Effects of Surface Modification on Adsorption Behavior of Cell and Protein on Titanium Surface by Using Quartz Crystal Microbalance System
Primary stability and osseointegration are major challenges in dental implant treatments, where the material surface properties and wettability are critical in the early formation of hard tissue around the implant. In this study, a quartz crystal microbalance (QCM) was used to measure the nanogram l...
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MDPI AG
2020-12-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/14/1/97 |
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| author | Takumi Matsumoto Yuichiro Tashiro Satoshi Komasa Akiko Miyake Yutaka Komasa Joji Okazaki |
| author_facet | Takumi Matsumoto Yuichiro Tashiro Satoshi Komasa Akiko Miyake Yutaka Komasa Joji Okazaki |
| author_sort | Takumi Matsumoto |
| collection | DOAJ |
| description | Primary stability and osseointegration are major challenges in dental implant treatments, where the material surface properties and wettability are critical in the early formation of hard tissue around the implant. In this study, a quartz crystal microbalance (QCM) was used to measure the nanogram level amount of protein and bone marrow cells adhered to the surfaces of titanium (Ti) surface in real time. The effects of ultraviolet (UV) and atmospheric-pressure plasma treatment to impart surface hydrophilicity to the implant surface were evaluated. The surface treatment methods resulted in a marked decrease in the surface carbon (C) content and increase in the oxygen (O) content, along with super hydrophilicity. The results of QCM measurements showed that adhesion of both adhesive proteins and bone marrow cells was enhanced after surface treatment. Although both methods produced implants with good osseointegration behavior and less reactive oxidative species, the samples treated with atmospheric pressure plasma showed the best overall performance and are recommended for clinical use. It was verified that QCM is an effective method for analyzing the initial adhesion process on dental implants. |
| first_indexed | 2024-03-10T13:42:17Z |
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| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T13:42:17Z |
| publishDate | 2020-12-01 |
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| spelling | doaj.art-15545567df6545068c73f7b4fa7c9d912023-11-21T02:51:53ZengMDPI AGMaterials1996-19442020-12-011419710.3390/ma14010097Effects of Surface Modification on Adsorption Behavior of Cell and Protein on Titanium Surface by Using Quartz Crystal Microbalance SystemTakumi Matsumoto0Yuichiro Tashiro1Satoshi Komasa2Akiko Miyake3Yutaka Komasa4Joji Okazaki5Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanDepartment of Oral Health Engineering Faculty of Health Sciences, Osaka Dental University, 1-4-4, Makino-honmachi, Hirakata-shi, Osaka 573-1121, JapanDepartment of Oral Health Engineering Faculty of Health Sciences, Osaka Dental University, 1-4-4, Makino-honmachi, Hirakata-shi, Osaka 573-1121, JapanDepartment of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, JapanPrimary stability and osseointegration are major challenges in dental implant treatments, where the material surface properties and wettability are critical in the early formation of hard tissue around the implant. In this study, a quartz crystal microbalance (QCM) was used to measure the nanogram level amount of protein and bone marrow cells adhered to the surfaces of titanium (Ti) surface in real time. The effects of ultraviolet (UV) and atmospheric-pressure plasma treatment to impart surface hydrophilicity to the implant surface were evaluated. The surface treatment methods resulted in a marked decrease in the surface carbon (C) content and increase in the oxygen (O) content, along with super hydrophilicity. The results of QCM measurements showed that adhesion of both adhesive proteins and bone marrow cells was enhanced after surface treatment. Although both methods produced implants with good osseointegration behavior and less reactive oxidative species, the samples treated with atmospheric pressure plasma showed the best overall performance and are recommended for clinical use. It was verified that QCM is an effective method for analyzing the initial adhesion process on dental implants.https://www.mdpi.com/1996-1944/14/1/97titaniumUV-treatmentatmospheric pressure plasma-treatmentQCM measurementROS assay |
| spellingShingle | Takumi Matsumoto Yuichiro Tashiro Satoshi Komasa Akiko Miyake Yutaka Komasa Joji Okazaki Effects of Surface Modification on Adsorption Behavior of Cell and Protein on Titanium Surface by Using Quartz Crystal Microbalance System Materials titanium UV-treatment atmospheric pressure plasma-treatment QCM measurement ROS assay |
| title | Effects of Surface Modification on Adsorption Behavior of Cell and Protein on Titanium Surface by Using Quartz Crystal Microbalance System |
| title_full | Effects of Surface Modification on Adsorption Behavior of Cell and Protein on Titanium Surface by Using Quartz Crystal Microbalance System |
| title_fullStr | Effects of Surface Modification on Adsorption Behavior of Cell and Protein on Titanium Surface by Using Quartz Crystal Microbalance System |
| title_full_unstemmed | Effects of Surface Modification on Adsorption Behavior of Cell and Protein on Titanium Surface by Using Quartz Crystal Microbalance System |
| title_short | Effects of Surface Modification on Adsorption Behavior of Cell and Protein on Titanium Surface by Using Quartz Crystal Microbalance System |
| title_sort | effects of surface modification on adsorption behavior of cell and protein on titanium surface by using quartz crystal microbalance system |
| topic | titanium UV-treatment atmospheric pressure plasma-treatment QCM measurement ROS assay |
| url | https://www.mdpi.com/1996-1944/14/1/97 |
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