QuEChERS-Based Approach to the Extraction of Five Calcium Channel Blockers from Plasma Determined by UPLC-MS/MS
Here, a QuEChERS (quick, easy, cheap, effective, rugged, and safe) pretreatment method was combined with UPLC-MS/MS to facilitate the rapid and reliable simultaneous detection of five calcium channel blockers (CCBs) in human plasma. For this approach, samples were treated with 1 mL of acetonitrile,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/2/671 |
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| author | Tingting Zhao Wen Jiang Xiaolan Zhen Chengcheng Jin Yifan Zhang Hui Li |
| author_facet | Tingting Zhao Wen Jiang Xiaolan Zhen Chengcheng Jin Yifan Zhang Hui Li |
| author_sort | Tingting Zhao |
| collection | DOAJ |
| description | Here, a QuEChERS (quick, easy, cheap, effective, rugged, and safe) pretreatment method was combined with UPLC-MS/MS to facilitate the rapid and reliable simultaneous detection of five calcium channel blockers (CCBs) in human plasma. For this approach, samples were treated with 1 mL of acetonitrile, 350 mg of magnesium sulfate, and 70 mg of PSA adsorbent prior to centrifugation. Supernatants then underwent gradient elution for 8 min with an Agilent C18 column using an acetonitrile-water solution supplemented with 5 mmol⋅L<sup>−1</sup> of ammonium acetate. This technique exhibited a good linear response in the 1–800 ng⋅mL<sup>−1</sup> range for the analyzed drugs, with an R<sup>2</sup>≥ 0.9921, an accuracy of 87.54–113.05%, a matrix effect (ME) of 91.21–116.39%, a precision of 0.19–11.64%, and stability of no more than 10.05%. This time-saving QuEChERS reagent-based pretreatment technique thus allowed for the simultaneous and accurate detection of five CCBs in human plasma samples, providing a promising new basis for therapeutic drug monitoring in patients with hypertension. |
| first_indexed | 2024-03-09T11:35:36Z |
| format | Article |
| id | doaj.art-154e4db3b8834ad7b384dc2f4d1fa466 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T11:35:36Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-154e4db3b8834ad7b384dc2f4d1fa4662023-11-30T23:43:07ZengMDPI AGMolecules1420-30492023-01-0128267110.3390/molecules28020671QuEChERS-Based Approach to the Extraction of Five Calcium Channel Blockers from Plasma Determined by UPLC-MS/MSTingting Zhao0Wen Jiang1Xiaolan Zhen2Chengcheng Jin3Yifan Zhang4Hui Li5College of Pharmacy, Hebei Medical University, Shijiazhuang 050000, ChinaCollege of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050000, ChinaHebei Institute of Drug and Medical Device Inspection, Shijiazhuang 050000, ChinaCollege of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050000, ChinaCollege of Pharmacy, Hebei Medical University, Shijiazhuang 050000, ChinaCollege of Pharmacy, Hebei Medical University, Shijiazhuang 050000, ChinaHere, a QuEChERS (quick, easy, cheap, effective, rugged, and safe) pretreatment method was combined with UPLC-MS/MS to facilitate the rapid and reliable simultaneous detection of five calcium channel blockers (CCBs) in human plasma. For this approach, samples were treated with 1 mL of acetonitrile, 350 mg of magnesium sulfate, and 70 mg of PSA adsorbent prior to centrifugation. Supernatants then underwent gradient elution for 8 min with an Agilent C18 column using an acetonitrile-water solution supplemented with 5 mmol⋅L<sup>−1</sup> of ammonium acetate. This technique exhibited a good linear response in the 1–800 ng⋅mL<sup>−1</sup> range for the analyzed drugs, with an R<sup>2</sup>≥ 0.9921, an accuracy of 87.54–113.05%, a matrix effect (ME) of 91.21–116.39%, a precision of 0.19–11.64%, and stability of no more than 10.05%. This time-saving QuEChERS reagent-based pretreatment technique thus allowed for the simultaneous and accurate detection of five CCBs in human plasma samples, providing a promising new basis for therapeutic drug monitoring in patients with hypertension.https://www.mdpi.com/1420-3049/28/2/671calcium channel blockersQuEChERSUPLC-MS/MShuman plasmahypertension |
| spellingShingle | Tingting Zhao Wen Jiang Xiaolan Zhen Chengcheng Jin Yifan Zhang Hui Li QuEChERS-Based Approach to the Extraction of Five Calcium Channel Blockers from Plasma Determined by UPLC-MS/MS Molecules calcium channel blockers QuEChERS UPLC-MS/MS human plasma hypertension |
| title | QuEChERS-Based Approach to the Extraction of Five Calcium Channel Blockers from Plasma Determined by UPLC-MS/MS |
| title_full | QuEChERS-Based Approach to the Extraction of Five Calcium Channel Blockers from Plasma Determined by UPLC-MS/MS |
| title_fullStr | QuEChERS-Based Approach to the Extraction of Five Calcium Channel Blockers from Plasma Determined by UPLC-MS/MS |
| title_full_unstemmed | QuEChERS-Based Approach to the Extraction of Five Calcium Channel Blockers from Plasma Determined by UPLC-MS/MS |
| title_short | QuEChERS-Based Approach to the Extraction of Five Calcium Channel Blockers from Plasma Determined by UPLC-MS/MS |
| title_sort | quechers based approach to the extraction of five calcium channel blockers from plasma determined by uplc ms ms |
| topic | calcium channel blockers QuEChERS UPLC-MS/MS human plasma hypertension |
| url | https://www.mdpi.com/1420-3049/28/2/671 |
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