A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts
<p>Abstract</p> <p>Background</p> <p>The p38α mitogen-activated protein kinase (MAPK) is a critical mediator of myoblast differentiation, and does so in part through the phosphorylation and regulation of several transcription factors and chromatin remodelling proteins....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-03-01
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| Series: | Skeletal Muscle |
| Subjects: | |
| Online Access: | http://www.skeletalmusclejournal.com/content/2/1/5 |
| _version_ | 1819025893077549056 |
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| author | Knight James DR Tian Ruijun Lee Robin EC Wang Fangjun Beauvais Ariane Zou Hanfa Megeney Lynn A Gingras Anne-Claude Pawson Tony Figeys Daniel Kothary Rashmi |
| author_facet | Knight James DR Tian Ruijun Lee Robin EC Wang Fangjun Beauvais Ariane Zou Hanfa Megeney Lynn A Gingras Anne-Claude Pawson Tony Figeys Daniel Kothary Rashmi |
| author_sort | Knight James DR |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The p38α mitogen-activated protein kinase (MAPK) is a critical mediator of myoblast differentiation, and does so in part through the phosphorylation and regulation of several transcription factors and chromatin remodelling proteins. However, whether p38α is involved in processes other than gene regulation during myogenesis is currently unknown, and why other p38 isoforms cannot compensate for its loss is unclear.</p> <p>Methods</p> <p>To further characterise the involvement of p38α during myoblast differentiation, we developed and applied a simple technique for identifying relevant <it>in vivo </it>kinase substrates and their phosphorylation sites. In addition to identifying substrates for one kinase, the technique can be used <it>in vitro </it>to compare multiple kinases in the same experiment, and we made use of this to study the substrate specificities of the p38α and β isoforms.</p> <p>Results</p> <p>Applying the technique to p38α resulted in the identification of seven <it>in vivo </it>phosphorylation sites on six proteins, four of which are cytoplasmic, in lysate derived from differentiating myoblasts. An <it>in vitro </it>comparison with p38β revealed that substrate specificity does not discriminate these two isoforms, but rather that their distinguishing characteristic appears to be cellular localisation.</p> <p>Conclusion</p> <p>Our results suggest p38α has a novel cytoplasmic role during myogenesis and that its unique cellular localisation may be why p38β and other isoforms cannot compensate for its absence. The substrate-finding approach presented here also provides a necessary tool for studying the hundreds of protein kinases that exist and for uncovering the deeper mechanisms of phosphorylation-dependent cell signalling.</p> |
| first_indexed | 2024-12-21T05:17:55Z |
| format | Article |
| id | doaj.art-15368bd4fa104ce7acf392a6d9f656c6 |
| institution | Directory Open Access Journal |
| issn | 2044-5040 |
| language | English |
| last_indexed | 2024-12-21T05:17:55Z |
| publishDate | 2012-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Skeletal Muscle |
| spelling | doaj.art-15368bd4fa104ce7acf392a6d9f656c62022-12-21T19:14:52ZengBMCSkeletal Muscle2044-50402012-03-0121510.1186/2044-5040-2-5A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblastsKnight James DRTian RuijunLee Robin ECWang FangjunBeauvais ArianeZou HanfaMegeney Lynn AGingras Anne-ClaudePawson TonyFigeys DanielKothary Rashmi<p>Abstract</p> <p>Background</p> <p>The p38α mitogen-activated protein kinase (MAPK) is a critical mediator of myoblast differentiation, and does so in part through the phosphorylation and regulation of several transcription factors and chromatin remodelling proteins. However, whether p38α is involved in processes other than gene regulation during myogenesis is currently unknown, and why other p38 isoforms cannot compensate for its loss is unclear.</p> <p>Methods</p> <p>To further characterise the involvement of p38α during myoblast differentiation, we developed and applied a simple technique for identifying relevant <it>in vivo </it>kinase substrates and their phosphorylation sites. In addition to identifying substrates for one kinase, the technique can be used <it>in vitro </it>to compare multiple kinases in the same experiment, and we made use of this to study the substrate specificities of the p38α and β isoforms.</p> <p>Results</p> <p>Applying the technique to p38α resulted in the identification of seven <it>in vivo </it>phosphorylation sites on six proteins, four of which are cytoplasmic, in lysate derived from differentiating myoblasts. An <it>in vitro </it>comparison with p38β revealed that substrate specificity does not discriminate these two isoforms, but rather that their distinguishing characteristic appears to be cellular localisation.</p> <p>Conclusion</p> <p>Our results suggest p38α has a novel cytoplasmic role during myogenesis and that its unique cellular localisation may be why p38β and other isoforms cannot compensate for its absence. The substrate-finding approach presented here also provides a necessary tool for studying the hundreds of protein kinases that exist and for uncovering the deeper mechanisms of phosphorylation-dependent cell signalling.</p>http://www.skeletalmusclejournal.com/content/2/1/5differentiationFSBAkinase assaymitogen-activated protein kinasemyoblastp38phosphorylationquantitative MS |
| spellingShingle | Knight James DR Tian Ruijun Lee Robin EC Wang Fangjun Beauvais Ariane Zou Hanfa Megeney Lynn A Gingras Anne-Claude Pawson Tony Figeys Daniel Kothary Rashmi A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts Skeletal Muscle differentiation FSBA kinase assay mitogen-activated protein kinase myoblast p38 phosphorylation quantitative MS |
| title | A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts |
| title_full | A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts |
| title_fullStr | A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts |
| title_full_unstemmed | A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts |
| title_short | A novel whole-cell lysate kinase assay identifies substrates of the p38 MAPK in differentiating myoblasts |
| title_sort | novel whole cell lysate kinase assay identifies substrates of the p38 mapk in differentiating myoblasts |
| topic | differentiation FSBA kinase assay mitogen-activated protein kinase myoblast p38 phosphorylation quantitative MS |
| url | http://www.skeletalmusclejournal.com/content/2/1/5 |
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