Cholesterol-Modified Amino-Pullulan Nanoparticles as a Drug Carrier: Comparative Study of Cholesterol-Modified Carboxyethyl Pullulan and Pullulan Nanoparticles
To search for nano-drug preparations with high efficiency in tumor treatment, we evaluated the drug-loading capacity and cell-uptake toxicity of three kinds of nanoparticles (NPs). Pullulan was grafted with ethylenediamine and hydrophobic groups to form hydrophobic cholesterol-modified amino-pullula...
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| Format: | Article |
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MDPI AG
2016-09-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/6/9/165 |
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| author | Xiaojun Tao Yongchao Xie Qiufang Zhang Ximin Qiu Liming Yuan Yi Wen Min Li Xiaoping Yang Ting Tao Minghui Xie Yanwei Lv Qinyi Wang Xing Feng |
| author_facet | Xiaojun Tao Yongchao Xie Qiufang Zhang Ximin Qiu Liming Yuan Yi Wen Min Li Xiaoping Yang Ting Tao Minghui Xie Yanwei Lv Qinyi Wang Xing Feng |
| author_sort | Xiaojun Tao |
| collection | DOAJ |
| description | To search for nano-drug preparations with high efficiency in tumor treatment, we evaluated the drug-loading capacity and cell-uptake toxicity of three kinds of nanoparticles (NPs). Pullulan was grafted with ethylenediamine and hydrophobic groups to form hydrophobic cholesterol-modified amino-pullulan (CHAP) conjugates. Fourier transform infrared spectroscopy and nuclear magnetic resonance were used to identify the CHAP structure and calculate the degree of substitution of the cholesterol group. We compared three types of NPs with close cholesterol hydrophobic properties: CHAP, cholesterol-modified pullulan (CHP), and cholesterol-modified carboxylethylpullulan (CHCP), with the degree of substitution of cholesterol of 2.92%, 3.11%, and 3.46%, respectively. As compared with the two other NPs, CHAP NPs were larger, 263.9 nm, and had a positive surface charge of 7.22 mV by dynamic light-scattering measurement. CHAP NPs showed low drug-loading capacity, 12.3%, and encapsulation efficiency of 70.8%, which depended on NP hydrophobicity and was affected by surface charge. The drug release amounts of all NPs increased in the acid media, with CHAP NPs showing drug-release sensitivity with acid change. Cytotoxicity of HeLa cells was highest with mitoxantrone-loaded CHAP NPs on MTT assay. CHAP NPs may have potential as a high-efficiency drug carrier for tumor treatment. |
| first_indexed | 2024-12-14T05:33:06Z |
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| id | doaj.art-157f282d7fb048f8b391e2681d69e899 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-14T05:33:06Z |
| publishDate | 2016-09-01 |
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| series | Nanomaterials |
| spelling | doaj.art-157f282d7fb048f8b391e2681d69e8992022-12-21T23:15:17ZengMDPI AGNanomaterials2079-49912016-09-016916510.3390/nano6090165nano6090165Cholesterol-Modified Amino-Pullulan Nanoparticles as a Drug Carrier: Comparative Study of Cholesterol-Modified Carboxyethyl Pullulan and Pullulan NanoparticlesXiaojun Tao0Yongchao Xie1Qiufang Zhang2Ximin Qiu3Liming Yuan4Yi Wen5Min Li6Xiaoping Yang7Ting Tao8Minghui Xie9Yanwei Lv10Qinyi Wang11Xing Feng12Department of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacology, Hubei University of Medicine, Shiyan 442000, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaDepartment of Pharmacy, School of Medicine, Hunan Normal University, Changsha 410013, ChinaTo search for nano-drug preparations with high efficiency in tumor treatment, we evaluated the drug-loading capacity and cell-uptake toxicity of three kinds of nanoparticles (NPs). Pullulan was grafted with ethylenediamine and hydrophobic groups to form hydrophobic cholesterol-modified amino-pullulan (CHAP) conjugates. Fourier transform infrared spectroscopy and nuclear magnetic resonance were used to identify the CHAP structure and calculate the degree of substitution of the cholesterol group. We compared three types of NPs with close cholesterol hydrophobic properties: CHAP, cholesterol-modified pullulan (CHP), and cholesterol-modified carboxylethylpullulan (CHCP), with the degree of substitution of cholesterol of 2.92%, 3.11%, and 3.46%, respectively. As compared with the two other NPs, CHAP NPs were larger, 263.9 nm, and had a positive surface charge of 7.22 mV by dynamic light-scattering measurement. CHAP NPs showed low drug-loading capacity, 12.3%, and encapsulation efficiency of 70.8%, which depended on NP hydrophobicity and was affected by surface charge. The drug release amounts of all NPs increased in the acid media, with CHAP NPs showing drug-release sensitivity with acid change. Cytotoxicity of HeLa cells was highest with mitoxantrone-loaded CHAP NPs on MTT assay. CHAP NPs may have potential as a high-efficiency drug carrier for tumor treatment.http://www.mdpi.com/2079-4991/6/9/165amino pullulandegree of substitutionsurface chargedrug releasecytotoxicity |
| spellingShingle | Xiaojun Tao Yongchao Xie Qiufang Zhang Ximin Qiu Liming Yuan Yi Wen Min Li Xiaoping Yang Ting Tao Minghui Xie Yanwei Lv Qinyi Wang Xing Feng Cholesterol-Modified Amino-Pullulan Nanoparticles as a Drug Carrier: Comparative Study of Cholesterol-Modified Carboxyethyl Pullulan and Pullulan Nanoparticles Nanomaterials amino pullulan degree of substitution surface charge drug release cytotoxicity |
| title | Cholesterol-Modified Amino-Pullulan Nanoparticles as a Drug Carrier: Comparative Study of Cholesterol-Modified Carboxyethyl Pullulan and Pullulan Nanoparticles |
| title_full | Cholesterol-Modified Amino-Pullulan Nanoparticles as a Drug Carrier: Comparative Study of Cholesterol-Modified Carboxyethyl Pullulan and Pullulan Nanoparticles |
| title_fullStr | Cholesterol-Modified Amino-Pullulan Nanoparticles as a Drug Carrier: Comparative Study of Cholesterol-Modified Carboxyethyl Pullulan and Pullulan Nanoparticles |
| title_full_unstemmed | Cholesterol-Modified Amino-Pullulan Nanoparticles as a Drug Carrier: Comparative Study of Cholesterol-Modified Carboxyethyl Pullulan and Pullulan Nanoparticles |
| title_short | Cholesterol-Modified Amino-Pullulan Nanoparticles as a Drug Carrier: Comparative Study of Cholesterol-Modified Carboxyethyl Pullulan and Pullulan Nanoparticles |
| title_sort | cholesterol modified amino pullulan nanoparticles as a drug carrier comparative study of cholesterol modified carboxyethyl pullulan and pullulan nanoparticles |
| topic | amino pullulan degree of substitution surface charge drug release cytotoxicity |
| url | http://www.mdpi.com/2079-4991/6/9/165 |
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