Plant Hormone Signals Mediate Melatonin Synthesis to Enhance Osmotic Stress Tolerance in Watermelon Cells
Melatonin, a multifunctional signaling molecule, has been shown to play a significant role in response to abiotic stress. Several species have been reported to unveil melatonin’s effect on osmotic stress; however, the signal transduction mechanism of phytohormone-mediated melatonin biosynthesis in p...
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MDPI AG
2023-08-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/8/927 |
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| author | Manwen Yan Mingyan Li Zhuoying Ding Fei Qiao Xuefei Jiang |
| author_facet | Manwen Yan Mingyan Li Zhuoying Ding Fei Qiao Xuefei Jiang |
| author_sort | Manwen Yan |
| collection | DOAJ |
| description | Melatonin, a multifunctional signaling molecule, has been shown to play a significant role in response to abiotic stress. Several species have been reported to unveil melatonin’s effect on osmotic stress; however, the signal transduction mechanism of phytohormone-mediated melatonin biosynthesis in plant species remains unclear. In this study, although plants can biosynthesize melatonin, the exogenous application of melatonin to watermelon cells can improve cell growth in response to osmotic stress by regulating the antioxidant machinery of cells. Regarding the melatonin synthesis pathway, ClOMT (ClASMT and ClCOMT) is a multi-gene family, and ClSNAT has two members. Both <i>ClOMTs</i> and <i>ClSNATs</i> harbor the <i>cis</i>-elements in their promoter regions responding to various hormones. Among abscisic acid (ABA), methyl jasmonate (MeJA), and salicylic acid (SA), ABA treatment observably upregulated the expression of <i>ClOMTs</i> and <i>ClSNATs</i>, and the accumulation of melatonin with ABA treatment reached a level comparable to that with osmotic stress by mannitol treatment. Furthermore, when hormone biosynthesis inhibitors were added to cells before osmotic stress, the expression of <i>ClOMTs</i> and <i>ClSNATs</i>, as well as melatonin accumulation, were significantly suppressed with the ABA biosynthesis inhibitor. This study demonstrated the crucial role of melatonin biosynthesis in response to osmotic stress via plant hormone signal transduction. It showed that ABA signaling plays a dominant role in melatonin synthesis under osmotic stress. |
| first_indexed | 2024-03-10T23:53:04Z |
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| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T23:53:04Z |
| publishDate | 2023-08-01 |
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| series | Horticulturae |
| spelling | doaj.art-15714dfa5bda472bb508a4758c9c93e62023-11-19T01:21:53ZengMDPI AGHorticulturae2311-75242023-08-019892710.3390/horticulturae9080927Plant Hormone Signals Mediate Melatonin Synthesis to Enhance Osmotic Stress Tolerance in Watermelon CellsManwen Yan0Mingyan Li1Zhuoying Ding2Fei Qiao3Xuefei Jiang4Sanya Nanfan Research Institute, Hainan University, Sanya 572000, ChinaKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province/Key Laboratory of Tropical Agritourism in Greenhouse of Haikou, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSanya Nanfan Research Institute, Hainan University, Sanya 572000, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, ChinaSanya Nanfan Research Institute, Hainan University, Sanya 572000, ChinaMelatonin, a multifunctional signaling molecule, has been shown to play a significant role in response to abiotic stress. Several species have been reported to unveil melatonin’s effect on osmotic stress; however, the signal transduction mechanism of phytohormone-mediated melatonin biosynthesis in plant species remains unclear. In this study, although plants can biosynthesize melatonin, the exogenous application of melatonin to watermelon cells can improve cell growth in response to osmotic stress by regulating the antioxidant machinery of cells. Regarding the melatonin synthesis pathway, ClOMT (ClASMT and ClCOMT) is a multi-gene family, and ClSNAT has two members. Both <i>ClOMTs</i> and <i>ClSNATs</i> harbor the <i>cis</i>-elements in their promoter regions responding to various hormones. Among abscisic acid (ABA), methyl jasmonate (MeJA), and salicylic acid (SA), ABA treatment observably upregulated the expression of <i>ClOMTs</i> and <i>ClSNATs</i>, and the accumulation of melatonin with ABA treatment reached a level comparable to that with osmotic stress by mannitol treatment. Furthermore, when hormone biosynthesis inhibitors were added to cells before osmotic stress, the expression of <i>ClOMTs</i> and <i>ClSNATs</i>, as well as melatonin accumulation, were significantly suppressed with the ABA biosynthesis inhibitor. This study demonstrated the crucial role of melatonin biosynthesis in response to osmotic stress via plant hormone signal transduction. It showed that ABA signaling plays a dominant role in melatonin synthesis under osmotic stress.https://www.mdpi.com/2311-7524/9/8/927<i>Citrullus lanatus</i>osmotic stressmelatoninplant hormone signalsgene expression |
| spellingShingle | Manwen Yan Mingyan Li Zhuoying Ding Fei Qiao Xuefei Jiang Plant Hormone Signals Mediate Melatonin Synthesis to Enhance Osmotic Stress Tolerance in Watermelon Cells Horticulturae <i>Citrullus lanatus</i> osmotic stress melatonin plant hormone signals gene expression |
| title | Plant Hormone Signals Mediate Melatonin Synthesis to Enhance Osmotic Stress Tolerance in Watermelon Cells |
| title_full | Plant Hormone Signals Mediate Melatonin Synthesis to Enhance Osmotic Stress Tolerance in Watermelon Cells |
| title_fullStr | Plant Hormone Signals Mediate Melatonin Synthesis to Enhance Osmotic Stress Tolerance in Watermelon Cells |
| title_full_unstemmed | Plant Hormone Signals Mediate Melatonin Synthesis to Enhance Osmotic Stress Tolerance in Watermelon Cells |
| title_short | Plant Hormone Signals Mediate Melatonin Synthesis to Enhance Osmotic Stress Tolerance in Watermelon Cells |
| title_sort | plant hormone signals mediate melatonin synthesis to enhance osmotic stress tolerance in watermelon cells |
| topic | <i>Citrullus lanatus</i> osmotic stress melatonin plant hormone signals gene expression |
| url | https://www.mdpi.com/2311-7524/9/8/927 |
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