RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean
The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to t...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2002-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300012 |
| _version_ | 1831741552211263488 |
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| author | Sebastião Martins Filho Carlos Sigueyuki Sediyama Maurilio Alves Moreira Everaldo Gonçalves de Barros |
| author_facet | Sebastião Martins Filho Carlos Sigueyuki Sediyama Maurilio Alves Moreira Everaldo Gonçalves de Barros |
| author_sort | Sebastião Martins Filho |
| collection | DOAJ |
| description | The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross 'Parana' x 'Bossier', 2 RAPD markers were identified, CSOPA1(800C) and CSOPA2(1,250C), located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the resistance locus. DNA fragments of similar molecular weight were observed in the population derived from the cross 'Cristalina' x 'Bossier' at 2.3 ± 1.2 and 4.7 ± 1.5 cM from the resistance locus, respectively. In the offspring of the cross 'Uberaba' x 'Bossier', a DNA fragment corresponding to marker CSOPA1(800C) was detected at 5.6 ± 2.1 cM from the resistance locus. Although marker CSOPA2(1,250C) was not observed in this population, an additional marker was detected (CSOUB1(1,100C)) at 6.7 ± 2.2 cM from the resistance locus. The 1,250 bp fragment of CSOPA2(1,250C) was cloned and converted into a SCAR marker, which amplified a single fragment whose size corresponded to the cloned segment of the crosses involving cultivars Cristalina and Parana. Markers CSOPA1(800C), CSOPA2(1,250C) and CSOUB1(1,100C) were mapped to soybean linkage group J with the aid of known SSRs linked to the Rcs3 locus, indicating that the RAPD and SCAR markers identified in our research also tag this resistance gene. |
| first_indexed | 2024-12-21T14:17:37Z |
| format | Article |
| id | doaj.art-153e0eb0c80442d29146cc06f748ec61 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-21T14:17:37Z |
| publishDate | 2002-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-153e0eb0c80442d29146cc06f748ec612022-12-21T19:00:51ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852002-01-0125331732110.1590/S1415-47572002000300012RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybeanSebastião Martins FilhoCarlos Sigueyuki SediyamaMaurilio Alves MoreiraEveraldo Gonçalves de BarrosThe soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross 'Parana' x 'Bossier', 2 RAPD markers were identified, CSOPA1(800C) and CSOPA2(1,250C), located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the resistance locus. DNA fragments of similar molecular weight were observed in the population derived from the cross 'Cristalina' x 'Bossier' at 2.3 ± 1.2 and 4.7 ± 1.5 cM from the resistance locus, respectively. In the offspring of the cross 'Uberaba' x 'Bossier', a DNA fragment corresponding to marker CSOPA1(800C) was detected at 5.6 ± 2.1 cM from the resistance locus. Although marker CSOPA2(1,250C) was not observed in this population, an additional marker was detected (CSOUB1(1,100C)) at 6.7 ± 2.2 cM from the resistance locus. The 1,250 bp fragment of CSOPA2(1,250C) was cloned and converted into a SCAR marker, which amplified a single fragment whose size corresponded to the cloned segment of the crosses involving cultivars Cristalina and Parana. Markers CSOPA1(800C), CSOPA2(1,250C) and CSOUB1(1,100C) were mapped to soybean linkage group J with the aid of known SSRs linked to the Rcs3 locus, indicating that the RAPD and SCAR markers identified in our research also tag this resistance gene.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300012molecular markersRAPDSCARsoybeanCercospora sojinafrogeye leaf spot |
| spellingShingle | Sebastião Martins Filho Carlos Sigueyuki Sediyama Maurilio Alves Moreira Everaldo Gonçalves de Barros RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean Genetics and Molecular Biology molecular markers RAPD SCAR soybean Cercospora sojina frogeye leaf spot |
| title | RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean |
| title_full | RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean |
| title_fullStr | RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean |
| title_full_unstemmed | RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean |
| title_short | RAPD and SCAR markers linked to resistance to frogeye leaf spot in soybean |
| title_sort | rapd and scar markers linked to resistance to frogeye leaf spot in soybean |
| topic | molecular markers RAPD SCAR soybean Cercospora sojina frogeye leaf spot |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300012 |
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