Effect of Enteromorpha Polysaccharide on Intestinal Bacteria and Short Chain Fatty Acids in Obese Golden Hamsters
Objective: To investigate the effect of Enteromorpha polysaccharide (EP) on blood lipids and intestinal bacteria in obese golden hamsters. Method: Forty male golden hamsters were randomly divided into four groups, including control group (ND), model group (HFD), low-dose EP group (LEP, 300 mg/kg·BW)...
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The editorial department of Science and Technology of Food Industry
2023-02-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030170 |
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| author | Shiying HUANG Jiedong CHEN Mengyuan HAN Caihong XU Fuchuan GUO |
| author_facet | Shiying HUANG Jiedong CHEN Mengyuan HAN Caihong XU Fuchuan GUO |
| author_sort | Shiying HUANG |
| collection | DOAJ |
| description | Objective: To investigate the effect of Enteromorpha polysaccharide (EP) on blood lipids and intestinal bacteria in obese golden hamsters. Method: Forty male golden hamsters were randomly divided into four groups, including control group (ND), model group (HFD), low-dose EP group (LEP, 300 mg/kg·BW), and high-dose EP group (HEP, 450 mg/kg·BW). The ND group was fed an ordinary diet, while the other three groups were given a high-fat diet. Among the high-fat diet groups, the LEP and HEP groups were continuously administered EP aqueous solution intragastrically. Twelve weeks later, the serum lipid levels were evaluated, the diversity and structural changes in the gut bacteria were examined using 16S rDNA sequencing, and the short-chain fatty acid concentration in faeces was examined using a gas chromatography flame ionisation detector (GC-FID). Results: After 12 weeks, the body weight, serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) of hamsters in the HFD group were considerably higher than those in the ND group (P<0.05). In contrast, high-dose EP treatment led to a significant decrease in serum TC, TG, LDL-C, and alanine aminotransferase (ALT) levels (P<0.05). Results of 16S rDNA sequencing revealed that at the phylum level the proportion of Firmicutes/Bacteroidetes was substantially higher in the HFD group than in the ND group (P<0.05). However, the level of Firmicutes/Bacteroidetes was much lower in the HEP group, compared to the HFD group (P<0.05). At the genus level, Eubacterium_coprostanoligenes_group and Lachnospiraceae UCG-006 were more abundant in the HFD group than in the ND group (P<0.05). Following high-dose EP intervention, the relative abundance of Eubacterium_coprostanoligenes_group and Lachnospiraceae_UCG-006 fell significantly, compared to the HFD group (P<0.05). Additionally, a high-fat diet feeding resulted in a decrease in the content of short-chain fatty acids in faeces, while high-dose EP intervention significantly increased the short-chain fatty acid content in faeces (P<0.05). Conclusion: The administration of EP alleviates the metabolic disorders of obese golden hamsters fed with a high-fat diet by moderating the composition of intestinal bacteria and enhancing the production of short-chain fatty acids. |
| first_indexed | 2024-04-10T22:28:09Z |
| format | Article |
| id | doaj.art-1568174859484a09b12eac4305f1b161 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-10T22:28:09Z |
| publishDate | 2023-02-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-1568174859484a09b12eac4305f1b1612023-01-17T08:07:44ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-02-0144338139010.13386/j.issn1002-0306.20220301702022030170-3Effect of Enteromorpha Polysaccharide on Intestinal Bacteria and Short Chain Fatty Acids in Obese Golden HamstersShiying HUANG0Jiedong CHEN1Mengyuan HAN2Caihong XU3Fuchuan GUO4School of Public Health, Fujian Medical University, Fuzhou 350122, ChinaSchool of Public Health, Fujian Medical University, Fuzhou 350122, ChinaFujian Maternity and Child Health Hospital, Fuzhou 350001, ChinaSchool of Public Health, Fujian Medical University, Fuzhou 350122, ChinaSchool of Public Health, Fujian Medical University, Fuzhou 350122, ChinaObjective: To investigate the effect of Enteromorpha polysaccharide (EP) on blood lipids and intestinal bacteria in obese golden hamsters. Method: Forty male golden hamsters were randomly divided into four groups, including control group (ND), model group (HFD), low-dose EP group (LEP, 300 mg/kg·BW), and high-dose EP group (HEP, 450 mg/kg·BW). The ND group was fed an ordinary diet, while the other three groups were given a high-fat diet. Among the high-fat diet groups, the LEP and HEP groups were continuously administered EP aqueous solution intragastrically. Twelve weeks later, the serum lipid levels were evaluated, the diversity and structural changes in the gut bacteria were examined using 16S rDNA sequencing, and the short-chain fatty acid concentration in faeces was examined using a gas chromatography flame ionisation detector (GC-FID). Results: After 12 weeks, the body weight, serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) of hamsters in the HFD group were considerably higher than those in the ND group (P<0.05). In contrast, high-dose EP treatment led to a significant decrease in serum TC, TG, LDL-C, and alanine aminotransferase (ALT) levels (P<0.05). Results of 16S rDNA sequencing revealed that at the phylum level the proportion of Firmicutes/Bacteroidetes was substantially higher in the HFD group than in the ND group (P<0.05). However, the level of Firmicutes/Bacteroidetes was much lower in the HEP group, compared to the HFD group (P<0.05). At the genus level, Eubacterium_coprostanoligenes_group and Lachnospiraceae UCG-006 were more abundant in the HFD group than in the ND group (P<0.05). Following high-dose EP intervention, the relative abundance of Eubacterium_coprostanoligenes_group and Lachnospiraceae_UCG-006 fell significantly, compared to the HFD group (P<0.05). Additionally, a high-fat diet feeding resulted in a decrease in the content of short-chain fatty acids in faeces, while high-dose EP intervention significantly increased the short-chain fatty acid content in faeces (P<0.05). Conclusion: The administration of EP alleviates the metabolic disorders of obese golden hamsters fed with a high-fat diet by moderating the composition of intestinal bacteria and enhancing the production of short-chain fatty acids.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030170enteromorpha polysaccharideshigh-fat dietgut microbiotashort-chain fatty acids |
| spellingShingle | Shiying HUANG Jiedong CHEN Mengyuan HAN Caihong XU Fuchuan GUO Effect of Enteromorpha Polysaccharide on Intestinal Bacteria and Short Chain Fatty Acids in Obese Golden Hamsters Shipin gongye ke-ji enteromorpha polysaccharides high-fat diet gut microbiota short-chain fatty acids |
| title | Effect of Enteromorpha Polysaccharide on Intestinal Bacteria and Short Chain Fatty Acids in Obese Golden Hamsters |
| title_full | Effect of Enteromorpha Polysaccharide on Intestinal Bacteria and Short Chain Fatty Acids in Obese Golden Hamsters |
| title_fullStr | Effect of Enteromorpha Polysaccharide on Intestinal Bacteria and Short Chain Fatty Acids in Obese Golden Hamsters |
| title_full_unstemmed | Effect of Enteromorpha Polysaccharide on Intestinal Bacteria and Short Chain Fatty Acids in Obese Golden Hamsters |
| title_short | Effect of Enteromorpha Polysaccharide on Intestinal Bacteria and Short Chain Fatty Acids in Obese Golden Hamsters |
| title_sort | effect of enteromorpha polysaccharide on intestinal bacteria and short chain fatty acids in obese golden hamsters |
| topic | enteromorpha polysaccharides high-fat diet gut microbiota short-chain fatty acids |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022030170 |
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