<i>VaCRK2</i> Mediates Gray Mold Resistance in <i>Vitis amurensis</i> by Activating the Jasmonate Signaling Pathway
Cysteine-rich receptor-like kinases (CRKs) are ubiquitous plant receptor-like kinases, which play a significant role in plant disease resistance. Gray mold is an economically important disease of grapes caused by <i>Botrytis cinerea</i>. However, <i>CRK</i> genes and their fu...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/8/1672 |
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| author | Tinggang Li Huanhuan Gao Xiaoning Tang Dongying Gong |
| author_facet | Tinggang Li Huanhuan Gao Xiaoning Tang Dongying Gong |
| author_sort | Tinggang Li |
| collection | DOAJ |
| description | Cysteine-rich receptor-like kinases (CRKs) are ubiquitous plant receptor-like kinases, which play a significant role in plant disease resistance. Gray mold is an economically important disease of grapes caused by <i>Botrytis cinerea</i>. However, <i>CRK</i> genes and their function in gray mold disease resistance in grapes have not been elucidated. This study aimed to identify and characterize <i>CRKs</i> in grapes and determine their role in gray mold resistance. Four <i>CRKs</i> were identified in <i>Vitis amurensis</i> and named <i>VaCRK1</i>–<i>VaCRK4</i> according to their genomic distribution. The four <i>VaCRKs</i> were ectopically expressed in <i>Arabidopsis thaliana</i> to study their function in defense response against <i>B. cinerea</i>. Heterologous expression of <i>VaCRK2</i> in <i>A. thaliana</i> conferred resistance to <i>B. cinerea</i>. <i>VaCRK2</i> expression in gray mold-resistant grape cultivar increased significantly after <i>B. cinerea</i> inoculation and methyl jasmonate treatment. Furthermore, the expression of jasmonic acid (JA) signaling pathway-related genes in <i>VaCRK2</i> overexpression lines of <i>A. thaliana</i> was significantly increased after <i>B. cinerea</i> inoculation, leading to the upregulation of pathogenesis-related (PR) genes and reactive oxygen species (ROS) accumulation. Overall, these results suggest that <i>VaCRK2</i> confers resistance to <i>B. cinerea</i> by activating PR gene expression and oxidative burst through the JA signaling pathway. |
| first_indexed | 2024-03-10T09:04:57Z |
| format | Article |
| id | doaj.art-157c19012e0f41b1ae39b80d81fe6551 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T09:04:57Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-157c19012e0f41b1ae39b80d81fe65512023-11-22T06:27:21ZengMDPI AGAgronomy2073-43952021-08-01118167210.3390/agronomy11081672<i>VaCRK2</i> Mediates Gray Mold Resistance in <i>Vitis amurensis</i> by Activating the Jasmonate Signaling PathwayTinggang Li0Huanhuan Gao1Xiaoning Tang2Dongying Gong3Shandong Academy of Grape, Shandong Academy of Agricultural Sciences, No. 1–27, Shanda South Road, Jinan 250100, ChinaShandong Academy of Grape, Shandong Academy of Agricultural Sciences, No. 1–27, Shanda South Road, Jinan 250100, ChinaShandong Academy of Grape, Shandong Academy of Agricultural Sciences, No. 1–27, Shanda South Road, Jinan 250100, ChinaInstitute of Agro-Food Sciences and Technology, Shandong Academy of Agricultural Sciences, No. 202, Gongye North Road, Jinan 250100, ChinaCysteine-rich receptor-like kinases (CRKs) are ubiquitous plant receptor-like kinases, which play a significant role in plant disease resistance. Gray mold is an economically important disease of grapes caused by <i>Botrytis cinerea</i>. However, <i>CRK</i> genes and their function in gray mold disease resistance in grapes have not been elucidated. This study aimed to identify and characterize <i>CRKs</i> in grapes and determine their role in gray mold resistance. Four <i>CRKs</i> were identified in <i>Vitis amurensis</i> and named <i>VaCRK1</i>–<i>VaCRK4</i> according to their genomic distribution. The four <i>VaCRKs</i> were ectopically expressed in <i>Arabidopsis thaliana</i> to study their function in defense response against <i>B. cinerea</i>. Heterologous expression of <i>VaCRK2</i> in <i>A. thaliana</i> conferred resistance to <i>B. cinerea</i>. <i>VaCRK2</i> expression in gray mold-resistant grape cultivar increased significantly after <i>B. cinerea</i> inoculation and methyl jasmonate treatment. Furthermore, the expression of jasmonic acid (JA) signaling pathway-related genes in <i>VaCRK2</i> overexpression lines of <i>A. thaliana</i> was significantly increased after <i>B. cinerea</i> inoculation, leading to the upregulation of pathogenesis-related (PR) genes and reactive oxygen species (ROS) accumulation. Overall, these results suggest that <i>VaCRK2</i> confers resistance to <i>B. cinerea</i> by activating PR gene expression and oxidative burst through the JA signaling pathway.https://www.mdpi.com/2073-4395/11/8/1672grapegray moldjasmonic acidreactive oxygen speciespathogenesis-related gene |
| spellingShingle | Tinggang Li Huanhuan Gao Xiaoning Tang Dongying Gong <i>VaCRK2</i> Mediates Gray Mold Resistance in <i>Vitis amurensis</i> by Activating the Jasmonate Signaling Pathway Agronomy grape gray mold jasmonic acid reactive oxygen species pathogenesis-related gene |
| title | <i>VaCRK2</i> Mediates Gray Mold Resistance in <i>Vitis amurensis</i> by Activating the Jasmonate Signaling Pathway |
| title_full | <i>VaCRK2</i> Mediates Gray Mold Resistance in <i>Vitis amurensis</i> by Activating the Jasmonate Signaling Pathway |
| title_fullStr | <i>VaCRK2</i> Mediates Gray Mold Resistance in <i>Vitis amurensis</i> by Activating the Jasmonate Signaling Pathway |
| title_full_unstemmed | <i>VaCRK2</i> Mediates Gray Mold Resistance in <i>Vitis amurensis</i> by Activating the Jasmonate Signaling Pathway |
| title_short | <i>VaCRK2</i> Mediates Gray Mold Resistance in <i>Vitis amurensis</i> by Activating the Jasmonate Signaling Pathway |
| title_sort | i vacrk2 i mediates gray mold resistance in i vitis amurensis i by activating the jasmonate signaling pathway |
| topic | grape gray mold jasmonic acid reactive oxygen species pathogenesis-related gene |
| url | https://www.mdpi.com/2073-4395/11/8/1672 |
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