Nusrat Jahan Khadija Aaliya Adnan Muzaffar Bushra Tabassum Syed Inayatullah Syed Moeezullah Muhammad Tariq Zainia Rehmat Niaz Ali Abrar Hussain |
| author_sort | Samia Parveen |
| collection | DOAJ |
| description | With the increasing global population, saving crops from diseases caused by different kinds of bacteria, fungi, viruses, and nematodes is essential. Potato is affected by various diseases, destroying many crops in the field and storage. In this study, we developed potato lines resistant to fungi and viruses, Potato Virus X (PVX) and Potato Virus Y (PVY), by inoculating chitinase for fungi and shRNA designed against the mRNA of the coat protein of PVX and PVY, respectively. The construct was developed using the pCAMBIA2301 vector and transformed into AGB-R (red skin) potato cultivar using <i>Agrobacterium tumefaciens</i>. The crude protein extract of the transgenic potato plant inhibited the growth of <i>Fusarium oxysporum</i> from ~13 to 63%. The detached leaf assay of the transgenic line (SP-21) showed decreased necrotic spots compared to the non-transgenic control when challenged with <i>Fusarium oxysporum</i>. The transgenic line, SP-21, showed maximum knockdown when challenged with PVX and PVY, i.e., 89 and 86%, while transgenic line SP-148 showed 68 and 70% knockdown in the PVX- and PVY-challenged conditions, respectively. It is concluded from this study that the developed transgenic potato cultivar AGB-R showed resistance against fungi and viruses (PVX and PVY). |
| first_indexed | 2024-03-11T03:42:12Z |
| format | Article |
| id | doaj.art-15249c5f240047b3a032a5128fd46634 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T03:42:12Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-15249c5f240047b3a032a5128fd466342023-11-18T01:30:20ZengMDPI AGGenes2073-44252023-05-01145109010.3390/genes14051090Expression of Chitinase and shRNA Gene Exhibits Resistance to Fungi and VirusSamia Parveen0Anwar Khan1Nusrat Jahan2Khadija Aaliya3Adnan Muzaffar4Bushra Tabassum5Syed Inayatullah6Syed Moeezullah7Muhammad Tariq8Zainia Rehmat9Niaz Ali10Abrar Hussain11Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta 87300, PakistanDepartment of Microbiology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta 87300, PakistanDepartment of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta 87300, PakistanCenter of Excellence in Molecular Biology, University of the Punjab, Lahore 54000, PakistanCenter of Excellence in Molecular Biology, University of the Punjab, Lahore 54000, PakistanSchool of Biological Sciences, University of the Punjab, Lahore 54000, PakistanDepartment of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta 87300, PakistanDepartment of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta 87300, PakistanCenter of Excellence in Molecular Biology, University of the Punjab, Lahore 54000, PakistanDepartment of Biotechnology, Sardar Bahadur Khan Women’s University Balochistan, Quetta 87300, PakistanDepartment of Botany, Hazara University, Mansehra 21300, PakistanDepartment of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta 87300, PakistanWith the increasing global population, saving crops from diseases caused by different kinds of bacteria, fungi, viruses, and nematodes is essential. Potato is affected by various diseases, destroying many crops in the field and storage. In this study, we developed potato lines resistant to fungi and viruses, Potato Virus X (PVX) and Potato Virus Y (PVY), by inoculating chitinase for fungi and shRNA designed against the mRNA of the coat protein of PVX and PVY, respectively. The construct was developed using the pCAMBIA2301 vector and transformed into AGB-R (red skin) potato cultivar using <i>Agrobacterium tumefaciens</i>. The crude protein extract of the transgenic potato plant inhibited the growth of <i>Fusarium oxysporum</i> from ~13 to 63%. The detached leaf assay of the transgenic line (SP-21) showed decreased necrotic spots compared to the non-transgenic control when challenged with <i>Fusarium oxysporum</i>. The transgenic line, SP-21, showed maximum knockdown when challenged with PVX and PVY, i.e., 89 and 86%, while transgenic line SP-148 showed 68 and 70% knockdown in the PVX- and PVY-challenged conditions, respectively. It is concluded from this study that the developed transgenic potato cultivar AGB-R showed resistance against fungi and viruses (PVX and PVY).https://www.mdpi.com/2073-4425/14/5/1090<i>Fusarium oxysporum</i>shRNAvirus |
| spellingShingle | Samia Parveen Anwar Khan Nusrat Jahan Khadija Aaliya Adnan Muzaffar Bushra Tabassum Syed Inayatullah Syed Moeezullah Muhammad Tariq Zainia Rehmat Niaz Ali Abrar Hussain Expression of Chitinase and shRNA Gene Exhibits Resistance to Fungi and Virus Genes <i>Fusarium oxysporum</i> shRNA virus |
| title | Expression of Chitinase and shRNA Gene Exhibits Resistance to Fungi and Virus |
| title_full | Expression of Chitinase and shRNA Gene Exhibits Resistance to Fungi and Virus |
| title_fullStr | Expression of Chitinase and shRNA Gene Exhibits Resistance to Fungi and Virus |
| title_full_unstemmed | Expression of Chitinase and shRNA Gene Exhibits Resistance to Fungi and Virus |
| title_short | Expression of Chitinase and shRNA Gene Exhibits Resistance to Fungi and Virus |
| title_sort | expression of chitinase and shrna gene exhibits resistance to fungi and virus |
| topic | <i>Fusarium oxysporum</i> shRNA virus |
| url | https://www.mdpi.com/2073-4425/14/5/1090 |
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