Serological and molecular evidence of hepadnavirus infection in swine
[b]Introduction and objective[/b]. Recently, investigations in a swine herd identified evidence of the existence of a novel member of the Hepadnavirus family endemic in swine. The aim of this study was to investigate the serological and molecular markers of Hepadnavirus circulation in Brazilian dome...
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Institute of Rural Health
2015-02-01
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| Series: | Annals of Agricultural and Environmental Medicine |
| Subjects: | |
| Online Access: | http://journals.indexcopernicus.com/fulltxt.php?ICID=1141361 |
| _version_ | 1818232133777358848 |
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| author | Yasmine R Vieira Marcelle FM Silva Débora RL Santos Antonio A Vieira Janice R Ciacci-Zanella Gonzalo Barquero Bárbara V do Lago Selma A Gomes Marcelo A Pinto Vanessa S de Paula |
| author_facet | Yasmine R Vieira Marcelle FM Silva Débora RL Santos Antonio A Vieira Janice R Ciacci-Zanella Gonzalo Barquero Bárbara V do Lago Selma A Gomes Marcelo A Pinto Vanessa S de Paula |
| author_sort | Yasmine R Vieira |
| collection | DOAJ |
| description | [b]Introduction and objective[/b]. Recently, investigations in a swine herd identified evidence of the existence of a novel member of the Hepadnavirus family endemic in swine. The aim of this study was to investigate the serological and molecular markers of Hepadnavirus circulation in Brazilian domestic swine and wild boar herds, and to evaluate the identity with HBV and other Hepadnaviruses reported previously.
[b]Materials and methods[/b]. For the study, 376 swine were screened for hepatitis B virus serological markers. Analyses were performed in serum samples using commercial enzyme-linked immunosorbent assay (ELISA) kits (DiaSorin®) for anti-HBc, HBsAg and anti-HBs. Reactive and undetermined swine serum samples were selected to perform DNA viral extraction (QIAamp DNA Mini Kit, Qiagen®), partial genome amplification and genome sequencing.
[b]Results[/b]. From 376 swine samples analysed, 28 (7.45%) were reactive to anti-HBc, 3 (0.80%) to HBsAg and 6 (1.6%) to anti-HBs. Besides, more 17 (4.52%) swine samples analyzed were classified in the grey zone of the EIA test to anti-HBc and 2 (0.53%) to HBsAg. From 49 samples molecularly analyzed after serological trial, 4 samples showed a positive result for the qualitative PCR for Hepadnavirus. Phylogenetic reconstruction using partial genome sequencing (360 bp) of 3 samples showed similarity with HBV with 90.8–96.3% of identity.
[b]Conclusions.[/b] Serological and molecular data showed evidence of the circulation of a virus similar to hepatitis B virus in swine. |
| first_indexed | 2024-12-12T11:01:27Z |
| format | Article |
| id | doaj.art-155b5a7031524df5a28505ad2cb2e322 |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-12-12T11:01:27Z |
| publishDate | 2015-02-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-155b5a7031524df5a28505ad2cb2e3222022-12-22T00:26:30ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632015-02-01228773321116Serological and molecular evidence of hepadnavirus infection in swineYasmine R Vieira0Marcelle FM Silva1Débora RL Santos 2Antonio A Vieira3Janice R Ciacci-Zanella 4Gonzalo Barquero 5Bárbara V do Lago 6Selma A Gomes7Marcelo A Pinto8Vanessa S de Paula9Laboratory of Development Technological in Virology, Oswaldo Cruz Institute, FIOCRUZ, RJ, Brazil Laboratory of Comparative and Environmental Virology, Oswaldo Cruz Institute, FIOCRUZ, RJ, BrazilDepartament of Veterinary Microbiology and Immunology, Federal Rural University of Rio De Janeiro, UFRRJ, RJ, BrazilDepartament of Animal Production, Institute of Animal Science, Federal Rural University of Rio De Janeiro, UFRRJ, RJ, BrazilBrazilian National Research Center, EMBRAPA Swine and Poultry, SC, BrazilTropical Sustainability Institute, TSI, SP, Brazil Laboratory of Molecular Virology, Oswaldo Cruz Institute, FIOCRUZ, RJ, BrazilLaboratory of Molecular Virology, Oswaldo Cruz Institute, FIOCRUZ, RJ, BrazilLaboratory of Development Technological in Virology, Oswaldo Cruz Institute, FIOCRUZ, RJ, BrazilLaboratory of Development Technological in Virology, Oswaldo Cruz Institute, FIOCRUZ, RJ, Brazil[b]Introduction and objective[/b]. Recently, investigations in a swine herd identified evidence of the existence of a novel member of the Hepadnavirus family endemic in swine. The aim of this study was to investigate the serological and molecular markers of Hepadnavirus circulation in Brazilian domestic swine and wild boar herds, and to evaluate the identity with HBV and other Hepadnaviruses reported previously. [b]Materials and methods[/b]. For the study, 376 swine were screened for hepatitis B virus serological markers. Analyses were performed in serum samples using commercial enzyme-linked immunosorbent assay (ELISA) kits (DiaSorin®) for anti-HBc, HBsAg and anti-HBs. Reactive and undetermined swine serum samples were selected to perform DNA viral extraction (QIAamp DNA Mini Kit, Qiagen®), partial genome amplification and genome sequencing. [b]Results[/b]. From 376 swine samples analysed, 28 (7.45%) were reactive to anti-HBc, 3 (0.80%) to HBsAg and 6 (1.6%) to anti-HBs. Besides, more 17 (4.52%) swine samples analyzed were classified in the grey zone of the EIA test to anti-HBc and 2 (0.53%) to HBsAg. From 49 samples molecularly analyzed after serological trial, 4 samples showed a positive result for the qualitative PCR for Hepadnavirus. Phylogenetic reconstruction using partial genome sequencing (360 bp) of 3 samples showed similarity with HBV with 90.8–96.3% of identity. [b]Conclusions.[/b] Serological and molecular data showed evidence of the circulation of a virus similar to hepatitis B virus in swine.http://journals.indexcopernicus.com/fulltxt.php?ICID=1141361Swine;Hepadnavirus;diagnostic |
| spellingShingle | Yasmine R Vieira Marcelle FM Silva Débora RL Santos Antonio A Vieira Janice R Ciacci-Zanella Gonzalo Barquero Bárbara V do Lago Selma A Gomes Marcelo A Pinto Vanessa S de Paula Serological and molecular evidence of hepadnavirus infection in swine Annals of Agricultural and Environmental Medicine Swine;Hepadnavirus;diagnostic |
| title | Serological and molecular evidence of hepadnavirus infection in swine |
| title_full | Serological and molecular evidence of hepadnavirus infection in swine |
| title_fullStr | Serological and molecular evidence of hepadnavirus infection in swine |
| title_full_unstemmed | Serological and molecular evidence of hepadnavirus infection in swine |
| title_short | Serological and molecular evidence of hepadnavirus infection in swine |
| title_sort | serological and molecular evidence of hepadnavirus infection in swine |
| topic | Swine;Hepadnavirus;diagnostic |
| url | http://journals.indexcopernicus.com/fulltxt.php?ICID=1141361 |
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