Effect of Green-biosynthesis Aluminum Nanoparticles (Al NPs) on Salmonella enterica Isolated from Baghdad City
This study is aimed to Green-synthesize and characterize Al NPs from Clove (Syzygium aromaticum L.) buds plant extract and to investigate their effect on isolated and characterized Salmonella enterica growth. S. aromaticum buds aqueous extract was prepared from local market clove, then mixed with...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2023-10-01
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| Series: | Baghdad Science Journal |
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| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7526 |
| _version_ | 1797647664780673024 |
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| author | Zahraa A. AlShaheeb Asma G. Oraibi Zaid A. Thabit |
| author_facet | Zahraa A. AlShaheeb Asma G. Oraibi Zaid A. Thabit |
| author_sort | Zahraa A. AlShaheeb |
| collection | DOAJ |
| description |
This study is aimed to Green-synthesize and characterize Al NPs from Clove (Syzygium aromaticum
L.) buds plant extract and to investigate their effect on isolated and characterized Salmonella enterica growth.
S. aromaticum buds aqueous extract was prepared from local market clove, then mixed with Aluminum nitrate
Al(NO3)3. 9 H2O, 99.9% in ¼ ratio for green-synthesizing of Al NPs. Color change was a primary confirmation
of Al NPs biosynthesis. The biosynthesized nanoparticles were identified and characterized by AFM, SEM,
EDX and UV–Visible spectrophotometer. AFM data recorded 122nm particles size and the surface roughness
RMs) of the pure S. aromaticum buds aqueous extract recorded 17.5nm particles size, while the results of Al
NPs in the tested sample recorded 21nm particles size with surface roughness RMs about 2.35nm. SEM images
revealed the presence of Al NPs with diameters ranged from 33.5-70.4nm with regular spiracles shape particles
in the prepared biosynthesized nanoparticle sample. The EDX spectrum analysis showed that the Aluminium
weight ratio was 1.75, while it was 50.498 in the Al NPs sample prepared from aqueous extract. UV-Visible
spectroscopy data revealed that biosynthesized Al NPs were absorbed at 213nm while Aluminum nitrate was
absorbed at 258nm. These results indicate the formation of Al NPs. The antibacterial activity showed that Al
NPs exhibited having high antibacterial activity on Salmonella spp. isolates compared to the effect of the
control agent (imipenem) in this experiment. We conclude that biosynthesized Al NPs from clove aqueous
extract can be exploited as natural antibacterial compounds to inhibit the growth of Salmonella.
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| first_indexed | 2024-03-11T15:19:48Z |
| format | Article |
| id | doaj.art-1551595ea047495aa1bd4cc8d8b88e3a |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-03-11T15:19:48Z |
| publishDate | 2023-10-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-1551595ea047495aa1bd4cc8d8b88e3a2023-10-28T19:58:12ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862023-10-01205(Suppl.)10.21123/bsj.2023.7526Effect of Green-biosynthesis Aluminum Nanoparticles (Al NPs) on Salmonella enterica Isolated from Baghdad CityZahraa A. AlShaheeb0Asma G. Oraibi1Zaid A. Thabit2College of Biotechnology, Al-Nahrain University, Baghdad, Iraq.College of Biotechnology, Al-Nahrain University, Baghdad, Iraq.Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq. This study is aimed to Green-synthesize and characterize Al NPs from Clove (Syzygium aromaticum L.) buds plant extract and to investigate their effect on isolated and characterized Salmonella enterica growth. S. aromaticum buds aqueous extract was prepared from local market clove, then mixed with Aluminum nitrate Al(NO3)3. 9 H2O, 99.9% in ¼ ratio for green-synthesizing of Al NPs. Color change was a primary confirmation of Al NPs biosynthesis. The biosynthesized nanoparticles were identified and characterized by AFM, SEM, EDX and UV–Visible spectrophotometer. AFM data recorded 122nm particles size and the surface roughness RMs) of the pure S. aromaticum buds aqueous extract recorded 17.5nm particles size, while the results of Al NPs in the tested sample recorded 21nm particles size with surface roughness RMs about 2.35nm. SEM images revealed the presence of Al NPs with diameters ranged from 33.5-70.4nm with regular spiracles shape particles in the prepared biosynthesized nanoparticle sample. The EDX spectrum analysis showed that the Aluminium weight ratio was 1.75, while it was 50.498 in the Al NPs sample prepared from aqueous extract. UV-Visible spectroscopy data revealed that biosynthesized Al NPs were absorbed at 213nm while Aluminum nitrate was absorbed at 258nm. These results indicate the formation of Al NPs. The antibacterial activity showed that Al NPs exhibited having high antibacterial activity on Salmonella spp. isolates compared to the effect of the control agent (imipenem) in this experiment. We conclude that biosynthesized Al NPs from clove aqueous extract can be exploited as natural antibacterial compounds to inhibit the growth of Salmonella. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7526Al NPs, Biosynthesized Al NPs, Antimicrobial effect of Al NPs, Foodborne disease, Salmonellosis. |
| spellingShingle | Zahraa A. AlShaheeb Asma G. Oraibi Zaid A. Thabit Effect of Green-biosynthesis Aluminum Nanoparticles (Al NPs) on Salmonella enterica Isolated from Baghdad City Baghdad Science Journal Al NPs, Biosynthesized Al NPs, Antimicrobial effect of Al NPs, Foodborne disease, Salmonellosis. |
| title | Effect of Green-biosynthesis Aluminum Nanoparticles (Al NPs) on Salmonella enterica Isolated from Baghdad City |
| title_full | Effect of Green-biosynthesis Aluminum Nanoparticles (Al NPs) on Salmonella enterica Isolated from Baghdad City |
| title_fullStr | Effect of Green-biosynthesis Aluminum Nanoparticles (Al NPs) on Salmonella enterica Isolated from Baghdad City |
| title_full_unstemmed | Effect of Green-biosynthesis Aluminum Nanoparticles (Al NPs) on Salmonella enterica Isolated from Baghdad City |
| title_short | Effect of Green-biosynthesis Aluminum Nanoparticles (Al NPs) on Salmonella enterica Isolated from Baghdad City |
| title_sort | effect of green biosynthesis aluminum nanoparticles al nps on salmonella enterica isolated from baghdad city |
| topic | Al NPs, Biosynthesized Al NPs, Antimicrobial effect of Al NPs, Foodborne disease, Salmonellosis. |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7526 |
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