Breeding of Butenyl-spinosyns Producing Strain by Atmospheric Room Temperature Plasma and Optimization of Culture Medium
The original strain ASAGF 2-G4 was applied to undergo Atmospheric Room Temperature Plasma (ARTP) mutagenesis of various doses and 2 mg/mL NTG compound mutagenesis of different action durations. Five high-yield mutant strains were obtained after preliminary screening through 96-orifice-plate fermenta...
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| Format: | Article |
| Language: | English |
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Academy of National Food and Strategic Reserves Administration
2022-01-01
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| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220128?st=article_issue |
| _version_ | 1797842820226088960 |
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| author | ZHANG Xiao-yao GUO Chao WANG Jing-nan LIU Yan-li WANG Chao |
| author_facet | ZHANG Xiao-yao GUO Chao WANG Jing-nan LIU Yan-li WANG Chao |
| author_sort | ZHANG Xiao-yao |
| collection | DOAJ |
| description | The original strain ASAGF 2-G4 was applied to undergo Atmospheric Room Temperature Plasma (ARTP) mutagenesis of various doses and 2 mg/mL NTG compound mutagenesis of different action durations. Five high-yield mutant strains were obtained after preliminary screening through 96-orifice-plate fermentation and re-screening in shake flasks. At last, a high-yielding mutant strain 2-A9 was selected, which increased the yield by 30.92% compared with the original strain after genetic stability verification. A high-yield mutant ASAGF 2-A9 was applied as the starting strain, the experiment design Plackett-Burman was used to screen the factors affecting the fermentation media, with a conclusion that factor glucose, dextrin, cottonseed protein and corn starch is a significant factor affecting butenyl-spinosyns. The experimental design Box-Behnken was applied to optimize the factors significantly affecting the experiment Plackett-Burman. The optimal growing media components were deduced as follows (g/L): glucose 60.0, dextrin 25.0, cottonseed protein 25.0, coen steep dry powder 12.5, peptone milk 10.0, MgSO4 1.0, NaCl 2.0, CaCO3 5.0. It’s verified that the average output of butenyl- spinosyns is close to the predicted value, indicating that the model is feasible and effective. |
| first_indexed | 2024-04-09T16:54:27Z |
| format | Article |
| id | doaj.art-156a582e9f37450b9177e6fc7eb82cec |
| institution | Directory Open Access Journal |
| issn | 1007-7561 |
| language | English |
| last_indexed | 2024-04-09T16:54:27Z |
| publishDate | 2022-01-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj.art-156a582e9f37450b9177e6fc7eb82cec2023-04-21T06:11:51ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612022-01-0130117418110.16210/j.cnki.1007-7561.2022.01.022Breeding of Butenyl-spinosyns Producing Strain by Atmospheric Room Temperature Plasma and Optimization of Culture MediumZHANG Xiao-yao0GUO Chao1WANG Jing-nan2LIU Yan-li3WANG Chao4cademy of National Food and Strategic Reserves Administration, Beijing 100037, China; MCMM Center, Beijing City University, Beijing 100083, Chinacademy of National Food and Strategic Reserves Administration, Beijing 100037, Chinacademy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaMCMM Center, Beijing City University, Beijing 100083, Chinacademy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaThe original strain ASAGF 2-G4 was applied to undergo Atmospheric Room Temperature Plasma (ARTP) mutagenesis of various doses and 2 mg/mL NTG compound mutagenesis of different action durations. Five high-yield mutant strains were obtained after preliminary screening through 96-orifice-plate fermentation and re-screening in shake flasks. At last, a high-yielding mutant strain 2-A9 was selected, which increased the yield by 30.92% compared with the original strain after genetic stability verification. A high-yield mutant ASAGF 2-A9 was applied as the starting strain, the experiment design Plackett-Burman was used to screen the factors affecting the fermentation media, with a conclusion that factor glucose, dextrin, cottonseed protein and corn starch is a significant factor affecting butenyl-spinosyns. The experimental design Box-Behnken was applied to optimize the factors significantly affecting the experiment Plackett-Burman. The optimal growing media components were deduced as follows (g/L): glucose 60.0, dextrin 25.0, cottonseed protein 25.0, coen steep dry powder 12.5, peptone milk 10.0, MgSO4 1.0, NaCl 2.0, CaCO3 5.0. It’s verified that the average output of butenyl- spinosyns is close to the predicted value, indicating that the model is feasible and effective.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220128?st=article_issuebutenyl-spinosynsartpntgmutagenesismedium optimization |
| spellingShingle | ZHANG Xiao-yao GUO Chao WANG Jing-nan LIU Yan-li WANG Chao Breeding of Butenyl-spinosyns Producing Strain by Atmospheric Room Temperature Plasma and Optimization of Culture Medium Liang you shipin ke-ji butenyl-spinosyns artp ntg mutagenesis medium optimization |
| title | Breeding of Butenyl-spinosyns Producing Strain by Atmospheric Room Temperature Plasma and Optimization of Culture Medium |
| title_full | Breeding of Butenyl-spinosyns Producing Strain by Atmospheric Room Temperature Plasma and Optimization of Culture Medium |
| title_fullStr | Breeding of Butenyl-spinosyns Producing Strain by Atmospheric Room Temperature Plasma and Optimization of Culture Medium |
| title_full_unstemmed | Breeding of Butenyl-spinosyns Producing Strain by Atmospheric Room Temperature Plasma and Optimization of Culture Medium |
| title_short | Breeding of Butenyl-spinosyns Producing Strain by Atmospheric Room Temperature Plasma and Optimization of Culture Medium |
| title_sort | breeding of butenyl spinosyns producing strain by atmospheric room temperature plasma and optimization of culture medium |
| topic | butenyl-spinosyns artp ntg mutagenesis medium optimization |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220128?st=article_issue |
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