Melanocyte Transformation Associated with Substrate Adhesion Impediment
Exclude experimental models of malignant transformation employ chemical and physical carcinogens or genetic manipulations to study tumor progression. In this work, different melanoma cell lines were established after submitting a nontumorigenic melanocyte lineage (melan-a) to sequential cycles of fo...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2006-03-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558606800527 |
| _version_ | 1811318597348229120 |
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| author | Sueli M. Oba-Shinjo Mariangela Correa Tatiana I. Ricca Femanda Molognoni Maria A. Pinhal Izabel A. Neves Sueli K. Marie Lúcia O. Sampaio Helena B. Nader Roger Chammas Miriam G. Jasiulionis |
| author_facet | Sueli M. Oba-Shinjo Mariangela Correa Tatiana I. Ricca Femanda Molognoni Maria A. Pinhal Izabel A. Neves Sueli K. Marie Lúcia O. Sampaio Helena B. Nader Roger Chammas Miriam G. Jasiulionis |
| author_sort | Sueli M. Oba-Shinjo |
| collection | DOAJ |
| description | Exclude experimental models of malignant transformation employ chemical and physical carcinogens or genetic manipulations to study tumor progression. In this work, different melanoma cell lines were established after submitting a nontumorigenic melanocyte lineage (melan-a) to sequential cycles of forced anchorage impediment. The great majority of these cells underwent anoikis when maintained in suspension. After one deadhesion cycle, phenotypic alterations were noticeable in the few surviving cells, which became more numerous and showed progressive alterations after each adhesion impediment step. No significant differences in cell surface expression of integrins were detected, but a clear electrophoretic migration shift, compatible with an altered glycosylation pattern, was observed for β1 chain in transformed cell lines. In parallel, a progressive enrichment of tri- and tetra-antennary N-glycans was apparent, suggesting increased N-acetylglucosaminyl-transferase V activity. Alterations both in proteoglycan glycosylation pattern and core protein expression were detected during the transformation process. In conclusion, this model corroborates the role of adhesion state as a promoting agent in transformation process and demonstrates that cell adhesion disturbances may act as carcinogenic stimuli, at least for a nontumorigenic immortalized melanocyte lineage. These findings have intriguing implications for in vivo carcinogenesis, suggesting that anchorage independence may precede, and contribute to, neoplastic conversion. |
| first_indexed | 2024-04-13T12:28:36Z |
| format | Article |
| id | doaj.art-15756a63f35c4fe88ea1e268a34bca77 |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-04-13T12:28:36Z |
| publishDate | 2006-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-15756a63f35c4fe88ea1e268a34bca772022-12-22T02:46:55ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022006-03-018323124110.1593/neo.05781Melanocyte Transformation Associated with Substrate Adhesion ImpedimentSueli M. Oba-Shinjo0Mariangela Correa1Tatiana I. Ricca2Femanda Molognoni3Maria A. Pinhal4Izabel A. Neves5Sueli K. Marie6Lúcia O. Sampaio7Helena B. Nader8Roger Chammas9Miriam G. Jasiulionis10Laboratório de Biologia Molecular, Departamento de Neurologia, Faculdade de Medicina, Universidade de Săo Paulo, Săo Paulo, BrazilDisciplina de Imunologia, Departamento de Micro, Imuno e Parasitologia, Universidade Federal de Săo Paulo, Săo Paulo, BrazilDisciplina de Imunologia, Departamento de Micro, Imuno e Parasitologia, Universidade Federal de Săo Paulo, Săo Paulo, BrazilDisciplina de Imunologia, Departamento de Micro, Imuno e Parasitologia, Universidade Federal de Săo Paulo, Săo Paulo, BrazilDisciplina de Biologia Molecular, Departamento de Bioquímica, Universidade Federal de Săo Paulo, Săo Paulo, BrazilDisciplina de Biologia Molecular, Departamento de Bioquímica, Universidade Federal de Săo Paulo, Săo Paulo, BrazilLaboratório de Biologia Molecular, Departamento de Neurologia, Faculdade de Medicina, Universidade de Săo Paulo, Săo Paulo, BrazilDisciplina de Biologia Molecular, Departamento de Bioquímica, Universidade Federal de Săo Paulo, Săo Paulo, BrazilDisciplina de Biologia Molecular, Departamento de Bioquímica, Universidade Federal de Săo Paulo, Săo Paulo, BrazilLaboratorio de Oncologia Experimental, Faculdade de Medicina, Universidade de Săo Paulo, Săo Paulo, BrazilDisciplina de Imunologia, Departamento de Micro, Imuno e Parasitologia, Universidade Federal de Săo Paulo, Săo Paulo, BrazilExclude experimental models of malignant transformation employ chemical and physical carcinogens or genetic manipulations to study tumor progression. In this work, different melanoma cell lines were established after submitting a nontumorigenic melanocyte lineage (melan-a) to sequential cycles of forced anchorage impediment. The great majority of these cells underwent anoikis when maintained in suspension. After one deadhesion cycle, phenotypic alterations were noticeable in the few surviving cells, which became more numerous and showed progressive alterations after each adhesion impediment step. No significant differences in cell surface expression of integrins were detected, but a clear electrophoretic migration shift, compatible with an altered glycosylation pattern, was observed for β1 chain in transformed cell lines. In parallel, a progressive enrichment of tri- and tetra-antennary N-glycans was apparent, suggesting increased N-acetylglucosaminyl-transferase V activity. Alterations both in proteoglycan glycosylation pattern and core protein expression were detected during the transformation process. In conclusion, this model corroborates the role of adhesion state as a promoting agent in transformation process and demonstrates that cell adhesion disturbances may act as carcinogenic stimuli, at least for a nontumorigenic immortalized melanocyte lineage. These findings have intriguing implications for in vivo carcinogenesis, suggesting that anchorage independence may precede, and contribute to, neoplastic conversion.http://www.sciencedirect.com/science/article/pii/S1476558606800527Melanocyte transformationsubstrate adhesion impedimentadhesion moleculesN-glycansproteoglycans |
| spellingShingle | Sueli M. Oba-Shinjo Mariangela Correa Tatiana I. Ricca Femanda Molognoni Maria A. Pinhal Izabel A. Neves Sueli K. Marie Lúcia O. Sampaio Helena B. Nader Roger Chammas Miriam G. Jasiulionis Melanocyte Transformation Associated with Substrate Adhesion Impediment Neoplasia: An International Journal for Oncology Research Melanocyte transformation substrate adhesion impediment adhesion molecules N-glycans proteoglycans |
| title | Melanocyte Transformation Associated with Substrate Adhesion Impediment |
| title_full | Melanocyte Transformation Associated with Substrate Adhesion Impediment |
| title_fullStr | Melanocyte Transformation Associated with Substrate Adhesion Impediment |
| title_full_unstemmed | Melanocyte Transformation Associated with Substrate Adhesion Impediment |
| title_short | Melanocyte Transformation Associated with Substrate Adhesion Impediment |
| title_sort | melanocyte transformation associated with substrate adhesion impediment |
| topic | Melanocyte transformation substrate adhesion impediment adhesion molecules N-glycans proteoglycans |
| url | http://www.sciencedirect.com/science/article/pii/S1476558606800527 |
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