Hydrogen administration improves the growth performance of juvenile largemouth bass (Micropterus salmoides) by increasing feed intake, reducing serum lipids, activating mTOR and Nrf2 signaling pathways, and altering the intestinal microbiota
Hydrogen (H2) gas is recognized for its anti-oxidative, anti-apoptotic, and anti-inflammatory properties in multiple plant and animal models. Nevertheless, there is a lack of research on the effects of H2 administration on fish. This research seeks to investigate how H2 administration affects the gr...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423002880 |
| _version_ | 1827582118465634304 |
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| author | Junru Hu Long Huang Lei Wang Wen Huang Mingjian Lai Xiangni Li Yongchun Lin Yuping Sun |
| author_facet | Junru Hu Long Huang Lei Wang Wen Huang Mingjian Lai Xiangni Li Yongchun Lin Yuping Sun |
| author_sort | Junru Hu |
| collection | DOAJ |
| description | Hydrogen (H2) gas is recognized for its anti-oxidative, anti-apoptotic, and anti-inflammatory properties in multiple plant and animal models. Nevertheless, there is a lack of research on the effects of H2 administration on fish. This research seeks to investigate how H2 administration affects the growth performance, mammalian target of rapamycin (mTOR) and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways, and intestinal microbiota of juvenile largemouth bass under normal feeding conditions. The research randomly allocated 240 fish (3.55 ± 0.01 g) into two treatment groups, each comprising 4 replicate tanks with 30 fish per tank. The control group was maintained in regular water, while the experimental group (referred to as H2 group) was treated with H2-dissolved water for 1 h per day for 6 weeks. Upon analysis of both groups, it was revealed that the weight gain, final weight, feed intake, specific growth rate, and survival rate were significantly improved by H2 treatment (P < 0.05). In addition, administration of H2 significantly reduced the triglyceride content and increased the high-density lipoprotein-cholesterol content in the serum (P < 0.05). Moreover, administration of H2 led to a significant increase in the expression of genes associated with mTOR (PI3K, AKT, TOR, S6K1, and 4EBP1) and Nrf2 (Nrf2, SOD, and GPX) signaling pathways (P < 0.05). Furthermore, H2 significantly increased the expression of growth hormone receptor (GHR) and neuropeptide Y (NPY) genes in the brain of juvenile largemouth bass. H2 did not significantly affect intestinal histology. However, it significantly increased the relative abundance of Sulfuritalea, Bradyrhizobium, and norank_f__Beggiatoaceae (P < 0.05) and reduced the relative abundance of Brevundimonas, unclassified_o__Chlamydiales, and Delftia in the intestinal microbiota (P < 0.05). Thus, these results suggested that providing juvenile largemouth bass with H2-dissolved water for 1 h per day can lead to a significantly increasing in growth performance, stimulating feed intake, activating mTOR and Nrf2 signaling pathways, and augmenting the abundance of beneficial bacteria in the intestines of juvenile largemouth bass. These findings provided a valuable support for the H2 application in the largemouth bass healthy culture. |
| first_indexed | 2024-03-08T22:45:57Z |
| format | Article |
| id | doaj.art-15622627a46a4c8da01b234122273aea |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:45:57Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-15622627a46a4c8da01b234122273aea2023-12-17T06:39:32ZengElsevierAquaculture Reports2352-51342023-12-0133101749Hydrogen administration improves the growth performance of juvenile largemouth bass (Micropterus salmoides) by increasing feed intake, reducing serum lipids, activating mTOR and Nrf2 signaling pathways, and altering the intestinal microbiotaJunru Hu0Long Huang1Lei Wang2Wen Huang3Mingjian Lai4Xiangni Li5Yongchun Lin6Yuping Sun7Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Collaborative Innovation Center of Aquatic Sciences, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaFoshan Institute of Agricultural Sciences, Foshan 528145, China; Corresponding authors.Institute of Modern Aquaculture Science and Engineering (IMASE), Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences, South China Normal University, Guangzhou 510635, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Collaborative Innovation Center of Aquatic Sciences, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaFoshan Institute of Agricultural Sciences, Foshan 528145, ChinaFoshan Institute of Agricultural Sciences, Foshan 528145, ChinaGuangdong Cawolo Hydrogen Technology Company Ltd, Foshan 528200, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Collaborative Innovation Center of Aquatic