24-Epibrassinolide promotes NO3 − and NH4 + ion flux rate and NRT1 gene expression in cucumber under suboptimal root zone temperature
Abstract Background Suboptimal root zone temperature (RZT) causes a remarkable reduction in growth of horticultural crops during winter cultivation under greenhouse production. However, limited information is available on the effects of suboptimal RZT on nitrogen (N) metabolism in cucumber seedlings...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1838-3 |
| _version_ | 1818201826461220864 |
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| author | Ali Anwar Yansu Li Chaoxing He Xianchang Yu |
| author_facet | Ali Anwar Yansu Li Chaoxing He Xianchang Yu |
| author_sort | Ali Anwar |
| collection | DOAJ |
| description | Abstract Background Suboptimal root zone temperature (RZT) causes a remarkable reduction in growth of horticultural crops during winter cultivation under greenhouse production. However, limited information is available on the effects of suboptimal RZT on nitrogen (N) metabolism in cucumber seedlings. The aim of this study is to investigate the effects of 24-Epibrassinolide (EBR) on nitrate and ammonium flux rate, N metabolism, and transcript levels of NRT1 family genes under suboptimal RZT in cucumber seedlings. Results Suboptimal RZT (LT) negatively affected on cucumber growth and proportionately decreased EBR contents, bleeding rate, root activity, enzyme activities of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), and glutamate synthase (GOGAT), nitrate (NO3 −) influx rate, ammonium (NH4 +) efflux rate, and transcript levels of nitrate transporter (NRT1) encoding genes. However, exogenous EBR reduced the harmful effects of suboptimal RZT and increased endogenous EBR contents, bleeding rate, root activity, enzyme activities of NR, NiR, GS, and GOGAT, NH4 + and NO3 − flux rates and contents, and N accumulation. EBR-treated seedlings also upregulated the transcript levels of nitrate transporters CsNRT1.1, CsNRT1.2A, CsNRT1.2B, CsNRT1.2C, CsNRT1.3, CsNRT1.4A, CsNRT1.5B, CsNRT1.5C, CsNRT1.9, and CsNRT1.10, and downregulated CsNRT1.5A and CsNRT1.8. LT treatment upregulated the expression level of CsNRT1.5A, while exogenous BZR application downregulated the expression level of NRT1 genes. Conclusion These results indicate that exogenous application of EBR alleviated the harmful effects of suboptimal RZT through changes in N metabolism, NH4 + and NO3 − flux rates, and NRT1 gene expression, leading to improved cucumber seedlings growth. Our study provides the first evidence of the role of EBR in the response to suboptimal RZT in cucumber, and can be used to improve vegetable production. |
| first_indexed | 2024-12-12T02:59:43Z |
| format | Article |
| id | doaj.art-153f0d60884142daa5ea66ea5ce05a7b |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-12T02:59:43Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-153f0d60884142daa5ea66ea5ce05a7b2022-12-22T00:40:39ZengBMCBMC Plant Biology1471-22292019-05-0119111510.1186/s12870-019-1838-324-Epibrassinolide promotes NO3 − and NH4 + ion flux rate and NRT1 gene expression in cucumber under suboptimal root zone temperatureAli Anwar0Yansu Li1Chaoxing He2Xianchang Yu3The Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesAbstract Background Suboptimal root zone temperature (RZT) causes a remarkable reduction in growth of horticultural crops during winter cultivation under greenhouse production. However, limited information is available on the effects of suboptimal RZT on nitrogen (N) metabolism in cucumber seedlings. The aim of this study is to investigate the effects of 24-Epibrassinolide (EBR) on nitrate and ammonium flux rate, N metabolism, and transcript levels of NRT1 family genes under suboptimal RZT in cucumber seedlings. Results Suboptimal RZT (LT) negatively affected on cucumber growth and proportionately decreased EBR contents, bleeding rate, root activity, enzyme activities of nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), and glutamate synthase (GOGAT), nitrate (NO3 −) influx rate, ammonium (NH4 +) efflux rate, and transcript levels of nitrate transporter (NRT1) encoding genes. However, exogenous EBR reduced the harmful effects of suboptimal RZT and increased endogenous EBR contents, bleeding rate, root activity, enzyme activities of NR, NiR, GS, and GOGAT, NH4 + and NO3 − flux rates and contents, and N accumulation. EBR-treated seedlings also upregulated the transcript levels of nitrate transporters CsNRT1.1, CsNRT1.2A, CsNRT1.2B, CsNRT1.2C, CsNRT1.3, CsNRT1.4A, CsNRT1.5B, CsNRT1.5C, CsNRT1.9, and CsNRT1.10, and downregulated CsNRT1.5A and CsNRT1.8. LT treatment upregulated the expression level of CsNRT1.5A, while exogenous BZR application downregulated the expression level of NRT1 genes. Conclusion These results indicate that exogenous application of EBR alleviated the harmful effects of suboptimal RZT through changes in N metabolism, NH4 + and NO3 − flux rates, and NRT1 gene expression, leading to improved cucumber seedlings growth. Our study provides the first evidence of the role of EBR in the response to suboptimal RZT in cucumber, and can be used to improve vegetable production.http://link.springer.com/article/10.1186/s12870-019-1838-324-EpibrassinolideRoot zone temperatureNO3 − and NH4 + flux ratesNRT1 genes |
| spellingShingle | Ali Anwar Yansu Li Chaoxing He Xianchang Yu 24-Epibrassinolide promotes NO3 − and NH4 + ion flux rate and NRT1 gene expression in cucumber under suboptimal root zone temperature BMC Plant Biology 24-Epibrassinolide Root zone temperature NO3 − and NH4 + flux rates NRT1 genes |
| title | 24-Epibrassinolide promotes NO3 − and NH4 + ion flux rate and NRT1 gene expression in cucumber under suboptimal root zone temperature |
| title_full | 24-Epibrassinolide promotes NO3 − and NH4 + ion flux rate and NRT1 gene expression in cucumber under suboptimal root zone temperature |
| title_fullStr | 24-Epibrassinolide promotes NO3 − and NH4 + ion flux rate and NRT1 gene expression in cucumber under suboptimal root zone temperature |
| title_full_unstemmed | 24-Epibrassinolide promotes NO3 − and NH4 + ion flux rate and NRT1 gene expression in cucumber under suboptimal root zone temperature |
| title_short | 24-Epibrassinolide promotes NO3 − and NH4 + ion flux rate and NRT1 gene expression in cucumber under suboptimal root zone temperature |
| title_sort | 24 epibrassinolide promotes no3 and nh4 ion flux rate and nrt1 gene expression in cucumber under suboptimal root zone temperature |
| topic | 24-Epibrassinolide Root zone temperature NO3 − and NH4 + flux rates NRT1 genes |
| url | http://link.springer.com/article/10.1186/s12870-019-1838-3 |
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