Pea hull fiber supplementation does not modulate uremic metabolites in adults receiving hemodialysis: a randomized, double-blind, controlled trial
BackgroundFiber is a potential therapeutic to suppress microbiota-generated uremic molecules. This study aimed to determine if fiber supplementation decreased serum levels of uremic molecules through the modulation of gut microbiota in adults undergoing hemodialysis.MethodsA randomized, double-blind...
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1179295/full |
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| author | Asmaa M. N. Fatani Asmaa M. N. Fatani Joon Hyuk Suh Jérémie Auger Karima M. Alabasi Karima M. Alabasi Yu Wang Mark S. Segal Mark S. Segal Wendy J. Dahl |
| author_facet | Asmaa M. N. Fatani Asmaa M. N. Fatani Joon Hyuk Suh Jérémie Auger Karima M. Alabasi Karima M. Alabasi Yu Wang Mark S. Segal Mark S. Segal Wendy J. Dahl |
| author_sort | Asmaa M. N. Fatani |
| collection | DOAJ |
| description | BackgroundFiber is a potential therapeutic to suppress microbiota-generated uremic molecules. This study aimed to determine if fiber supplementation decreased serum levels of uremic molecules through the modulation of gut microbiota in adults undergoing hemodialysis.MethodsA randomized, double-blinded, controlled crossover study was conducted. Following a 1-week baseline, participants consumed muffins with added pea hull fiber (PHF) (15 g/d) and control muffins daily, each for 4 weeks, separated by a 4-week washout. Blood and stool samples were collected per period. Serum p-cresyl sulfate (PCS), indoxyl sulfate (IS), phenylacetylglutamine (PAG), and trimethylamine N-oxide (TMAO) were quantified by LC–MS/MS, and fecal microbiota profiled by 16S rRNA gene amplicon sequencing and specific taxa of interest by qPCR. QIIME 2 sample-classifier was used to discover unique microbiota profiles due to the consumption of PHF.ResultsIntake of PHF contributed an additional 9 g/d of dietary fiber to the subjects’ diet due to compliance. No significant changes from baseline were observed in serum PCS, IS, PAG, or TMAO, or for the relative quantification of Akkermansia muciniphila, Faecalibacterium prausnitzii, Bifidobacterium, or Roseburia, taxa considered health-enhancing. Dietary protein intake and IS (r = −0.5, p = 0.05) and slow transit stool form and PCS (r = 0.7, p < 0.01) were significantly correlated at baseline. PHF and control periods were not differentiated; however, using machine learning, taxa most distinguishing the microbiota composition during the PHF periods compared to usual diet alone were enriched Gemmiger, Collinsella, and depleted Lactobacillus, Ruminococcus, Coprococcus, and Mogibacteriaceae.ConclusionPHF supplementation did not mitigate serum levels of targeted microbial-generated uremic molecules. Given the high cellulose content, which may be resistant to fermentation, PHF may not exert sufficient effects on microbiota composition to modulate its activity at the dose consumed. |
| first_indexed | 2024-03-13T02:11:42Z |
| format | Article |
| id | doaj.art-1567d75b272f4806841dd27cc151671d |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-03-13T02:11:42Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-1567d75b272f4806841dd27cc151671d2023-06-30T22:06:38ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-06-011010.3389/fnut.2023.11792951179295Pea hull fiber supplementation does not modulate uremic metabolites in adults receiving hemodialysis: a randomized, double-blind, controlled trialAsmaa M. N. Fatani0Asmaa M. N. Fatani1Joon Hyuk Suh2Jérémie Auger3Karima M. Alabasi4Karima M. Alabasi5Yu Wang6Mark S. Segal7Mark S. Segal8Wendy J. Dahl9Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL, United StatesFood and Nutrition Department, King Abdulaziz University, Jeddah, Saudi ArabiaDepartment of Food Science and Human Nutrition, University of Florida, Gainesville, FL, United StatesRosell Institute for Microbiome and Probiotics, Lallemand Health Solutions, Montréal, QC, CanadaDepartment of Food Science and Human Nutrition, University of Florida, Gainesville, FL, United StatesFoods and Nutrition Department, School of Health Science and Wellness, Northwest Missouri