Sciences, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Corresponding authors.Hydrogen (H2) gas is recognized for its anti-oxidative, anti-apoptotic, and anti-inflammatory properties in multiple plant and animal models. Nevertheless, there is a lack of research on the effects of H2 administration on fish. This research seeks to investigate how H2 administration affects the growth performance, mammalian target of rapamycin (mTOR) and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways, and intestinal microbiota of juvenile largemouth bass under normal feeding conditions. The research randomly allocated 240 fish (3.55 ± 0.01 g) into two treatment groups, each comprising 4 replicate tanks with 30 fish per tank. The control group was maintained in regular water, while the experimental group (referred to as H2 group) was treated with H2-dissolved water for 1 h per day for 6 weeks. Upon analysis of both groups, it was revealed that the weight gain, final weight, feed intake, specific growth rate, and survival rate were significantly improved by H2 treatment (P < 0.05). In addition, administration of H2 significantly reduced the triglyceride content and increased the high-density lipoprotein-cholesterol content in the serum (P < 0.05). Moreover, administration of H2 led to a significant increase in the expression of genes associated with mTOR (PI3K, AKT, TOR, S6K1, and 4EBP1) and Nrf2 (Nrf2, SOD, and GPX) signaling pathways (P < 0.05). Furthermore, H2 significantly increased the expression of growth hormone receptor (GHR) and neuropeptide Y (NPY) genes in the brain of juvenile largemouth bass. H2 did not significantly affect intestinal histology. However, it significantly increased the relative abundance of Sulfuritalea, Bradyrhizobium, and norank_f__Beggiatoaceae (P < 0.05) and reduced the relative abundance of Brevundimonas, unclassified_o__Chlamydiales, and Delftia in the intestinal microbiota (P < 0.05). Thus, these results suggested that providing juvenile largemouth bass with H2-dissolved water for 1 h per day can lead to a significantly increasing in growth performance, stimulating feed intake, activating mTOR and Nrf2 signaling pathways, and augmenting the abundance of beneficial bacteria in the intestines of juvenile largemouth bass. These findings provided a valuable support for the H2 application in the largemouth bass healthy culture.http://www.sciencedirect.com/science/article/pii/S2352513423002880Hydrogen gasGrowth performancemTOR and Nrf2 pathwaysIntestinal microbiotaLargemouth bass (Micropterus salmoides) |
| spellingShingle | Junru Hu Long Huang Lei Wang Wen Huang Mingjian Lai Xiangni Li Yongchun Lin Yuping Sun Hydrogen administration improves the growth performance of juvenile largemouth bass (Micropterus salmoides) by increasing feed intake, reducing serum lipids, activating mTOR and Nrf2 signaling pathways, and altering the intestinal microbiota Aquaculture Reports Hydrogen gas Growth performance mTOR and Nrf2 pathways Intestinal microbiota Largemouth bass (Micropterus salmoides) |
| title | Hydrogen administration improves the growth performance of juvenile largemouth bass (Micropterus salmoides) by increasing feed intake, reducing serum lipids, activating mTOR and Nrf2 signaling pathways, and altering the intestinal microbiota |
| title_full | Hydrogen administration improves the growth performance of juvenile largemouth bass (Micropterus salmoides) by increasing feed intake, reducing serum lipids, activating mTOR and Nrf2 signaling pathways, and altering the intestinal microbiota |
| title_fullStr | Hydrogen administration improves the growth performance of juvenile largemouth bass (Micropterus salmoides) by increasing feed intake, reducing serum lipids, activating mTOR and Nrf2 signaling pathways, and altering the intestinal microbiota |
| title_full_unstemmed | Hydrogen administration improves the growth performance of juvenile largemouth bass (Micropterus salmoides) by increasing feed intake, reducing serum lipids, activating mTOR and Nrf2 signaling pathways, and altering the intestinal microbiota |
| title_short | Hydrogen administration improves the growth performance of juvenile largemouth bass (Micropterus salmoides) by increasing feed intake, reducing serum lipids, activating mTOR and Nrf2 signaling pathways, and altering the intestinal microbiota |
| title_sort | hydrogen administration improves the growth performance of juvenile largemouth bass micropterus salmoides by increasing feed intake reducing serum lipids activating mtor and nrf2 signaling pathways and altering the intestinal microbiota |
| topic | Hydrogen gas Growth performance mTOR and Nrf2 pathways Intestinal microbiota Largemouth bass (Micropterus salmoides) |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423002880 |
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