Characteristics of Planococcus antioxidans sp. nov., an antioxidant‐producing strain isolated from the desert soil in the Qinghai–Tibetan Plateau
Abstract Strain Y74T was an isolate from the sandy soil in the town of Huatugou, Qinghai–Tibet Plateau, China. An analysis of this strain's phenotypic, chemotaxonomic, and genomic characteristics established the relationship of the isolate with the genus Planococcus. Strain Y74T was able to gro...
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| Format: | Article |
| Language: | English |
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Wiley
2020-06-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.1028 |
| _version_ | 1818503618081325056 |
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| author | Binglin Zhang Ruiqi Yang Gaosen Zhang Yang Liu Dongming Zhang Wei Zhang Tuo Chen Guangxiu Liu |
| author_facet | Binglin Zhang Ruiqi Yang Gaosen Zhang Yang Liu Dongming Zhang Wei Zhang Tuo Chen Guangxiu Liu |
| author_sort | Binglin Zhang |
| collection | DOAJ |
| description | Abstract Strain Y74T was an isolate from the sandy soil in the town of Huatugou, Qinghai–Tibet Plateau, China. An analysis of this strain's phenotypic, chemotaxonomic, and genomic characteristics established the relationship of the isolate with the genus Planococcus. Strain Y74T was able to grow between 4 and 42°C (with an optimum temperature of 28°C) at pH values of 6–8.5 and in 0%–7% (w/v) NaCl. The dominant quinones were MK‐8 and MK‐7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The majority of the fatty acid content was anteiso‐C15:0 (28.8%) followed by C16:1 ω7c alcohol (20.9%) and iso‐C14:0 (13.4%). The 16S rRNA gene sequence similarity analysis demonstrated a stable branch formed by strain Y74T and Planococcus halotolerans SCU63T (99.66%). The digital DNA–DNA hybridization between these two strains was 57.2%. The G + C content in the DNA of Y74T was 44.5 mol%. In addition, the morphological, physiological, and chemotaxonomic pattern clearly differentiated the isolates from their known relatives. In conclusion, the strain Y74T (=JCM 32826T = CICC24461T) represents a novel member of the genus Planococcus, for which the name Planococcus antioxidans sp. nov. is proposed. Strain Y74T was found to have potent antioxidant activity via its hydrogen peroxide tolerance and its 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical‐scavenging activity. The DPPH radical‐scavenging activity was determined to be 40.2 ± 0.7%. The genomic analysis indicated that six peroxidases genes, one superoxide dismutase gene, and one dprA (DNA‐protecting protein) are present in the genome of Y74T. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-10T21:26:20Z |
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| spelling | doaj.art-1552554cf29946de8edfc9c2b48235f72022-12-22T01:32:58ZengWileyMicrobiologyOpen2045-88272020-06-01961183119610.1002/mbo3.1028Characteristics of Planococcus antioxidans sp. nov., an antioxidant‐producing strain isolated from the desert soil in the Qinghai–Tibetan PlateauBinglin Zhang0Ruiqi Yang1Gaosen Zhang2Yang Liu3Dongming Zhang4Wei Zhang5Tuo Chen6Guangxiu Liu7State Key Laboratory of Cryospheric Sciences Northwest Institute of Eco‐Environment and Resources Chinese Academy of Sciences Lanzhou ChinaCollege of Geography and Environmental Engineering Lanzhou City University Lanzhou ChinaKey Laboratory of Extreme Environmental Microbial Resources and Engineering Lanzhou ChinaKey Laboratory of Extreme Environmental Microbial Resources and Engineering Lanzhou ChinaKey Laboratory of Extreme Environmental Microbial Resources and Engineering Lanzhou ChinaKey Laboratory of Extreme Environmental Microbial Resources and Engineering Lanzhou ChinaState Key Laboratory of Cryospheric Sciences Northwest Institute of Eco‐Environment and Resources Chinese Academy of Sciences Lanzhou ChinaKey Laboratory of Extreme Environmental Microbial Resources and Engineering Lanzhou ChinaAbstract Strain Y74T was an isolate from the sandy soil in the town of Huatugou, Qinghai–Tibet Plateau, China. An analysis of this strain's phenotypic, chemotaxonomic, and genomic characteristics established the relationship of the isolate with the genus Planococcus. Strain Y74T was able to grow between 4 and 42°C (with an optimum temperature of 28°C) at pH values of 6–8.5 and in 0%–7% (w/v) NaCl. The dominant quinones were MK‐8 and MK‐7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The majority of the fatty acid content was anteiso‐C15:0 (28.8%) followed by C16:1 ω7c alcohol (20.9%) and iso‐C14:0 (13.4%). The 16S rRNA gene sequence similarity analysis demonstrated a stable branch formed by strain Y74T and Planococcus halotolerans SCU63T (99.66%). The digital DNA–DNA hybridization between these two strains was 57.2%. The G + C content in the DNA of Y74T was 44.5 mol%. In addition, the morphological, physiological, and chemotaxonomic pattern clearly differentiated the isolates from their known relatives. In conclusion, the strain Y74T (=JCM 32826T = CICC24461T) represents a novel member of the genus Planococcus, for which the name Planococcus antioxidans sp. nov. is proposed. Strain Y74T was found to have potent antioxidant activity via its hydrogen peroxide tolerance and its 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical‐scavenging activity. The DPPH radical‐scavenging activity was determined to be 40.2 ± 0.7%. The genomic analysis indicated that six peroxidases genes, one superoxide dismutase gene, and one dprA (DNA‐protecting protein) are present in the genome of Y74T.https://doi.org/10.1002/mbo3.1028antioxidantPlanococcus antioxidanspolyphasic taxonomyQinghai–Tibetan Plateau |
| spellingShingle | Binglin Zhang Ruiqi Yang Gaosen Zhang Yang Liu Dongming Zhang Wei Zhang Tuo Chen Guangxiu Liu Characteristics of Planococcus antioxidans sp. nov., an antioxidant‐producing strain isolated from the desert soil in the Qinghai–Tibetan Plateau MicrobiologyOpen antioxidant Planococcus antioxidans polyphasic taxonomy Qinghai–Tibetan Plateau |
| title | Characteristics of Planococcus antioxidans sp. nov., an antioxidant‐producing strain isolated from the desert soil in the Qinghai–Tibetan Plateau |
| title_full | Characteristics of Planococcus antioxidans sp. nov., an antioxidant‐producing strain isolated from the desert soil in the Qinghai–Tibetan Plateau |
| title_fullStr | Characteristics of Planococcus antioxidans sp. nov., an antioxidant‐producing strain isolated from the desert soil in the Qinghai–Tibetan Plateau |
| title_full_unstemmed | Characteristics of Planococcus antioxidans sp. nov., an antioxidant‐producing strain isolated from the desert soil in the Qinghai–Tibetan Plateau |
| title_short | Characteristics of Planococcus antioxidans sp. nov., an antioxidant‐producing strain isolated from the desert soil in the Qinghai–Tibetan Plateau |
| title_sort | characteristics of planococcus antioxidans sp nov an antioxidant producing strain isolated from the desert soil in the qinghai tibetan plateau |
| topic | antioxidant Planococcus antioxidans polyphasic taxonomy Qinghai–Tibetan Plateau |
| url | https://doi.org/10.1002/mbo3.1028 |
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