Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations
In the present study we standardized an experimental model of parabiotic circulation of isolated pig heart. The isolated heart was perfused with arterial blood from a second animal as support and submitted to regional ischemia for 30 min, followed by total ischemia for 90 min and reperfusion for 90...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2003-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500013 |
| _version_ | 1828808474114392064 |
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| author | Petrucci Júnior O. Oliveira P.P.M. Carmo M.R. Vieira R.W. Braile D.M. |
| author_facet | Petrucci Júnior O. Oliveira P.P.M. Carmo M.R. Vieira R.W. Braile D.M. |
| author_sort | Petrucci Júnior O. |
| collection | DOAJ |
| description | In the present study we standardized an experimental model of parabiotic circulation of isolated pig heart. The isolated heart was perfused with arterial blood from a second animal as support and submitted to regional ischemia for 30 min, followed by total ischemia for 90 min and reperfusion for 90 min. Parameters for measurement of ventricular performance using different indices measured directly or indirectly from intraventricular pressure were defined as: maximum peak pressure, final diastolic pressure, pressure developed, first derivative of maximum pressure (dP/dt max), first derivative of minimum pressure (dP/dt min), systolic stress of the left ventricle (sigmas), and maximum elastance of the left ventricle. Isolated hearts subjected to regional and global ischemia presented significant worsening of all measured parameters. Less discriminative parameters were dP/dt max and dP/dt min. Elastance was the most sensitive parameter during the reperfusion period, demonstrating an early loss of ventricular function during reperfusion. The model proved to be stable and reproducible and permitted the study of several variables in the isolated heart, such as ischemia and reperfusion phenomena, the effects of different drugs, surgical interventions, etc. The model introduces an advantage over the classical models which use crystalloid solutions as perfusate, because parabiotic circulation mimics heart surgery with extracorporeal circulation. |
| first_indexed | 2024-12-12T08:41:16Z |
| format | Article |
| id | doaj.art-1541595405eb4e32b279dc483a419121 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 0034-7310 |
| language | English |
| last_indexed | 2024-12-12T08:41:16Z |
| publishDate | 2003-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-1541595405eb4e32b279dc483a4191212022-12-22T00:30:45ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73102003-01-01365649659Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerationsPetrucci Júnior O.Oliveira P.P.M.Carmo M.R.Vieira R.W.Braile D.M.In the present study we standardized an experimental model of parabiotic circulation of isolated pig heart. The isolated heart was perfused with arterial blood from a second animal as support and submitted to regional ischemia for 30 min, followed by total ischemia for 90 min and reperfusion for 90 min. Parameters for measurement of ventricular performance using different indices measured directly or indirectly from intraventricular pressure were defined as: maximum peak pressure, final diastolic pressure, pressure developed, first derivative of maximum pressure (dP/dt max), first derivative of minimum pressure (dP/dt min), systolic stress of the left ventricle (sigmas), and maximum elastance of the left ventricle. Isolated hearts subjected to regional and global ischemia presented significant worsening of all measured parameters. Less discriminative parameters were dP/dt max and dP/dt min. Elastance was the most sensitive parameter during the reperfusion period, demonstrating an early loss of ventricular function during reperfusion. The model proved to be stable and reproducible and permitted the study of several variables in the isolated heart, such as ischemia and reperfusion phenomena, the effects of different drugs, surgical interventions, etc. The model introduces an advantage over the classical models which use crystalloid solutions as perfusate, because parabiotic circulation mimics heart surgery with extracorporeal circulation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500013Isolated heartIschemiaParabiotic circulationReperfusionModel |
| spellingShingle | Petrucci Júnior O. Oliveira P.P.M. Carmo M.R. Vieira R.W. Braile D.M. Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations Brazilian Journal of Medical and Biological Research Isolated heart Ischemia Parabiotic circulation Reperfusion Model |
| title | Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations |
| title_full | Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations |
| title_fullStr | Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations |
| title_full_unstemmed | Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations |
| title_short | Standardization of an isolated pig heart preparation with parabiotic circulation: methodological considerations |
| title_sort | standardization of an isolated pig heart preparation with parabiotic circulation methodological considerations |
| topic | Isolated heart Ischemia Parabiotic circulation Reperfusion Model |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500013 |
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