Pre- and postcapillary pulmonary hypertension in dogs: Circulating biomarkers
Background: Pulmonary hypertension (PH) in dogs is a syndrome that could be primary or secondary due to pulmonary disease, pulmonary thromboembolism, heartworm disease, heart failure. Due to the inability of right heart catheterization in veterinary patients, there is a lack of differential criteria...
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| Format: | Article |
| Language: | English |
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Tripoli University
2022-07-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | https://www.openveterinaryjournal.com/OVJ-2022-02-029%20D.A.%20Oleynikov%20and%20M.%20Yi.pdf |
| _version_ | 1811312813859143680 |
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| author | Dmitrij Arkadievich Oleynikov Ma Yi |
| author_facet | Dmitrij Arkadievich Oleynikov Ma Yi |
| author_sort | Dmitrij Arkadievich Oleynikov |
| collection | DOAJ |
| description | Background: Pulmonary hypertension (PH) in dogs is a syndrome that could be primary or secondary due to pulmonary disease, pulmonary thromboembolism, heartworm disease, heart failure. Due to the inability of right heart catheterization in veterinary patients, there is a lack of differential criteria between PH forms. In some acute cases, it is impossible to provide a full EchoCG or catheterization study. In this situation, circulating markers may be useful to discover the possible mechanism of pulmonary hypertension form and provide specific therapy.
Aim: Following all previous data in human and veterinary studies, we assumed that plasm concentration of serotonin, endothelin-1 and VEGF-D would show a predominance of affected part of pulmonary circulation.
Methods: We studied 59 small-breed dogs of different sexes and ages. Groups were formed according to a primary pathology: healthy dogs (HD, n=8); dogs with MMVD and postcapillary PH (PostPH, n=23); dogs with MMVD and precapillary PH (PrePH, n=28). Animals in the study were diagnosed with the primary disease by standard echocardiographic methods and algorithms. Blood samples were collected at the moment of presentation and frozen in a -80oC fridge. For biochemistry analysis we used species-specific ELISA kits, provided by Cloud-Clone Corp. (USA) The tests were provided by the means of Almazov National Medical Research Center, IEM laboratory.
Results: Dogs with EchoCG-proved PostPH had a higher concentration of VEGF-D in comparison to control and PrePH (р <0.001, for both). There was no difference between the control and PrePH groups (р >0.05). Endothelin-1 was higher in PrePH in comparison to PostPH and control dogs (р <0.001, for both). In addition, there was no difference between control and PostPH groups (р >0.05). Serotonin concertation did not have a difference between controls and PostPH. However, it was higher in PrePH than in control (р <0.033) and postcapillary PH group (р <0.006). ROC analysis showed that plasma concentrations of Endothelin-1 (0.99) and VEGF-D (0.92) had high effectiveness in the differentiation of PostPH and PrePH.
Conclusion: This study showed a correlation between circulating biomarkers (serotonin, endothelin-1 and VEGF-D). We found a connection between endothelin-1 and right-sided heart failure as well as VEGF-D and left heart failure in the PH context. |
| first_indexed | 2024-04-13T10:43:14Z |
| format | Article |
| id | doaj.art-1565289192c243bcac5735d567328a6b |
| institution | Directory Open Access Journal |
| issn | 2226-4485 2218-6050 |
| language | English |
| last_indexed | 2024-04-13T10:43:14Z |
| publishDate | 2022-07-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-1565289192c243bcac5735d567328a6b2022-12-22T02:49:52ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502022-07-0112446948010.5455/OVJ.2022.v12.i4.8Pre- and postcapillary pulmonary hypertension in dogs: Circulating biomarkersDmitrij Arkadievich Oleynikov0https://orcid.org/0000-0002-5667-3744Ma Yi1Veterinary clinic “Belij Klyk”, Moscow, Russian Federation, RussiaLaboratory of Transfusion and Efferent Therapy, Nacional’nyj medicinskij issledovatel’skij centr imeni V A Almazova:Sankt Peterburg, RU, Saint Petersburg, RussiaBackground: Pulmonary hypertension (PH) in dogs is a syndrome that could be primary or secondary due to pulmonary disease, pulmonary thromboembolism, heartworm disease, heart failure. Due to the inability of right heart catheterization in veterinary patients, there is a lack of differential criteria between PH forms. In some acute cases, it is impossible to provide a full EchoCG or catheterization study. In this situation, circulating markers may be useful to discover the possible mechanism of pulmonary hypertension form and provide specific therapy. Aim: Following all previous data in human and veterinary studies, we assumed that plasm concentration of serotonin, endothelin-1 and VEGF-D would show a predominance of affected part of pulmonary circulation. Methods: We studied 59 small-breed dogs of different sexes and ages. Groups were formed according to a primary pathology: healthy dogs (HD, n=8); dogs with MMVD and postcapillary PH (PostPH, n=23); dogs with MMVD and precapillary PH (PrePH, n=28). Animals in the study were diagnosed with the primary disease by standard echocardiographic methods and algorithms. Blood samples were collected at the moment of presentation and frozen in a -80oC fridge. For biochemistry analysis we used species-specific ELISA kits, provided by Cloud-Clone Corp. (USA) The tests were provided by the means of Almazov National Medical Research Center, IEM laboratory. Results: Dogs with EchoCG-proved PostPH had a higher concentration of VEGF-D in comparison to control and PrePH (р <0.001, for both). There was no difference between the control and PrePH groups (р >0.05). Endothelin-1 was higher in PrePH in comparison to PostPH and control dogs (р <0.001, for both). In addition, there was no difference between control and PostPH groups (р >0.05). Serotonin concertation did not have a difference between controls and PostPH. However, it was higher in PrePH than in control (р <0.033) and postcapillary PH group (р <0.006). ROC analysis showed that plasma concentrations of Endothelin-1 (0.99) and VEGF-D (0.92) had high effectiveness in the differentiation of PostPH and PrePH. Conclusion: This study showed a correlation between circulating biomarkers (serotonin, endothelin-1 and VEGF-D). We found a connection between endothelin-1 and right-sided heart failure as well as VEGF-D and left heart failure in the PH context.https://www.openveterinaryjournal.com/OVJ-2022-02-029%20D.A.%20Oleynikov%20and%20M.%20Yi.pdfbiomarkersendothelin-1pulmonary hypertensionserotoninvegf-d |
| spellingShingle | Dmitrij Arkadievich Oleynikov Ma Yi Pre- and postcapillary pulmonary hypertension in dogs: Circulating biomarkers Open Veterinary Journal biomarkers endothelin-1 pulmonary hypertension serotonin vegf-d |
| title | Pre- and postcapillary pulmonary hypertension in dogs: Circulating biomarkers |
| title_full | Pre- and postcapillary pulmonary hypertension in dogs: Circulating biomarkers |
| title_fullStr | Pre- and postcapillary pulmonary hypertension in dogs: Circulating biomarkers |
| title_full_unstemmed | Pre- and postcapillary pulmonary hypertension in dogs: Circulating biomarkers |
| title_short | Pre- and postcapillary pulmonary hypertension in dogs: Circulating biomarkers |
| title_sort | pre and postcapillary pulmonary hypertension in dogs circulating biomarkers |
| topic | biomarkers endothelin-1 pulmonary hypertension serotonin vegf-d |
| url | https://www.openveterinaryjournal.com/OVJ-2022-02-029%20D.A.%20Oleynikov%20and%20M.%20Yi.pdf |
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