Effects on Fetal Metabolic Programming and Endocannabinoid System of a Normocaloric Diet during Pregnancy and Lactation of Female Mice with Pregestational Obesity
Fetal programming provides explanatory mechanisms for the currently high prevalence of gestational obesity. The endocannabinoid system (ECS) participates in the regulation of energy balance, and with a high-fat diet (HFD), it is overactivated. The aim of this study was to determine the effects of a...
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MDPI AG
2023-08-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/16/3531 |
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| author | Cynthia Barrera Valeska Castillo Rodrigo Valenzuela Carina A. Valenzuela Diego F. Garcia-Diaz Miguel Llanos |
| author_facet | Cynthia Barrera Valeska Castillo Rodrigo Valenzuela Carina A. Valenzuela Diego F. Garcia-Diaz Miguel Llanos |
| author_sort | Cynthia Barrera |
| collection | DOAJ |
| description | Fetal programming provides explanatory mechanisms for the currently high prevalence of gestational obesity. The endocannabinoid system (ECS) participates in the regulation of energy balance, and with a high-fat diet (HFD), it is overactivated. The aim of this study was to determine the effects of a nutritional intervention during pregnancy and lactation on obese female progenitors, on metabolic alterations of the offspring and on the involvement of ECS. Female mice (C57/BL/6-F0), 45 days old, and their offspring (males) were separated according to type of diet before and during gestation and lactation: CON-F1: control diet; HFD-F1 group: HFD (fat: 60% Kcal); INT-F1 group: HFD until mating and control diet (fat: 10% Kcal) afterward. Glucose tolerance and insulin sensitivity (IS) were tested at 2 and 4 months. At 120 days, mice were sacrificed, plasma was extracted for the determination of hormones, and livers for gene expression and the protein level determination of ECS components. INT-F1 group presented a lower IS compared to CON-F1, and normal levels of adiponectin and corticosterone in relation to the HFD-F1 group. The intervention increased hepatic gene expression for fatty-acid amide hydrolase and monoacylglycerol lipase enzymes; however, these differences were not observed at the protein expression level. Our results suggest that this intervention model normalized some hormonal parameters and hepatic mRNA levels of ECS components that were altered in the offspring of progenitors with pre-pregnancy obesity. |
| first_indexed | 2024-03-10T23:40:15Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T23:40:15Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
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| series | Nutrients |
| spelling | doaj.art-15381aa9728a4df9a3a55a8bf2d766a92023-11-19T02:29:14ZengMDPI AGNutrients2072-66432023-08-011516353110.3390/nu15163531Effects on Fetal Metabolic Programming and Endocannabinoid System of a Normocaloric Diet during Pregnancy and Lactation of Female Mice with Pregestational ObesityCynthia Barrera0Valeska Castillo1Rodrigo Valenzuela2Carina A. Valenzuela3Diego F. Garcia-Diaz4Miguel Llanos5Department of Nutrition, Faculty of Medicine, University of Chile, Santiago 8380000, ChileLaboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago 8380453, ChileDepartment of Nutrition, Faculty of Medicine, University of Chile, Santiago 8380000, ChileEating Behavior Research Center, School of Nutrition and Dietetics, Faculty of Pharmacy, Universidad de Valparaíso Playa Ancha, Valparaíso 2360102, ChileDepartment of Nutrition, Faculty of Medicine, University of Chile, Santiago 8380000, ChileLaboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago 8380453, ChileFetal programming provides explanatory mechanisms for the currently high prevalence of gestational obesity. The endocannabinoid system (ECS) participates in the regulation of energy balance, and with a high-fat diet (HFD), it is overactivated. The aim of this study was to determine the effects of a nutritional intervention during pregnancy and lactation on obese female progenitors, on metabolic alterations of the offspring and on the involvement of ECS. Female mice (C57/BL/6-F0), 45 days old, and their offspring (males) were separated according to type of diet before and during gestation and lactation: CON-F1: control diet; HFD-F1 group: HFD (fat: 60% Kcal); INT-F1 group: HFD until mating and control diet (fat: 10% Kcal) afterward. Glucose tolerance and insulin sensitivity (IS) were tested at 2 and 4 months. At 120 days, mice were sacrificed, plasma was extracted for the determination of hormones, and livers for gene expression and the protein level determination of ECS components. INT-F1 group presented a lower IS compared to CON-F1, and normal levels of adiponectin and corticosterone in relation to the HFD-F1 group. The intervention increased hepatic gene expression for fatty-acid amide hydrolase and monoacylglycerol lipase enzymes; however, these differences were not observed at the protein expression level. Our results suggest that this intervention model normalized some hormonal parameters and hepatic mRNA levels of ECS components that were altered in the offspring of progenitors with pre-pregnancy obesity.https://www.mdpi.com/2072-6643/15/16/3531obesitypregnancylactationfetal programmingnutritional interventionendocannabinoid system |
| spellingShingle | Cynthia Barrera Valeska Castillo Rodrigo Valenzuela Carina A. Valenzuela Diego F. Garcia-Diaz Miguel Llanos Effects on Fetal Metabolic Programming and Endocannabinoid System of a Normocaloric Diet during Pregnancy and Lactation of Female Mice with Pregestational Obesity Nutrients obesity pregnancy lactation fetal programming nutritional intervention endocannabinoid system |
| title | Effects on Fetal Metabolic Programming and Endocannabinoid System of a Normocaloric Diet during Pregnancy and Lactation of Female Mice with Pregestational Obesity |
| title_full | Effects on Fetal Metabolic Programming and Endocannabinoid System of a Normocaloric Diet during Pregnancy and Lactation of Female Mice with Pregestational Obesity |
| title_fullStr | Effects on Fetal Metabolic Programming and Endocannabinoid System of a Normocaloric Diet during Pregnancy and Lactation of Female Mice with Pregestational Obesity |
| title_full_unstemmed | Effects on Fetal Metabolic Programming and Endocannabinoid System of a Normocaloric Diet during Pregnancy and Lactation of Female Mice with Pregestational Obesity |
| title_short | Effects on Fetal Metabolic Programming and Endocannabinoid System of a Normocaloric Diet during Pregnancy and Lactation of Female Mice with Pregestational Obesity |
| title_sort | effects on fetal metabolic programming and endocannabinoid system of a normocaloric diet during pregnancy and lactation of female mice with pregestational obesity |
| topic | obesity pregnancy lactation fetal programming nutritional intervention endocannabinoid system |
| url | https://www.mdpi.com/2072-6643/15/16/3531 |
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