Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)
Abstract Serological cross-reactivity among flaviviruses makes determining the prior arbovirus exposure of animals challenging in areas where multiple flavivirus strains are circulating. We hypothesized that prior infection with ZIKV could be confirmed through the presence of subgenomic flavivirus R...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-87816-5 |
| _version_ | 1818431523321282560 |
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| author | Anna C. Fagre Juliette Lewis Megan R. Miller Eric C. Mossel Julius J. Lutwama Luke Nyakarahuka Teddy Nakayiki Robert Kityo Betty Nalikka Jonathan S. Towner Brian R. Amman Tara K. Sealy Brian Foy Tony Schountz John Anderson Rebekah C. Kading |
| author_facet | Anna C. Fagre Juliette Lewis Megan R. Miller Eric C. Mossel Julius J. Lutwama Luke Nyakarahuka Teddy Nakayiki Robert Kityo Betty Nalikka Jonathan S. Towner Brian R. Amman Tara K. Sealy Brian Foy Tony Schountz John Anderson Rebekah C. Kading |
| author_sort | Anna C. Fagre |
| collection | DOAJ |
| description | Abstract Serological cross-reactivity among flaviviruses makes determining the prior arbovirus exposure of animals challenging in areas where multiple flavivirus strains are circulating. We hypothesized that prior infection with ZIKV could be confirmed through the presence of subgenomic flavivirus RNA (sfRNA) of the 3′ untranslated region (UTR), which persists in tissues due to XRN-1 stalling during RNA decay. We amplified ZIKV sfRNA but not NS5 from three experimentally-infected Jamaican fruit bats, supporting the hypothesis of sfRNA tissue persistence. Applying this approach to 198 field samples from Uganda, we confirmed presence of ZIKV sfRNA, but not NS5, in four bats representing three species: Eidolon helvum (n = 2), Epomophorus labiatus (n = 1), and Rousettus aegyptiacus (n = 1). Amplified sequence was most closely related to Asian lineage ZIKV. Our results support the use of sfRNA as a means of identifying previous flavivirus infection and describe the first detection of ZIKV RNA in East African bats. |
| first_indexed | 2024-12-14T15:50:39Z |
| format | Article |
| id | doaj.art-153a44505c124faebb4f55fc624224a0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T15:50:39Z |
| publishDate | 2021-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-153a44505c124faebb4f55fc624224a02022-12-21T22:55:23ZengNature PortfolioScientific Reports2045-23222021-04-011111810.1038/s41598-021-87816-5Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae)Anna C. Fagre0Juliette Lewis1Megan R. Miller2Eric C. Mossel3Julius J. Lutwama4Luke Nyakarahuka5Teddy Nakayiki6Robert Kityo7Betty Nalikka8Jonathan S. Towner9Brian R. Amman10Tara K. Sealy11Brian Foy12Tony Schountz13John Anderson14Rebekah C. Kading15Colorado State UniversityColorado State UniversityColorado State UniversityCenters for Disease Control and PreventionUganda Virus Research InstituteUganda Virus Research InstituteUganda Virus Research InstituteMakerere UniversityMakerere UniversityCenters for Disease Control and PreventionCenters for Disease Control and PreventionCenters for Disease Control and PreventionColorado State UniversityColorado State UniversityColorado State UniversityColorado State UniversityAbstract Serological cross-reactivity among flaviviruses makes determining the prior arbovirus exposure of animals challenging in areas where multiple flavivirus strains are circulating. We hypothesized that prior infection with ZIKV could be confirmed through the presence of subgenomic flavivirus RNA (sfRNA) of the 3′ untranslated region (UTR), which persists in tissues due to XRN-1 stalling during RNA decay. We amplified ZIKV sfRNA but not NS5 from three experimentally-infected Jamaican fruit bats, supporting the hypothesis of sfRNA tissue persistence. Applying this approach to 198 field samples from Uganda, we confirmed presence of ZIKV sfRNA, but not NS5, in four bats representing three species: Eidolon helvum (n = 2), Epomophorus labiatus (n = 1), and Rousettus aegyptiacus (n = 1). Amplified sequence was most closely related to Asian lineage ZIKV. Our results support the use of sfRNA as a means of identifying previous flavivirus infection and describe the first detection of ZIKV RNA in East African bats.https://doi.org/10.1038/s41598-021-87816-5 |
| spellingShingle | Anna C. Fagre Juliette Lewis Megan R. Miller Eric C. Mossel Julius J. Lutwama Luke Nyakarahuka Teddy Nakayiki Robert Kityo Betty Nalikka Jonathan S. Towner Brian R. Amman Tara K. Sealy Brian Foy Tony Schountz John Anderson Rebekah C. Kading Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae) Scientific Reports |
| title | Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae) |
| title_full | Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae) |
| title_fullStr | Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae) |
| title_full_unstemmed | Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae) |
| title_short | Subgenomic flavivirus RNA (sfRNA) associated with Asian lineage Zika virus identified in three species of Ugandan bats (family Pteropodidae) |
| title_sort | subgenomic flavivirus rna sfrna associated with asian lineage zika virus identified in three species of ugandan bats family pteropodidae |
| url | https://doi.org/10.1038/s41598-021-87816-5 |
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