Exploring the Toxic Effects of ZEA on IPEC-J2 Cells from the Inflammatory Response and Apoptosis
Zearalenone (ZEA) is the most common fungal toxin contaminating livestock and poultry feeding, especially in pigs, causing severe toxic effects and economic losses. However, the mechanism of ZEA damage to the intestine is unknown. We constructed an in vitro model of ZEA toxicity in a porcine small i...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/17/2731 |
| _version_ | 1797582917954699264 |
|---|---|
| author | Haoyue Guan Wenxue Ma Qiong Wu Jingzeng Cai Ziwei Zhang |
| author_facet | Haoyue Guan Wenxue Ma Qiong Wu Jingzeng Cai Ziwei Zhang |
| author_sort | Haoyue Guan |
| collection | DOAJ |
| description | Zearalenone (ZEA) is the most common fungal toxin contaminating livestock and poultry feeding, especially in pigs, causing severe toxic effects and economic losses. However, the mechanism of ZEA damage to the intestine is unknown. We constructed an in vitro model of ZEA toxicity in a porcine small intestinal epithelial cell (IPEC-J2) line. ZEA causes severe oxidative stress in porcine small intestine cells, such as the production of ROS and a significant decrease in the levels of antioxidant enzymes GSH, CAT, SOD, and T-AOC. ZEA also caused apoptosis in porcine small intestine cells, resulting in a significant reduction in protein and/or mRNA expression of apoptosis-related pathway factors such as P53, caspase 3, caspase 9, Bax, and Cyt-c, which in turn caused a significant decrease in protein and/or mRNA expression of inflammatory-related factors such as IL-1β, IL-2, Cox-2, NF-κD, NLRP3, IL-6, and IL -18, which in turn caused a significant increase in protein and/or mRNA expression levels. The final results suggest that ZEA can cause a severe toxic response in porcine small intestine cells, with oxidative stress, apoptotic cell death and inflammatory damage. |
| first_indexed | 2024-03-10T23:28:22Z |
| format | Article |
| id | doaj.art-1547a6929ae54af9bd8d4785628a6243 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T23:28:22Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-1547a6929ae54af9bd8d4785628a62432023-11-19T07:46:36ZengMDPI AGAnimals2076-26152023-08-011317273110.3390/ani13172731Exploring the Toxic Effects of ZEA on IPEC-J2 Cells from the Inflammatory Response and ApoptosisHaoyue Guan0Wenxue Ma1Qiong Wu2Jingzeng Cai3Ziwei Zhang4College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaAnimal Science and Technology College, Beijing University of Agriculture, Beijing 100096, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaZearalenone (ZEA) is the most common fungal toxin contaminating livestock and poultry feeding, especially in pigs, causing severe toxic effects and economic losses. However, the mechanism of ZEA damage to the intestine is unknown. We constructed an in vitro model of ZEA toxicity in a porcine small intestinal epithelial cell (IPEC-J2) line. ZEA causes severe oxidative stress in porcine small intestine cells, such as the production of ROS and a significant decrease in the levels of antioxidant enzymes GSH, CAT, SOD, and T-AOC. ZEA also caused apoptosis in porcine small intestine cells, resulting in a significant reduction in protein and/or mRNA expression of apoptosis-related pathway factors such as P53, caspase 3, caspase 9, Bax, and Cyt-c, which in turn caused a significant decrease in protein and/or mRNA expression of inflammatory-related factors such as IL-1β, IL-2, Cox-2, NF-κD, NLRP3, IL-6, and IL -18, which in turn caused a significant increase in protein and/or mRNA expression levels. The final results suggest that ZEA can cause a severe toxic response in porcine small intestine cells, with oxidative stress, apoptotic cell death and inflammatory damage.https://www.mdpi.com/2076-2615/13/17/2731zearalenoneIPEC-J2 cellsoxidative stressapoptosisinflammatory factors |
| spellingShingle | Haoyue Guan Wenxue Ma Qiong Wu Jingzeng Cai Ziwei Zhang Exploring the Toxic Effects of ZEA on IPEC-J2 Cells from the Inflammatory Response and Apoptosis Animals zearalenone IPEC-J2 cells oxidative stress apoptosis inflammatory factors |
| title | Exploring the Toxic Effects of ZEA on IPEC-J2 Cells from the Inflammatory Response and Apoptosis |
| title_full | Exploring the Toxic Effects of ZEA on IPEC-J2 Cells from the Inflammatory Response and Apoptosis |
| title_fullStr | Exploring the Toxic Effects of ZEA on IPEC-J2 Cells from the Inflammatory Response and Apoptosis |
| title_full_unstemmed | Exploring the Toxic Effects of ZEA on IPEC-J2 Cells from the Inflammatory Response and Apoptosis |
| title_short | Exploring the Toxic Effects of ZEA on IPEC-J2 Cells from the Inflammatory Response and Apoptosis |
| title_sort | exploring the toxic effects of zea on ipec j2 cells from the inflammatory response and apoptosis |
| topic | zearalenone IPEC-J2 cells oxidative stress apoptosis inflammatory factors |
| url | https://www.mdpi.com/2076-2615/13/17/2731 |
| work_keys_str_mv | AT haoyueguan exploringthetoxiceffectsofzeaonipecj2cellsfromtheinflammatoryresponseandapoptosis AT wenxuema exploringthetoxiceffectsofzeaonipecj2cellsfromtheinflammatoryresponseandapoptosis AT qiongwu exploringthetoxiceffectsofzeaonipecj2cellsfromtheinflammatoryresponseandapoptosis AT jingzengcai exploringthetoxiceffectsofzeaonipecj2cellsfromtheinflammatoryresponseandapoptosis AT ziweizhang exploringthetoxiceffectsofzeaonipecj2cellsfromtheinflammatoryresponseandapoptosis |