Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span.
Vitamin K is a fat-soluble vitamin that plays important roles in blood coagulation and bone metabolism. One of its functions is as a co-factor for γ-glutamyl carboxylase (Ggcx). Conventional knockout of Ggcx causes death shortly after birth in homozygous mice. We created Ggcx-floxed mice by insertin...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3919827?pdf=render |
| _version_ | 1819132648366276608 |
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| author | Kotaro Azuma Tohru Tsukui Kazuhiro Ikeda Sachiko Shiba Kimie Nakagawa Toshio Okano Tomohiko Urano Kuniko Horie-Inoue Yasuyoshi Ouchi Masahito Ikawa Satoshi Inoue |
| author_facet | Kotaro Azuma Tohru Tsukui Kazuhiro Ikeda Sachiko Shiba Kimie Nakagawa Toshio Okano Tomohiko Urano Kuniko Horie-Inoue Yasuyoshi Ouchi Masahito Ikawa Satoshi Inoue |
| author_sort | Kotaro Azuma |
| collection | DOAJ |
| description | Vitamin K is a fat-soluble vitamin that plays important roles in blood coagulation and bone metabolism. One of its functions is as a co-factor for γ-glutamyl carboxylase (Ggcx). Conventional knockout of Ggcx causes death shortly after birth in homozygous mice. We created Ggcx-floxed mice by inserting loxP sequences at the sites flanking exon 6 of Ggcx. By mating these mice with albumin-Cre mice, we generated Ggcx-deficient mice specifically in hepatocytes (Ggcx(Δliver/Δliver) mice). In contrast to conventional Ggcx knockout mice, Ggcx(Δliver/Δliver) mice had very low activity of Ggcx in the liver and survived several weeks after birth. Furthermore, compared with heterozygous mice (Ggcx(+/Δliver) ), Ggcx(Δliver/Δliver) mice had shorter life spans. Ggcx(Δliver/Δliver) mice displayed bleeding diathesis, which was accompanied by decreased activity of coagulation factors II and IX. Ggcx-floxed mice can prove useful in examining Ggcx functions in vivo. |
| first_indexed | 2024-12-22T09:34:44Z |
| format | Article |
| id | doaj.art-152e93498b224cff9a184058adbf5bdb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T09:34:44Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-152e93498b224cff9a184058adbf5bdb2022-12-21T18:30:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8864310.1371/journal.pone.0088643Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span.Kotaro AzumaTohru TsukuiKazuhiro IkedaSachiko ShibaKimie NakagawaToshio OkanoTomohiko UranoKuniko Horie-InoueYasuyoshi OuchiMasahito IkawaSatoshi InoueVitamin K is a fat-soluble vitamin that plays important roles in blood coagulation and bone metabolism. One of its functions is as a co-factor for γ-glutamyl carboxylase (Ggcx). Conventional knockout of Ggcx causes death shortly after birth in homozygous mice. We created Ggcx-floxed mice by inserting loxP sequences at the sites flanking exon 6 of Ggcx. By mating these mice with albumin-Cre mice, we generated Ggcx-deficient mice specifically in hepatocytes (Ggcx(Δliver/Δliver) mice). In contrast to conventional Ggcx knockout mice, Ggcx(Δliver/Δliver) mice had very low activity of Ggcx in the liver and survived several weeks after birth. Furthermore, compared with heterozygous mice (Ggcx(+/Δliver) ), Ggcx(Δliver/Δliver) mice had shorter life spans. Ggcx(Δliver/Δliver) mice displayed bleeding diathesis, which was accompanied by decreased activity of coagulation factors II and IX. Ggcx-floxed mice can prove useful in examining Ggcx functions in vivo.http://europepmc.org/articles/PMC3919827?pdf=render |
| spellingShingle | Kotaro Azuma Tohru Tsukui Kazuhiro Ikeda Sachiko Shiba Kimie Nakagawa Toshio Okano Tomohiko Urano Kuniko Horie-Inoue Yasuyoshi Ouchi Masahito Ikawa Satoshi Inoue Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. PLoS ONE |
| title | Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
| title_full | Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
| title_fullStr | Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
| title_full_unstemmed | Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
| title_short | Liver-specific γ-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. |
| title_sort | liver specific γ glutamyl carboxylase deficient mice display bleeding diathesis and short life span |
| url | http://europepmc.org/articles/PMC3919827?pdf=render |
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