State University, Maryville, MO, United StatesDepartment of Food Science and Human Nutrition, University of Florida, Gainesville, FL, United StatesDepartment of Nephrology, Hypertension and Renal Transplantation, College of Medicine, University of Florida, Gainesville, FL, United StatesNorth Florida South Georgia VHS, Gainesville, FL, United StatesDepartment of Food Science and Human Nutrition, University of Florida, Gainesville, FL, United StatesBackgroundFiber is a potential therapeutic to suppress microbiota-generated uremic molecules. This study aimed to determine if fiber supplementation decreased serum levels of uremic molecules through the modulation of gut microbiota in adults undergoing hemodialysis.MethodsA randomized, double-blinded, controlled crossover study was conducted. Following a 1-week baseline, participants consumed muffins with added pea hull fiber (PHF) (15 g/d) and control muffins daily, each for 4 weeks, separated by a 4-week washout. Blood and stool samples were collected per period. Serum p-cresyl sulfate (PCS), indoxyl sulfate (IS), phenylacetylglutamine (PAG), and trimethylamine N-oxide (TMAO) were quantified by LC–MS/MS, and fecal microbiota profiled by 16S rRNA gene amplicon sequencing and specific taxa of interest by qPCR. QIIME 2 sample-classifier was used to discover unique microbiota profiles due to the consumption of PHF.ResultsIntake of PHF contributed an additional 9 g/d of dietary fiber to the subjects’ diet due to compliance. No significant changes from baseline were observed in serum PCS, IS, PAG, or TMAO, or for the relative quantification of Akkermansia muciniphila, Faecalibacterium prausnitzii, Bifidobacterium, or Roseburia, taxa considered health-enhancing. Dietary protein intake and IS (r = −0.5, p = 0.05) and slow transit stool form and PCS (r = 0.7, p < 0.01) were significantly correlated at baseline. PHF and control periods were not differentiated; however, using machine learning, taxa most distinguishing the microbiota composition during the PHF periods compared to usual diet alone were enriched Gemmiger, Collinsella, and depleted Lactobacillus, Ruminococcus, Coprococcus, and Mogibacteriaceae.ConclusionPHF supplementation did not mitigate serum levels of targeted microbial-generated uremic molecules. Given the high cellulose content, which may be resistant to fermentation, PHF may not exert sufficient effects on microbiota composition to modulate its activity at the dose consumed.https://www.frontiersin.org/articles/10.3389/fnut.2023.1179295/fullmicrobiotahemodialysisuremiafiberp-cresyl sulfateindoxyl sulfate |
| spellingShingle | Asmaa M. N. Fatani Asmaa M. N. Fatani Joon Hyuk Suh Jérémie Auger Karima M. Alabasi Karima M. Alabasi Yu Wang Mark S. Segal Mark S. Segal Wendy J. Dahl Pea hull fiber supplementation does not modulate uremic metabolites in adults receiving hemodialysis: a randomized, double-blind, controlled trial Frontiers in Nutrition microbiota hemodialysis uremia fiber p-cresyl sulfate indoxyl sulfate |
| title | Pea hull fiber supplementation does not modulate uremic metabolites in adults receiving hemodialysis: a randomized, double-blind, controlled trial |
| title_full | Pea hull fiber supplementation does not modulate uremic metabolites in adults receiving hemodialysis: a randomized, double-blind, controlled trial |
| title_fullStr | Pea hull fiber supplementation does not modulate uremic metabolites in adults receiving hemodialysis: a randomized, double-blind, controlled trial |
| title_full_unstemmed | Pea hull fiber supplementation does not modulate uremic metabolites in adults receiving hemodialysis: a randomized, double-blind, controlled trial |
| title_short | Pea hull fiber supplementation does not modulate uremic metabolites in adults receiving hemodialysis: a randomized, double-blind, controlled trial |
| title_sort | pea hull fiber supplementation does not modulate uremic metabolites in adults receiving hemodialysis a randomized double blind controlled trial |
| topic | microbiota hemodialysis uremia fiber p-cresyl sulfate indoxyl sulfate |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1179295/full |
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