Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilms
Much is known regarding the antibiotic susceptibility of planktonic cultures of Mycobacterium tuberculosis, the bacterium responsible for the lung disease tuberculosis (TB). As planktonically-grown M. tuberculosis are unlikely to be entirely representative of the bacterium during infection, we set o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2016-11-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/2717.pdf |
| _version_ | 1797418848668876800 |
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| author | James P. Dalton Benedict Uy Narisa Phummarin Brent R. Copp William A. Denny Simon Swift Siouxsie Wiles |
| author_facet | James P. Dalton Benedict Uy Narisa Phummarin Brent R. Copp William A. Denny Simon Swift Siouxsie Wiles |
| author_sort | James P. Dalton |
| collection | DOAJ |
| description | Much is known regarding the antibiotic susceptibility of planktonic cultures of Mycobacterium tuberculosis, the bacterium responsible for the lung disease tuberculosis (TB). As planktonically-grown M. tuberculosis are unlikely to be entirely representative of the bacterium during infection, we set out to determine how effective a range of anti-mycobacterial treatments were against M. tuberculosis growing as a biofilm, a bacterial phenotype known to be more resistant to antibiotic treatment. Light levels from bioluminescently-labelled M. tuberculosis H37Rv (strain BSG001) were used as a surrogate for bacterial viability, and were monitored before and after one week of treatment. After treatment, biofilms were disrupted, washed and inoculated into fresh broth and plated onto solid media to rescue any surviving bacteria. We found that in this phenotypic state M. tuberculosis was resistant to the majority of the compounds tested. Minimum inhibitory concentrations (MICs) increased by 20-fold to greater than 1,000-fold, underlying the potential of this phenotype to cause significant problems during treatment. |
| first_indexed | 2024-03-09T06:38:54Z |
| format | Article |
| id | doaj.art-1550deb607ba4325ba3511510b8c1091 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:38:54Z |
| publishDate | 2016-11-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-1550deb607ba4325ba3511510b8c10912023-12-03T10:53:34ZengPeerJ Inc.PeerJ2167-83592016-11-014e271710.7717/peerj.2717Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilmsJames P. Dalton0Benedict Uy1Narisa Phummarin2Brent R. Copp3William A. Denny4Simon Swift5Siouxsie Wiles6Molecular Medicine and Pathology, University of Auckland, Auckland, New ZealandMolecular Medicine and Pathology, University of Auckland, Auckland, New ZealandSchool of Chemical Sciences, University of Auckland, Auckland, New ZealandMaurice Wilkins Centre for Molecular Biodiscovery, Auckland, New ZealandMaurice Wilkins Centre for Molecular Biodiscovery, Auckland, New ZealandMolecular Medicine and Pathology, University of Auckland, Auckland, New ZealandMolecular Medicine and Pathology, University of Auckland, Auckland, New ZealandMuch is known regarding the antibiotic susceptibility of planktonic cultures of Mycobacterium tuberculosis, the bacterium responsible for the lung disease tuberculosis (TB). As planktonically-grown M. tuberculosis are unlikely to be entirely representative of the bacterium during infection, we set out to determine how effective a range of anti-mycobacterial treatments were against M. tuberculosis growing as a biofilm, a bacterial phenotype known to be more resistant to antibiotic treatment. Light levels from bioluminescently-labelled M. tuberculosis H37Rv (strain BSG001) were used as a surrogate for bacterial viability, and were monitored before and after one week of treatment. After treatment, biofilms were disrupted, washed and inoculated into fresh broth and plated onto solid media to rescue any surviving bacteria. We found that in this phenotypic state M. tuberculosis was resistant to the majority of the compounds tested. Minimum inhibitory concentrations (MICs) increased by 20-fold to greater than 1,000-fold, underlying the potential of this phenotype to cause significant problems during treatment.https://peerj.com/articles/2717.pdfMycobacterium tuberculosisMTBTBBiofilmPellicleAscorbic acid |
| spellingShingle | James P. Dalton Benedict Uy Narisa Phummarin Brent R. Copp William A. Denny Simon Swift Siouxsie Wiles Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilms PeerJ Mycobacterium tuberculosis MTB TB Biofilm Pellicle Ascorbic acid |
| title | Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilms |
| title_full | Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilms |
| title_fullStr | Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilms |
| title_full_unstemmed | Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilms |
| title_short | Effect of common and experimental anti-tuberculosis treatments on Mycobacterium tuberculosis growing as biofilms |
| title_sort | effect of common and experimental anti tuberculosis treatments on mycobacterium tuberculosis growing as biofilms |
| topic | Mycobacterium tuberculosis MTB TB Biofilm Pellicle Ascorbic acid |
| url | https://peerj.com/articles/2717.pdf |
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