A DNA barcode reference library for endemic Ponto-Caspian amphipods
Abstract The Ponto-Caspian region is an endemicity hotspot that harbours several crustacean radiations, among which amphipods are the most diverse. These poorly known species are severely threatened in their native range, while at the same time they are invading European inland waters with significa...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-15442-w |
| _version_ | 1818519316151140352 |
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| author | Denis Copilaş-Ciocianu Tomasz Rewicz Arthur F. Sands Dmitry Palatov Ivan Marin Kęstutis Arbačiauskas Paul D. N. Hebert Michal Grabowski Asta Audzijonyte |
| author_facet | Denis Copilaş-Ciocianu Tomasz Rewicz Arthur F. Sands Dmitry Palatov Ivan Marin Kęstutis Arbačiauskas Paul D. N. Hebert Michal Grabowski Asta Audzijonyte |
| author_sort | Denis Copilaş-Ciocianu |
| collection | DOAJ |
| description | Abstract The Ponto-Caspian region is an endemicity hotspot that harbours several crustacean radiations, among which amphipods are the most diverse. These poorly known species are severely threatened in their native range, while at the same time they are invading European inland waters with significant ecological consequences. A proper taxonomic knowledge of this fauna is paramount for its conservation within the native region and monitoring outside of it. Here, we assemble a DNA barcode reference library for nearly 60% of all known Ponto-Caspian amphipod species. We use several methods to define molecular operational taxonomic units (MOTUs), based on two mitochondrial markers (COI and 16S), and assess their congruence with current species-level taxonomy based on morphology. Depending on the method, we find that 54–69% of species had congruent morpho-molecular boundaries. The cases of incongruence resulted from lumping distinct morphospecies into a single MOTU (7–27%), splitting a morphospecies into several MOTUs (4–28%), or both (4–11%). MOTUs defined by distance-based methods without a priori divergence thresholds showed the highest congruence with morphological taxonomy. These results indicate that DNA barcoding is valuable for clarifying the diversity of Ponto-Caspian amphipods, but reveals that extensive work is needed to resolve taxonomic uncertainties. Our study advances the DNA barcode reference library for the European aquatic biota, paving the way towards improved taxonomic knowledge needed to enhance monitoring and conservation efforts. |
| first_indexed | 2024-12-11T01:22:31Z |
| format | Article |
| id | doaj.art-1546773856f246e59ce77c241dd2eaec |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-11T01:22:31Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1546773856f246e59ce77c241dd2eaec2022-12-22T01:25:40ZengNature PortfolioScientific Reports2045-23222022-07-0112111410.1038/s41598-022-15442-wA DNA barcode reference library for endemic Ponto-Caspian amphipodsDenis Copilaş-Ciocianu0Tomasz Rewicz1Arthur F. Sands2Dmitry Palatov3Ivan Marin4Kęstutis Arbačiauskas5Paul D. N. Hebert6Michal Grabowski7Asta Audzijonyte8Laboratory of Evolutionary Ecology of Hydrobionts, Nature Research CentreDepartment of Invertebrate Zoology and Hydrobiology, Faculty of Biology and Environmental Protection, University of LodzArea of Ecology and Biodiversity, School of Biological Sciences, University of Hong KongA.N. Severtsov Institute of Ecology and Evolution of RASA.N. Severtsov Institute of Ecology and Evolution of RASLaboratory of Evolutionary Ecology of Hydrobionts, Nature Research CentreCentre for Biodiversity Genomics, University of GuelphDepartment of Invertebrate Zoology and Hydrobiology, Faculty of Biology and Environmental Protection, University of LodzLaboratory of Evolutionary Ecology of Hydrobionts, Nature Research CentreAbstract The Ponto-Caspian region is an endemicity hotspot that harbours several crustacean radiations, among which amphipods are the most diverse. These poorly known species are severely threatened in their native range, while at the same time they are invading European inland waters with significant ecological consequences. A proper taxonomic knowledge of this fauna is paramount for its conservation within the native region and monitoring outside of it. Here, we assemble a DNA barcode reference library for nearly 60% of all known Ponto-Caspian amphipod species. We use several methods to define molecular operational taxonomic units (MOTUs), based on two mitochondrial markers (COI and 16S), and assess their congruence with current species-level taxonomy based on morphology. Depending on the method, we find that 54–69% of species had congruent morpho-molecular boundaries. The cases of incongruence resulted from lumping distinct morphospecies into a single MOTU (7–27%), splitting a morphospecies into several MOTUs (4–28%), or both (4–11%). MOTUs defined by distance-based methods without a priori divergence thresholds showed the highest congruence with morphological taxonomy. These results indicate that DNA barcoding is valuable for clarifying the diversity of Ponto-Caspian amphipods, but reveals that extensive work is needed to resolve taxonomic uncertainties. Our study advances the DNA barcode reference library for the European aquatic biota, paving the way towards improved taxonomic knowledge needed to enhance monitoring and conservation efforts.https://doi.org/10.1038/s41598-022-15442-w |
| spellingShingle | Denis Copilaş-Ciocianu Tomasz Rewicz Arthur F. Sands Dmitry Palatov Ivan Marin Kęstutis Arbačiauskas Paul D. N. Hebert Michal Grabowski Asta Audzijonyte A DNA barcode reference library for endemic Ponto-Caspian amphipods Scientific Reports |
| title | A DNA barcode reference library for endemic Ponto-Caspian amphipods |
| title_full | A DNA barcode reference library for endemic Ponto-Caspian amphipods |
| title_fullStr | A DNA barcode reference library for endemic Ponto-Caspian amphipods |
| title_full_unstemmed | A DNA barcode reference library for endemic Ponto-Caspian amphipods |
| title_short | A DNA barcode reference library for endemic Ponto-Caspian amphipods |
| title_sort | dna barcode reference library for endemic ponto caspian amphipods |
| url | https://doi.org/10.1038/s41598-022-15442-w |
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