Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration
Abstract Improved materials for peripheral nerve repair are needed for the advancement of new surgical techniques in fields spanning from oncology to trauma. In this study, we developed bioresorbable materials capable of producing repeated electric field gradients spaced 600 μm apart to assess the i...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Springer
2023-11-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-023-06763-x |
| _version_ | 1797454061521338368 |
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| author | Tabitha N. Rosenbalm Nicole H. Levi Michael J. Morykwas William D. Wagner |
| author_facet | Tabitha N. Rosenbalm Nicole H. Levi Michael J. Morykwas William D. Wagner |
| author_sort | Tabitha N. Rosenbalm |
| collection | DOAJ |
| description | Abstract Improved materials for peripheral nerve repair are needed for the advancement of new surgical techniques in fields spanning from oncology to trauma. In this study, we developed bioresorbable materials capable of producing repeated electric field gradients spaced 600 μm apart to assess the impact on neuronal cell growth, and migration. Electrically conductive, biphasic composites comprised of poly (glycerol) sebacate acrylate (PGSA) alone, and doped with poly (pyrrole) (PPy), were prepared to create alternating segments with high and low electrically conductivity. Conductivity measurements demonstrated that 0.05% PPy added to PSA achieved an optimal value of 1.25 × 10−4 S/cm, for subsequent electrical stimulation. Tensile testing and degradation of PPy doped and undoped PGSA determined that 35–40% acrylation of PGSA matched nerve mechanical properties. Both fibroblast and neuronal cells thrived when cultured upon the composite. Biphasic PGSA/PPy sheets seeded with neuronal cells stimulated for with 3 V, 20 Hz demonstrated a 5x cell increase with 1 day of stimulation and up to a 10x cell increase with 3 days stimulation compared to non-stimulated composites. Tubular conduits composed of repeated high and low conductivity materials suitable for implantation in the rat sciatic nerve model for nerve repair were evaluated in vivo and were superior to silicone conduits. These results suggest that biphasic conducting conduits capable of maintaining mechanical properties without inducing compression injuries while generating repeated electric fields are a promising tool for acceleration of peripheral nerve repair to previously untreatable patients. Graphical Abstract |
| first_indexed | 2024-03-09T15:31:49Z |
| format | Article |
| id | doaj.art-15338620217e4e75b1273c0f283a873b |
| institution | Directory Open Access Journal |
| issn | 1573-4838 |
| language | English |
| last_indexed | 2024-03-09T15:31:49Z |
| publishDate | 2023-11-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj.art-15338620217e4e75b1273c0f283a873b2023-11-26T12:12:17ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382023-11-01341111810.1007/s10856-023-06763-xElectrical stimulation via repeated biphasic conducting materials for peripheral nerve regenerationTabitha N. Rosenbalm0Nicole H. Levi1Michael J. Morykwas2William D. Wagner3School of Biomedical Engineering and Sciences, Wake Forest University-Virginia Polytechnic Institute and State UniversitySchool of Biomedical Engineering and Sciences, Wake Forest University-Virginia Polytechnic Institute and State UniversitySchool of Biomedical Engineering and Sciences, Wake Forest University-Virginia Polytechnic Institute and State UniversitySchool of Biomedical Engineering and Sciences, Wake Forest University-Virginia Polytechnic Institute and State UniversityAbstract Improved materials for peripheral nerve repair are needed for the advancement of new surgical techniques in fields spanning from oncology to trauma. In this study, we developed bioresorbable materials capable of producing repeated electric field gradients spaced 600 μm apart to assess the impact on neuronal cell growth, and migration. Electrically conductive, biphasic composites comprised of poly (glycerol) sebacate acrylate (PGSA) alone, and doped with poly (pyrrole) (PPy), were prepared to create alternating segments with high and low electrically conductivity. Conductivity measurements demonstrated that 0.05% PPy added to PSA achieved an optimal value of 1.25 × 10−4 S/cm, for subsequent electrical stimulation. Tensile testing and degradation of PPy doped and undoped PGSA determined that 35–40% acrylation of PGSA matched nerve mechanical properties. Both fibroblast and neuronal cells thrived when cultured upon the composite. Biphasic PGSA/PPy sheets seeded with neuronal cells stimulated for with 3 V, 20 Hz demonstrated a 5x cell increase with 1 day of stimulation and up to a 10x cell increase with 3 days stimulation compared to non-stimulated composites. Tubular conduits composed of repeated high and low conductivity materials suitable for implantation in the rat sciatic nerve model for nerve repair were evaluated in vivo and were superior to silicone conduits. These results suggest that biphasic conducting conduits capable of maintaining mechanical properties without inducing compression injuries while generating repeated electric fields are a promising tool for acceleration of peripheral nerve repair to previously untreatable patients. Graphical Abstracthttps://doi.org/10.1007/s10856-023-06763-x |
| spellingShingle | Tabitha N. Rosenbalm Nicole H. Levi Michael J. Morykwas William D. Wagner Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration Journal of Materials Science: Materials in Medicine |
| title | Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
| title_full | Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
| title_fullStr | Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
| title_full_unstemmed | Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
| title_short | Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
| title_sort | electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration |
| url | https://doi.org/10.1007/s10856-023-06763-x |
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