Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.
<h4>Background</h4>Mycolactones are toxins secreted by M. ulcerans, the etiological agent of Buruli ulcer. These toxins, which are the main virulence factors of the bacilli, are responsible for skin lesions. Considering their specificity for M. ulcerans and their presence in skin lesions...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22514607/?tool=EBI |
| _version_ | 1829486510004502528 |
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| author | Estelle Marion Soizic Prado Camille Cano Jérémie Babonneau Sarah Ghamrawi Laurent Marsollier |
| author_facet | Estelle Marion Soizic Prado Camille Cano Jérémie Babonneau Sarah Ghamrawi Laurent Marsollier |
| author_sort | Estelle Marion |
| collection | DOAJ |
| description | <h4>Background</h4>Mycolactones are toxins secreted by M. ulcerans, the etiological agent of Buruli ulcer. These toxins, which are the main virulence factors of the bacilli, are responsible for skin lesions. Considering their specificity for M. ulcerans and their presence in skin lesions even at early stages, mycolactones are promising candidates for the development of a diagnostic tool for M. ulcerans infection. Stability of purified mycolactones towards light and heat has not yet been investigated, despite the importance of such parameters in the selection of strategies for a diagnosis tool development. In this context, the effects of UV, light and temperature on mycolactone stability and biological activity were studied.<h4>Methodology/principal findings</h4>To investigate the effect of these physical parameters, mycolactones were exposed to different wavelengths in several solvents and temperatures. Structural changes and biological activity were monitored. Whilst high temperature had no effect on mycolactones, UV irradiation (UV-A, UV-B and UV-C) and sunlight exposure caused a considerable degradation, as revealed by LC-MS and NMR analysis, correlated with a loss of biological activity. Moreover, effect of UVs on mycolactone caused a photodegradation rather than a phototransformation due to the identification of degradation product.<h4>Conclusion/significance</h4>This study demonstrates the high sensitivity of mycolactones to UVs as such it defines instructions for storage and handling. |
| first_indexed | 2024-12-14T23:08:44Z |
| format | Article |
| id | doaj.art-15464e47bb414a3ca069da780b8ddfc0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T23:08:44Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-15464e47bb414a3ca069da780b8ddfc02022-12-21T22:44:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3360010.1371/journal.pone.0033600Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage.Estelle MarionSoizic PradoCamille CanoJérémie BabonneauSarah GhamrawiLaurent Marsollier<h4>Background</h4>Mycolactones are toxins secreted by M. ulcerans, the etiological agent of Buruli ulcer. These toxins, which are the main virulence factors of the bacilli, are responsible for skin lesions. Considering their specificity for M. ulcerans and their presence in skin lesions even at early stages, mycolactones are promising candidates for the development of a diagnostic tool for M. ulcerans infection. Stability of purified mycolactones towards light and heat has not yet been investigated, despite the importance of such parameters in the selection of strategies for a diagnosis tool development. In this context, the effects of UV, light and temperature on mycolactone stability and biological activity were studied.<h4>Methodology/principal findings</h4>To investigate the effect of these physical parameters, mycolactones were exposed to different wavelengths in several solvents and temperatures. Structural changes and biological activity were monitored. Whilst high temperature had no effect on mycolactones, UV irradiation (UV-A, UV-B and UV-C) and sunlight exposure caused a considerable degradation, as revealed by LC-MS and NMR analysis, correlated with a loss of biological activity. Moreover, effect of UVs on mycolactone caused a photodegradation rather than a phototransformation due to the identification of degradation product.<h4>Conclusion/significance</h4>This study demonstrates the high sensitivity of mycolactones to UVs as such it defines instructions for storage and handling.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22514607/?tool=EBI |
| spellingShingle | Estelle Marion Soizic Prado Camille Cano Jérémie Babonneau Sarah Ghamrawi Laurent Marsollier Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage. PLoS ONE |
| title | Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage. |
| title_full | Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage. |
| title_fullStr | Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage. |
| title_full_unstemmed | Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage. |
| title_short | Photodegradation of the Mycobacterium ulcerans toxin, mycolactones: considerations for handling and storage. |
| title_sort | photodegradation of the mycobacterium ulcerans toxin mycolactones considerations for handling and storage |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22514607/?tool=EBI |
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