Robust and persistent SARS-CoV-2 infection in the human intestinal brush border expressing cells
ABSTRACTStudies on patients with the coronavirus disease-2019 (COVID-19) have implicated that the gastrointestinal (GI) tract is a major site of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We established a human GI tract cell line model highly permissive to SARS-CoV-2. Th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1827985 |
| _version_ | 1797265782157082624 |
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| author | Sunhee Lee Gun Young Yoon Jinjong Myoung Seong-Jun Kim Dae-Gyun Ahn |
| author_facet | Sunhee Lee Gun Young Yoon Jinjong Myoung Seong-Jun Kim Dae-Gyun Ahn |
| author_sort | Sunhee Lee |
| collection | DOAJ |
| description | ABSTRACTStudies on patients with the coronavirus disease-2019 (COVID-19) have implicated that the gastrointestinal (GI) tract is a major site of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We established a human GI tract cell line model highly permissive to SARS-CoV-2. These cells, C2BBe1 intestinal cells with a brush border having high levels of transmembrane serine protease 2 (TMPRSS2), showed robust viral propagation, and could be persistently infected with SARS-CoV-2, supporting the clinical observations of persistent GI infection in COVID-19 patients. Ectopic expression of viral receptors revealed that the levels of angiotensin-converting enzyme 2 (ACE2) expression confer permissiveness to SARS-CoV-2 infection, and TMPRSS2 greatly facilitates ACE2-mediated SARS-CoV-2 dissemination. Interestingly, ACE2 but not TMPRSS2 expression was significantly promoted by enterocytic differentiation, suggesting that the state of enterocytic differentiation may serve as a determining factor for viral propagation. Thus, our study sheds light on the pathogenesis of SARS-CoV-2 in the GI tract. |
| first_indexed | 2024-03-07T17:23:44Z |
| format | Article |
| id | doaj.art-1546b938aaab4391bd652ae16478e528 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-25T00:50:16Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-1546b938aaab4391bd652ae16478e5282024-03-11T16:04:25ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01912169217910.1080/22221751.2020.1827985Robust and persistent SARS-CoV-2 infection in the human intestinal brush border expressing cellsSunhee Lee0Gun Young Yoon1Jinjong Myoung2Seong-Jun Kim3Dae-Gyun Ahn4Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, South KoreaCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, South KoreaKorea Zoonosis Research Institute & Genetic Engineering Research Institute, Jeonbuk National University, Jeollabuk-do, South KoreaCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, South KoreaCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, South KoreaABSTRACTStudies on patients with the coronavirus disease-2019 (COVID-19) have implicated that the gastrointestinal (GI) tract is a major site of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We established a human GI tract cell line model highly permissive to SARS-CoV-2. These cells, C2BBe1 intestinal cells with a brush border having high levels of transmembrane serine protease 2 (TMPRSS2), showed robust viral propagation, and could be persistently infected with SARS-CoV-2, supporting the clinical observations of persistent GI infection in COVID-19 patients. Ectopic expression of viral receptors revealed that the levels of angiotensin-converting enzyme 2 (ACE2) expression confer permissiveness to SARS-CoV-2 infection, and TMPRSS2 greatly facilitates ACE2-mediated SARS-CoV-2 dissemination. Interestingly, ACE2 but not TMPRSS2 expression was significantly promoted by enterocytic differentiation, suggesting that the state of enterocytic differentiation may serve as a determining factor for viral propagation. Thus, our study sheds light on the pathogenesis of SARS-CoV-2 in the GI tract.https://www.tandfonline.com/doi/10.1080/22221751.2020.1827985COVID-19SARS-CoV-2coronaviruspersistentACE2 |
| spellingShingle | Sunhee Lee Gun Young Yoon Jinjong Myoung Seong-Jun Kim Dae-Gyun Ahn Robust and persistent SARS-CoV-2 infection in the human intestinal brush border expressing cells Emerging Microbes and Infections COVID-19 SARS-CoV-2 coronavirus persistent ACE2 |
| title | Robust and persistent SARS-CoV-2 infection in the human intestinal brush border expressing cells |
| title_full | Robust and persistent SARS-CoV-2 infection in the human intestinal brush border expressing cells |
| title_fullStr | Robust and persistent SARS-CoV-2 infection in the human intestinal brush border expressing cells |
| title_full_unstemmed | Robust and persistent SARS-CoV-2 infection in the human intestinal brush border expressing cells |
| title_short | Robust and persistent SARS-CoV-2 infection in the human intestinal brush border expressing cells |
| title_sort | robust and persistent sars cov 2 infection in the human intestinal brush border expressing cells |
| topic | COVID-19 SARS-CoV-2 coronavirus persistent ACE2 |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1827985 |
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