Split-DNAzyme cooperating primer exchange reaction for sensitive miRNA detection
Abstract Sensitive quantification of microRNA (miRNA) plays a crucial role in early diagnosis and precise therapy of osteosarcoma. Herein, we build a label-free and sensitive miRNA quantification approach based on the activation of split-DNAzyme initiated primer exchange reaction (PER). Target miRNA...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-10-01
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| Series: | Journal of Analytical Science and Technology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40543-022-00343-4 |
| _version_ | 1811194952997142528 |
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| author | Xuguo Luo Hongze Wu Mingjie Xiong Liyun Jiang Zhihao Jiang Minyong Gong |
| author_facet | Xuguo Luo Hongze Wu Mingjie Xiong Liyun Jiang Zhihao Jiang Minyong Gong |
| author_sort | Xuguo Luo |
| collection | DOAJ |
| description | Abstract Sensitive quantification of microRNA (miRNA) plays a crucial role in early diagnosis and precise therapy of osteosarcoma. Herein, we build a label-free and sensitive miRNA quantification approach based on the activation of split-DNAzyme initiated primer exchange reaction (PER). Target miRNA cooperates the activation of split-DNAzyme with Mg2+ through assisting the assembly of DNAzyme to correct conformation, which enables the performance of PER-based nucleic acids amplification to produce a large amount of single-strand DNA (ssDNA) sequences. The G-quadruplexes (G4) in ssDNA sequences products bind with N-methyl mesoporphyrin IX (NMM) to form G4-NMM complex with the enhanced fluorescence respond. The results demonstrate that miRNA-21 can assist the activation of split-DNAzyme, and the active DNAzyme exhibits a high specificity and efficiency in inducing the subsequent PER. Based on the split-DNAzyme-assisted signal recycle and PER, the method eventually shows a high sensitivity and selectivity, providing a promising prospect for the for early stage tumor diagnosis and more precise tumor therapy. |
| first_indexed | 2024-04-12T00:34:53Z |
| format | Article |
| id | doaj.art-156bc87eae5943b08654eaaa89e47e5f |
| institution | Directory Open Access Journal |
| issn | 2093-3371 |
| language | English |
| last_indexed | 2024-04-12T00:34:53Z |
| publishDate | 2022-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Analytical Science and Technology |
| spelling | doaj.art-156bc87eae5943b08654eaaa89e47e5f2022-12-22T03:55:10ZengSpringerOpenJournal of Analytical Science and Technology2093-33712022-10-011311710.1186/s40543-022-00343-4Split-DNAzyme cooperating primer exchange reaction for sensitive miRNA detectionXuguo Luo0Hongze Wu1Mingjie Xiong2Liyun Jiang3Zhihao Jiang4Minyong Gong5The Third Orthopedics Department, Jiujiang Traditional Chinese Medicine HospitalJiujiang Traditional Chinese Medicine HospitalGeneral Surgery Department, Jiujiang Traditional Chinese Medicine HospitalThe Third Orthopedics Department, Jiujiang Traditional Chinese Medicine HospitalThe Sixth Orthopedics Department, Jiujiang Traditional Chinese Medicine HospitalJiujiang Traditional Chinese Medicine HospitalAbstract Sensitive quantification of microRNA (miRNA) plays a crucial role in early diagnosis and precise therapy of osteosarcoma. Herein, we build a label-free and sensitive miRNA quantification approach based on the activation of split-DNAzyme initiated primer exchange reaction (PER). Target miRNA cooperates the activation of split-DNAzyme with Mg2+ through assisting the assembly of DNAzyme to correct conformation, which enables the performance of PER-based nucleic acids amplification to produce a large amount of single-strand DNA (ssDNA) sequences. The G-quadruplexes (G4) in ssDNA sequences products bind with N-methyl mesoporphyrin IX (NMM) to form G4-NMM complex with the enhanced fluorescence respond. The results demonstrate that miRNA-21 can assist the activation of split-DNAzyme, and the active DNAzyme exhibits a high specificity and efficiency in inducing the subsequent PER. Based on the split-DNAzyme-assisted signal recycle and PER, the method eventually shows a high sensitivity and selectivity, providing a promising prospect for the for early stage tumor diagnosis and more precise tumor therapy.https://doi.org/10.1186/s40543-022-00343-4OsteosarcomaMicroRNA (miRNA)N-methyl mesoporphyrin IXPrimer exchange reaction (PER) |
| spellingShingle | Xuguo Luo Hongze Wu Mingjie Xiong Liyun Jiang Zhihao Jiang Minyong Gong Split-DNAzyme cooperating primer exchange reaction for sensitive miRNA detection Journal of Analytical Science and Technology Osteosarcoma MicroRNA (miRNA) N-methyl mesoporphyrin IX Primer exchange reaction (PER) |
| title | Split-DNAzyme cooperating primer exchange reaction for sensitive miRNA detection |
| title_full | Split-DNAzyme cooperating primer exchange reaction for sensitive miRNA detection |
| title_fullStr | Split-DNAzyme cooperating primer exchange reaction for sensitive miRNA detection |
| title_full_unstemmed | Split-DNAzyme cooperating primer exchange reaction for sensitive miRNA detection |
| title_short | Split-DNAzyme cooperating primer exchange reaction for sensitive miRNA detection |
| title_sort | split dnazyme cooperating primer exchange reaction for sensitive mirna detection |
| topic | Osteosarcoma MicroRNA (miRNA) N-methyl mesoporphyrin IX Primer exchange reaction (PER) |
| url | https://doi.org/10.1186/s40543-022-00343-4 |
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