Comparative Study of the Extracellular Holocellulolytic Activity of <i>Fusarium solani</i> and <i>Aspergillus</i> sp. in Corn Stover
Fungal holocellulases are interesting for their possible applications in the bioconversion of corn crop residues into molecules with technological significance. Holocellulase (xylanases and cellulases) production from <i>Fusarium solani</i> and <i>Aspergillus</i> sp. with cor...
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MDPI AG
2024-01-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/2/84 |
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| author | Mariana Alvarez-Navarrete Katia L. Alonso-Hurtado Alberto Flores-García Josué Altamirano-Hernández Mauro M. Martínez-Pacheco Crisanto Velázquez-Becerra |
| author_facet | Mariana Alvarez-Navarrete Katia L. Alonso-Hurtado Alberto Flores-García Josué Altamirano-Hernández Mauro M. Martínez-Pacheco Crisanto Velázquez-Becerra |
| author_sort | Mariana Alvarez-Navarrete |
| collection | DOAJ |
| description | Fungal holocellulases are interesting for their possible applications in the bioconversion of corn crop residues into molecules with technological significance. Holocellulase (xylanases and cellulases) production from <i>Fusarium solani</i> and <i>Aspergillus</i> sp. with corn stover as a carbon source was compared using a Box–Wilson design. The fungal holocellulase production was different in both fungi. For <i>F. solani</i>, the maximum endoxylanase and <i>β</i>-xylosidase activities were 14.15 U/mg and 0.75 U/mg at 84 h of fermentation on 350 g/L corn stover, while <i>Aspergillus</i> sp. was 5.90 U/mg and 0.03 U/mg, respectively, at 156 h and 1000 g/L corn stover. The production of holocellulases in both fungi was reduced with increasing carbon sources. The nitrogen source induced the holocellulases in <i>Aspergillus</i> sp., but not in <i>F. solani</i>. Interestingly, when verifying the optimal culture conditions, the production of endoxylanases by <i>F. solani</i> was higher when compared to the predicted value. With regard to the endoxylanase and <i>β</i>-xylosidase activities of <i>Aspergillus</i> sp., these were close to the predicted values. Based on the optimization model, <i>F. solani</i> and <i>Aspergillus</i> sp. produce an interesting holocellulolytic activity in a growth medium with corn stover as the only carbon source. The fermentation time and the amount of corn stover required to obtain maximum holocellulase production are possible advantages for <i>Fusarium solani</i> and <i>Aspergillus</i> sp., respectively. |
| first_indexed | 2024-03-07T22:33:16Z |
| format | Article |
| id | doaj.art-1562916cd7ab4e99906d050a8bea64bb |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-07T22:33:16Z |
| publishDate | 2024-01-01 |
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| series | Fermentation |
| spelling | doaj.art-1562916cd7ab4e99906d050a8bea64bb2024-02-23T15:15:53ZengMDPI AGFermentation2311-56372024-01-011028410.3390/fermentation10020084Comparative Study of the Extracellular Holocellulolytic Activity of <i>Fusarium solani</i> and <i>Aspergillus</i> sp. in Corn StoverMariana Alvarez-Navarrete0Katia L. Alonso-Hurtado1Alberto Flores-García2Josué Altamirano-Hernández3Mauro M. Martínez-Pacheco4Crisanto Velázquez-Becerra5Tecnológico Nacional de México, Instituto Tecnológico de Morelia, Morelia 58120, Michoacán, MexicoInstituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58040, Michoacán, MexicoInstituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58040, Michoacán, MexicoInstituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58040, Michoacán, MexicoInstituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58040, Michoacán, MexicoFacultad de Ingeniería en Tecnología de la Madera, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58120, Michoacán, MexicoFungal holocellulases are interesting for their possible applications in the bioconversion of corn crop residues into molecules with technological significance. Holocellulase (xylanases and cellulases) production from <i>Fusarium solani</i> and <i>Aspergillus</i> sp. with corn stover as a carbon source was compared using a Box–Wilson design. The fungal holocellulase production was different in both fungi. For <i>F. solani</i>, the maximum endoxylanase and <i>β</i>-xylosidase activities were 14.15 U/mg and 0.75 U/mg at 84 h of fermentation on 350 g/L corn stover, while <i>Aspergillus</i> sp. was 5.90 U/mg and 0.03 U/mg, respectively, at 156 h and 1000 g/L corn stover. The production of holocellulases in both fungi was reduced with increasing carbon sources. The nitrogen source induced the holocellulases in <i>Aspergillus</i> sp., but not in <i>F. solani</i>. Interestingly, when verifying the optimal culture conditions, the production of endoxylanases by <i>F. solani</i> was higher when compared to the predicted value. With regard to the endoxylanase and <i>β</i>-xylosidase activities of <i>Aspergillus</i> sp., these were close to the predicted values. Based on the optimization model, <i>F. solani</i> and <i>Aspergillus</i> sp. produce an interesting holocellulolytic activity in a growth medium with corn stover as the only carbon source. The fermentation time and the amount of corn stover required to obtain maximum holocellulase production are possible advantages for <i>Fusarium solani</i> and <i>Aspergillus</i> sp., respectively.https://www.mdpi.com/2311-5637/10/2/84endoxylanase<i>β</i>-xylosidaseendocellulaseholocellulases<i>Fusarium solani</i><i>Aspergillus</i> sp. |
| spellingShingle | Mariana Alvarez-Navarrete Katia L. Alonso-Hurtado Alberto Flores-García Josué Altamirano-Hernández Mauro M. Martínez-Pacheco Crisanto Velázquez-Becerra Comparative Study of the Extracellular Holocellulolytic Activity of <i>Fusarium solani</i> and <i>Aspergillus</i> sp. in Corn Stover Fermentation endoxylanase <i>β</i>-xylosidase endocellulase holocellulases <i>Fusarium solani</i> <i>Aspergillus</i> sp. |
| title | Comparative Study of the Extracellular Holocellulolytic Activity of <i>Fusarium solani</i> and <i>Aspergillus</i> sp. in Corn Stover |
| title_full | Comparative Study of the Extracellular Holocellulolytic Activity of <i>Fusarium solani</i> and <i>Aspergillus</i> sp. in Corn Stover |
| title_fullStr | Comparative Study of the Extracellular Holocellulolytic Activity of <i>Fusarium solani</i> and <i>Aspergillus</i> sp. in Corn Stover |
| title_full_unstemmed | Comparative Study of the Extracellular Holocellulolytic Activity of <i>Fusarium solani</i> and <i>Aspergillus</i> sp. in Corn Stover |
| title_short | Comparative Study of the Extracellular Holocellulolytic Activity of <i>Fusarium solani</i> and <i>Aspergillus</i> sp. in Corn Stover |
| title_sort | comparative study of the extracellular holocellulolytic activity of i fusarium solani i and i aspergillus i sp in corn stover |
| topic | endoxylanase <i>β</i>-xylosidase endocellulase holocellulases <i>Fusarium solani</i> <i>Aspergillus</i> sp. |
| url | https://www.mdpi.com/2311-5637/10/2/84 |
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