Major Histocompatibility Complex-B haplotype and ovarian graft response
ABSTRACT: Gonadal tissue transfer is considered one of the best methods to preserve genetic variability. Poultry hosts can receive a gonad from a donor of a different genetic background, sustain the growth of this graft, and produce gametes from it. Unfortunately, the host's strong immune respo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123003693 |
| _version_ | 1797738977405435904 |
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| author | Chi Cuong Quach Janet E. Fulton James D. Benson Pamela Walker Crissandra Auckland Carl Lessard |
| author_facet | Chi Cuong Quach Janet E. Fulton James D. Benson Pamela Walker Crissandra Auckland Carl Lessard |
| author_sort | Chi Cuong Quach |
| collection | DOAJ |
| description | ABSTRACT: Gonadal tissue transfer is considered one of the best methods to preserve genetic variability. Poultry hosts can receive a gonad from a donor of a different genetic background, sustain the growth of this graft, and produce gametes from it. Unfortunately, the host's strong immune response may significantly reduce the gonadal graft's ability to reach maturity. Our study aimed to evaluate the influence of MHC-B alleles in rejecting a gonadal graft of similar or different genetic backgrounds. In the first experiment, ovarian tissue was transplanted to chicks of similar genetic backgrounds, either Lohmann White (LW) with variable MHC-B or Barred Rock (BR) with fixed MHC-B. The sustained growth of donor ovarian tissues occurred in (4/7 hosts) BR (MHC-B matched) hosts only—one of these graft-positive-BR hens produced eggs derived from the donor ovary. No grafts were recovered when the host and the donor had an LW background (0/9; MHC-B mismatched). In the second experiment, ovarian transplantation was done between chicks of either similar or different genetic backgrounds (Brown Leghorn [BL], BR, and BL/BR F1). The 2 pure lines contained only one MHC-B allele, whereas the F1 heterozygotes had both. All host birds were given a daily dose of an immunosuppressant (mycophenolate mofetil) until maturity. The success rate was assessed by microsatellite genotype confirmation of donor-derived ovaries plus physiological and histological analyses of ovarian grafts. In this second experiment, 11 out of 43 ovarian hosts laid eggs. However, all fertilized eggs from these hens were derived from the remnant host ovarian tissue, not from the donor ovaries. A necropsy assessment was done on all 43 host birds. Ten donor grafts were recovered from hosts having matched (6 hosts) and mismatched (4 hosts) MHC-B, and none were functional. Interestingly, 6 of them were enclosed by a serous membrane capsule filled with fluid and had various tissue growth. In addition, clusters of immune cells were observed in all recovered donor grafts. Our results demonstrated that genetic background could greatly influence the success of gonadal transfer in chickens. |
| first_indexed | 2024-03-12T13:50:40Z |
| format | Article |
| id | doaj.art-1527f13e323b49a8acc94333b3f87e7c |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-12T13:50:40Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-1527f13e323b49a8acc94333b3f87e7c2023-08-23T04:32:34ZengElsevierPoultry Science0032-57912023-09-011029102850Major Histocompatibility Complex-B haplotype and ovarian graft responseChi Cuong Quach0Janet E. Fulton1James D. Benson2Pamela Walker3Crissandra Auckland4Carl Lessard5Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, S7N 5B4, Canada; Agriculture and Agri-Food Canada, Saskatoon, SK, S7N 0×2, CanadaHy-Line International, Dallas Center, IA, 50063, USADepartment of Biology, College of Art and Science, University of Saskatchewan, Saskatoon, SK, S7N 5A5, CanadaAgriculture and Agri-Food Canada, Saskatoon, SK, S7N 0×2, CanadaAgriculture and Agri-Food Canada, Saskatoon, SK, S7N 0×2, CanadaAgriculture and Agri-Food Canada, Saskatoon, SK, S7N 0×2, Canada; Corresponding author:ABSTRACT: Gonadal tissue transfer is considered one of the best methods to preserve genetic variability. Poultry hosts can receive a gonad from a donor of a different genetic background, sustain the growth of this graft, and produce gametes from it. Unfortunately, the host's strong immune response may significantly reduce the gonadal graft's ability to reach maturity. Our study aimed to evaluate the influence of MHC-B alleles in rejecting a gonadal graft of similar or different genetic backgrounds. In the first experiment, ovarian tissue was transplanted to chicks of similar genetic backgrounds, either Lohmann White (LW) with variable MHC-B or Barred Rock (BR) with fixed MHC-B. The sustained growth of donor ovarian tissues occurred in (4/7 hosts) BR (MHC-B matched) hosts only—one of these graft-positive-BR hens produced eggs derived from the donor ovary. No grafts were recovered when the host and the donor had an LW background (0/9; MHC-B mismatched). In the second experiment, ovarian transplantation was done between chicks of either similar or different genetic backgrounds (Brown Leghorn [BL], BR, and BL/BR F1). The 2 pure lines contained only one MHC-B allele, whereas the F1 heterozygotes had both. All host birds were given a daily dose of an immunosuppressant (mycophenolate mofetil) until maturity. The success rate was assessed by microsatellite genotype confirmation of donor-derived ovaries plus physiological and histological analyses of ovarian grafts. In this second experiment, 11 out of 43 ovarian hosts laid eggs. However, all fertilized eggs from these hens were derived from the remnant host ovarian tissue, not from the donor ovaries. A necropsy assessment was done on all 43 host birds. Ten donor grafts were recovered from hosts having matched (6 hosts) and mismatched (4 hosts) MHC-B, and none were functional. Interestingly, 6 of them were enclosed by a serous membrane capsule filled with fluid and had various tissue growth. In addition, clusters of immune cells were observed in all recovered donor grafts. Our results demonstrated that genetic background could greatly influence the success of gonadal transfer in chickens.http://www.sciencedirect.com/science/article/pii/S0032579123003693immunosuppressionMHC-Bovarygonadal transfervitrification |
| spellingShingle | Chi Cuong Quach Janet E. Fulton James D. Benson Pamela Walker Crissandra Auckland Carl Lessard Major Histocompatibility Complex-B haplotype and ovarian graft response Poultry Science immunosuppression MHC-B ovary gonadal transfer vitrification |
| title | Major Histocompatibility Complex-B haplotype and ovarian graft response |
| title_full | Major Histocompatibility Complex-B haplotype and ovarian graft response |
| title_fullStr | Major Histocompatibility Complex-B haplotype and ovarian graft response |
| title_full_unstemmed | Major Histocompatibility Complex-B haplotype and ovarian graft response |
| title_short | Major Histocompatibility Complex-B haplotype and ovarian graft response |
| title_sort | major histocompatibility complex b haplotype and ovarian graft response |
| topic | immunosuppression MHC-B ovary gonadal transfer vitrification |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123003693 |
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