Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress
Water deficit is one of the main limiting factors for apple growth and production. Root architecture plays an important role in drought tolerance of plants. However, little is known about the molecular basis of root system in apple trees under drought. In this study, we compared root morphology of t...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-06-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311919625712 |
| _version_ | 1818642654325374976 |
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| author | Da-li GENG Li-yuan LU Ming-jia YAN Xiao-xia SHEN Li-juan JIANG Hai-yan LI Li-ping WANG Yan YAN Ji-di XU Cui-ying LI Jian-tao YU Feng-wang MA Qing-mei GUAN |
| author_facet | Da-li GENG Li-yuan LU Ming-jia YAN Xiao-xia SHEN Li-juan JIANG Hai-yan LI Li-ping WANG Yan YAN Ji-di XU Cui-ying LI Jian-tao YU Feng-wang MA Qing-mei GUAN |
| author_sort | Da-li GENG |
| collection | DOAJ |
| description | Water deficit is one of the main limiting factors for apple growth and production. Root architecture plays an important role in drought tolerance of plants. However, little is known about the molecular basis of root system in apple trees under drought. In this study, we compared root morphology of two widely used rootstocks of apple (R3 and Malus sieversii) under drought. Our results suggested that M. sieversii is more tolerant to drought than R3, since M. sieversii had a higher ratio of root to shoot as well as root hydraulic conductivity under long-term drought conditions. We then performed whole-genome transcriptomic analysis to figure out the molecular basis of root responses in M. sieversii under drought. It was found that genes involved in transcription regulation, signaling or biosynthesis of hormones, and oxidative stress were differentially expressed under drought. Consistent with the gene expression profile, roots of M. sieversii had higher activities of peroxidase (POD) and superoxide dismutase (SOD) under drought, as well as higher content of abscisic acid (ABA) and lower content of auxin. Taken together, our results revealed the physiological and transcriptomic analyses of M. sieversii roots in response to drought. |
| first_indexed | 2024-12-16T23:46:30Z |
| format | Article |
| id | doaj.art-152c4c6e473c4b5f9edbe9464da25eae |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-16T23:46:30Z |
| publishDate | 2019-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-152c4c6e473c4b5f9edbe9464da25eae2022-12-21T22:11:28ZengElsevierJournal of Integrative Agriculture2095-31192019-06-0118612801294Physiological and transcriptomic analyses of roots from Malus sieversii under drought stressDa-li GENG0Li-yuan LU1Ming-jia YAN2Xiao-xia SHEN3Li-juan JIANG4Hai-yan LI5Li-ping WANG6Yan YAN7Ji-di XU8Cui-ying LI9Jian-tao YU10Feng-wang MA11Qing-mei GUAN12State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. ChinaCollege of Information Engineering, Northwest A&F University, Yangling 712100, P.R. ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. China; Correspondence MA Feng-wang, Tel/Fax: +86-29-87082648State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, P.R. China; Correspondence GUAN Qing-mei, Tel/Fax: +86-29-87082648Water deficit is one of the main limiting factors for apple growth and production. Root architecture plays an important role in drought tolerance of plants. However, little is known about the molecular basis of root system in apple trees under drought. In this study, we compared root morphology of two widely used rootstocks of apple (R3 and Malus sieversii) under drought. Our results suggested that M. sieversii is more tolerant to drought than R3, since M. sieversii had a higher ratio of root to shoot as well as root hydraulic conductivity under long-term drought conditions. We then performed whole-genome transcriptomic analysis to figure out the molecular basis of root responses in M. sieversii under drought. It was found that genes involved in transcription regulation, signaling or biosynthesis of hormones, and oxidative stress were differentially expressed under drought. Consistent with the gene expression profile, roots of M. sieversii had higher activities of peroxidase (POD) and superoxide dismutase (SOD) under drought, as well as higher content of abscisic acid (ABA) and lower content of auxin. Taken together, our results revealed the physiological and transcriptomic analyses of M. sieversii roots in response to drought.http://www.sciencedirect.com/science/article/pii/S2095311919625712Malus sieversiiroot architecturedrought stressRNA-seq |
| spellingShingle | Da-li GENG Li-yuan LU Ming-jia YAN Xiao-xia SHEN Li-juan JIANG Hai-yan LI Li-ping WANG Yan YAN Ji-di XU Cui-ying LI Jian-tao YU Feng-wang MA Qing-mei GUAN Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress Journal of Integrative Agriculture Malus sieversii root architecture drought stress RNA-seq |
| title | Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress |
| title_full | Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress |
| title_fullStr | Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress |
| title_full_unstemmed | Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress |
| title_short | Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress |
| title_sort | physiological and transcriptomic analyses of roots from malus sieversii under drought stress |
| topic | Malus sieversii root architecture drought stress RNA-seq |
| url | http://www.sciencedirect.com/science/article/pii/S2095311919625712 |
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