Identification of human NK17/NK1 cells.
BACKGROUND: Natural killer (NK) cells have both cytolytic and immunoregulatory functions. We recently described that these cells release the inflammatory cytokines IL-17 and IFN-γ. However, the precise identity of the NK cell subset(s) that secrete these cytokines is not known. METHODOLOGY/PRINCIPAL...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3198820?pdf=render |
| _version_ | 1818610754566225920 |
|---|---|
| author | Abhilash D Pandya Zaidoon Al-Jaderi Rune A Høglund Trygve Holmøy Hanne F Harbo Johannes Norgauer Azzam A Maghazachi |
| author_facet | Abhilash D Pandya Zaidoon Al-Jaderi Rune A Høglund Trygve Holmøy Hanne F Harbo Johannes Norgauer Azzam A Maghazachi |
| author_sort | Abhilash D Pandya |
| collection | DOAJ |
| description | BACKGROUND: Natural killer (NK) cells have both cytolytic and immunoregulatory functions. We recently described that these cells release the inflammatory cytokines IL-17 and IFN-γ. However, the precise identity of the NK cell subset(s) that secrete these cytokines is not known. METHODOLOGY/PRINCIPAL FINDINGS: To isolate the cells secreting IL-17 and IFN-γ, we took advantage of the findings that Th17/Th1 cells express chemokine receptors. Therefore, CD56(+)NK cells were stained with antibodies against various chemokine receptors and intracellularly with antibodies toward IL-17 and IFN-γ. Consequently, we identified previously unrecognized subset of NK cells generated from normal human peripheral blood after activation with IL-2 but not PMA plus ionomycin. The cells are characterized by the expression of CD56(+) and CCR4(+), produce IL-17 and IFN-γ and are consequently named NK17/NK1 cells. They also express CD161, NKp30, NKp44, NKp46, NKG2D, CD158, CCL22, IL-2Rβ and the common γ chain but not CD127 or IL-23R. Further, they possess T-bet and RORγt transcription factors. Antibodies to IL-1β, IL-6, IL-21, or TGF-β1 do not inhibit IL-2-induced generation of NK17/NK1 cells, suggesting that IL-2 has the capacity to polarize these cells. Notably, NK17/NK1 cells are abundant in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) without activation, and are generated from the peripheral blood of these patients after activation with IL-2. CONCLUSIONS/SIGNIFICANCE: NK17/NK1 cells identified here have not been previously described in healthy or MS patients. |
| first_indexed | 2024-12-16T15:19:28Z |
| format | Article |
| id | doaj.art-153c7a7a2c914069b79ec57e9abff285 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T15:19:28Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-153c7a7a2c914069b79ec57e9abff2852022-12-21T22:26:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2678010.1371/journal.pone.0026780Identification of human NK17/NK1 cells.Abhilash D PandyaZaidoon Al-JaderiRune A HøglundTrygve HolmøyHanne F HarboJohannes NorgauerAzzam A MaghazachiBACKGROUND: Natural killer (NK) cells have both cytolytic and immunoregulatory functions. We recently described that these cells release the inflammatory cytokines IL-17 and IFN-γ. However, the precise identity of the NK cell subset(s) that secrete these cytokines is not known. METHODOLOGY/PRINCIPAL FINDINGS: To isolate the cells secreting IL-17 and IFN-γ, we took advantage of the findings that Th17/Th1 cells express chemokine receptors. Therefore, CD56(+)NK cells were stained with antibodies against various chemokine receptors and intracellularly with antibodies toward IL-17 and IFN-γ. Consequently, we identified previously unrecognized subset of NK cells generated from normal human peripheral blood after activation with IL-2 but not PMA plus ionomycin. The cells are characterized by the expression of CD56(+) and CCR4(+), produce IL-17 and IFN-γ and are consequently named NK17/NK1 cells. They also express CD161, NKp30, NKp44, NKp46, NKG2D, CD158, CCL22, IL-2Rβ and the common γ chain but not CD127 or IL-23R. Further, they possess T-bet and RORγt transcription factors. Antibodies to IL-1β, IL-6, IL-21, or TGF-β1 do not inhibit IL-2-induced generation of NK17/NK1 cells, suggesting that IL-2 has the capacity to polarize these cells. Notably, NK17/NK1 cells are abundant in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) without activation, and are generated from the peripheral blood of these patients after activation with IL-2. CONCLUSIONS/SIGNIFICANCE: NK17/NK1 cells identified here have not been previously described in healthy or MS patients.http://europepmc.org/articles/PMC3198820?pdf=render |
| spellingShingle | Abhilash D Pandya Zaidoon Al-Jaderi Rune A Høglund Trygve Holmøy Hanne F Harbo Johannes Norgauer Azzam A Maghazachi Identification of human NK17/NK1 cells. PLoS ONE |
| title | Identification of human NK17/NK1 cells. |
| title_full | Identification of human NK17/NK1 cells. |
| title_fullStr | Identification of human NK17/NK1 cells. |
| title_full_unstemmed | Identification of human NK17/NK1 cells. |
| title_short | Identification of human NK17/NK1 cells. |
| title_sort | identification of human nk17 nk1 cells |
| url | http://europepmc.org/articles/PMC3198820?pdf=render |
| work_keys_str_mv | AT abhilashdpandya identificationofhumannk17nk1cells AT zaidoonaljaderi identificationofhumannk17nk1cells AT runeahøglund identificationofhumannk17nk1cells AT trygveholmøy identificationofhumannk17nk1cells AT hannefharbo identificationofhumannk17nk1cells AT johannesnorgauer identificationofhumannk17nk1cells AT azzamamaghazachi identificationofhumannk17nk1cells |