Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method
The aim of this paper was to determine the effect of the hydrolysis method on the amounts of phenolic compounds in the plant material in soil and, as a consequence, on the parameters to determine the degree of lignins transformation in soils. The study included the plant material (hay, sward, and ro...
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MDPI AG
2020-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/22/5462 |
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| author | Anna Ziolkowska Bozena Debska Magdalena Banach-Szott |
| author_facet | Anna Ziolkowska Bozena Debska Magdalena Banach-Szott |
| author_sort | Anna Ziolkowska |
| collection | DOAJ |
| description | The aim of this paper was to determine the effect of the hydrolysis method on the amounts of phenolic compounds in the plant material in soil and, as a consequence, on the parameters to determine the degree of lignins transformation in soils. The study included the plant material (hay, sward, and roots) and soil—Albic Brunic Arenosol (horizon A, AE, and Bsv) samples. Phenolic compounds were isolated at two stages by applying acid hydrolysis followed by alkaline re-hydrolysis. The quantitative and qualitative analysis of phenolic compounds was performed with high-performance liquid chromatography with a DAD. The content of phenolic compounds in the extracts depended on the hydrolysis method and it was determined by the type of the research material. The amounts of phenolic compounds contained in the alkaline hydrolysates accounted for 55.7% (soil, horizon Bsv)—454% (roots) of their content in acid hydrolysates. In the extracts from acid hydrolysates, chlorogenic and <i>p</i>-hydroxybenzoic acids were dominant. In the alkaline extracts from the plant material, the highest content was recorded for <i>p</i>-coumaric and ferulic acids, and in the extracts from soil, ferulic and chlorogenic acids. A combination of acid and alkaline hydrolysis ensures the best extraction efficiency of insoluble-bound forms of polyphenols from plant and soil material. |
| first_indexed | 2024-03-10T14:39:37Z |
| format | Article |
| id | doaj.art-15704dad744541618ea42355539fbb29 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:39:37Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-15704dad744541618ea42355539fbb292023-11-20T21:51:46ZengMDPI AGMolecules1420-30492020-11-012522546210.3390/molecules25225462Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation MethodAnna Ziolkowska0Bozena Debska1Magdalena Banach-Szott2Department of Biogeochemistry and Soil Science, University of Science and Technology, 6 Bernardynska St., 85-029 Bydgoszcz, PolandDepartment of Biogeochemistry and Soil Science, University of Science and Technology, 6 Bernardynska St., 85-029 Bydgoszcz, PolandDepartment of Biogeochemistry and Soil Science, University of Science and Technology, 6 Bernardynska St., 85-029 Bydgoszcz, PolandThe aim of this paper was to determine the effect of the hydrolysis method on the amounts of phenolic compounds in the plant material in soil and, as a consequence, on the parameters to determine the degree of lignins transformation in soils. The study included the plant material (hay, sward, and roots) and soil—Albic Brunic Arenosol (horizon A, AE, and Bsv) samples. Phenolic compounds were isolated at two stages by applying acid hydrolysis followed by alkaline re-hydrolysis. The quantitative and qualitative analysis of phenolic compounds was performed with high-performance liquid chromatography with a DAD. The content of phenolic compounds in the extracts depended on the hydrolysis method and it was determined by the type of the research material. The amounts of phenolic compounds contained in the alkaline hydrolysates accounted for 55.7% (soil, horizon Bsv)—454% (roots) of their content in acid hydrolysates. In the extracts from acid hydrolysates, chlorogenic and <i>p</i>-hydroxybenzoic acids were dominant. In the alkaline extracts from the plant material, the highest content was recorded for <i>p</i>-coumaric and ferulic acids, and in the extracts from soil, ferulic and chlorogenic acids. A combination of acid and alkaline hydrolysis ensures the best extraction efficiency of insoluble-bound forms of polyphenols from plant and soil material.https://www.mdpi.com/1420-3049/25/22/5462meadow soilsacid hydrolysisalkaline hydrolysisphenolic compoundsHPLC |
| spellingShingle | Anna Ziolkowska Bozena Debska Magdalena Banach-Szott Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method Molecules meadow soils acid hydrolysis alkaline hydrolysis phenolic compounds HPLC |
| title | Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method |
| title_full | Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method |
| title_fullStr | Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method |
| title_full_unstemmed | Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method |
| title_short | Content of Phenolic Compounds in Meadow Vegetation and Soil Depending on the Isolation Method |
| title_sort | content of phenolic compounds in meadow vegetation and soil depending on the isolation method |
| topic | meadow soils acid hydrolysis alkaline hydrolysis phenolic compounds HPLC |
| url | https://www.mdpi.com/1420-3049/25/22/5462 |
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