Supplementation of Alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa
Abstract This research was designed to explore the protective effect of alpha-lipoic acid–loaded nanoliposomes (ALAN) during cryopreservation of buffalo sperm. Buffalo semen was cryopreserved in a tris-citrate egg yolk extender without any supplement (ALAN0, control group) or with ALAN at levels of...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-26960-y |
| _version_ | 1797973614179385344 |
|---|---|
| author | Mahmoud A. E. Hassan Wael A. Khalil Sameh A. Abdelnour Reham Mokhtar Aman |
| author_facet | Mahmoud A. E. Hassan Wael A. Khalil Sameh A. Abdelnour Reham Mokhtar Aman |
| author_sort | Mahmoud A. E. Hassan |
| collection | DOAJ |
| description | Abstract This research was designed to explore the protective effect of alpha-lipoic acid–loaded nanoliposomes (ALAN) during cryopreservation of buffalo sperm. Buffalo semen was cryopreserved in a tris-citrate egg yolk extender without any supplement (ALAN0, control group) or with ALAN at levels of 25, 50, 75 or 150 µg (ALAN25, ALAN50, ALAN75 and ALAN150, respectively). The ALAN had a size of 171.80 nm and a negative zeta potential (− 43.40 mV). The progressive motility, vitality and membrane integrity significantly improved in all ALAN groups (except ALAN25 for membrane integrity). ALAN150 group exhibited the best values of progressive sperm motility, vitality and membrane integrity after thawing at 37 °C for 30 s or incubated for 2 h at 37 °C and 5% CO2 compared with those in other groups. Both ALAN75 and ALAN150 groups significantly improved the TAC, GR and catalase, while lipid peroxidation and early apoptotic spermatozoa significantly decreased in ALAN150 group followed by ALAN75 group. Collectively, the adding ALAN to buffalo semen freezing extender plays a substantial shielding function against cryodamage by preserving the sperm functional parameters. |
| first_indexed | 2024-04-11T04:05:56Z |
| format | Article |
| id | doaj.art-153fdaee0e9c4a208cb561a26bbe193b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T04:05:56Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-153fdaee0e9c4a208cb561a26bbe193b2023-01-01T12:20:18ZengNature PortfolioScientific Reports2045-23222022-12-0112111510.1038/s41598-022-26960-ySupplementation of Alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoaMahmoud A. E. Hassan0Wael A. Khalil1Sameh A. Abdelnour2Reham Mokhtar Aman3Animal Production Research Institute, Agriculture Research Centre, Ministry of AgricultureDepartment of Animal Production, Faculty of Agriculture, Mansoura UniversityDepartment of Animal Production, Faculty of Agriculture, Zagazig UniversityDepartment of Pharmaceutics, Faculty of Pharmacy, Mansoura UniversityAbstract This research was designed to explore the protective effect of alpha-lipoic acid–loaded nanoliposomes (ALAN) during cryopreservation of buffalo sperm. Buffalo semen was cryopreserved in a tris-citrate egg yolk extender without any supplement (ALAN0, control group) or with ALAN at levels of 25, 50, 75 or 150 µg (ALAN25, ALAN50, ALAN75 and ALAN150, respectively). The ALAN had a size of 171.80 nm and a negative zeta potential (− 43.40 mV). The progressive motility, vitality and membrane integrity significantly improved in all ALAN groups (except ALAN25 for membrane integrity). ALAN150 group exhibited the best values of progressive sperm motility, vitality and membrane integrity after thawing at 37 °C for 30 s or incubated for 2 h at 37 °C and 5% CO2 compared with those in other groups. Both ALAN75 and ALAN150 groups significantly improved the TAC, GR and catalase, while lipid peroxidation and early apoptotic spermatozoa significantly decreased in ALAN150 group followed by ALAN75 group. Collectively, the adding ALAN to buffalo semen freezing extender plays a substantial shielding function against cryodamage by preserving the sperm functional parameters.https://doi.org/10.1038/s41598-022-26960-y |
| spellingShingle | Mahmoud A. E. Hassan Wael A. Khalil Sameh A. Abdelnour Reham Mokhtar Aman Supplementation of Alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa Scientific Reports |
| title | Supplementation of Alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa |
| title_full | Supplementation of Alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa |
| title_fullStr | Supplementation of Alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa |
| title_full_unstemmed | Supplementation of Alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa |
| title_short | Supplementation of Alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa |
| title_sort | supplementation of alpha lipoic acid loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa |
| url | https://doi.org/10.1038/s41598-022-26960-y |
| work_keys_str_mv | AT mahmoudaehassan supplementationofalphalipoicacidloadednanoliposomesinsemenextenderimprovesfreezabilityofbuffalospermatozoa AT waelakhalil supplementationofalphalipoicacidloadednanoliposomesinsemenextenderimprovesfreezabilityofbuffalospermatozoa AT samehaabdelnour supplementationofalphalipoicacidloadednanoliposomesinsemenextenderimprovesfreezabilityofbuffalospermatozoa AT rehammokhtaraman supplementationofalphalipoicacidloadednanoliposomesinsemenextenderimprovesfreezabilityofbuffalospermatozoa |