TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-catenin
Abstract Background The urgent problem in the treatment of breast cancer is the recurrence induced by breast cancer stem cells (CSCs). Understanding the role and molecular mechanism of specific molecules in breast cancer stem cells can provide a theoretical basis for better treatment. TRIP6 is an ad...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-02-01
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| Series: | Cancer Cell International |
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| Online Access: | https://doi.org/10.1186/s12935-020-1136-z |
| _version_ | 1819055001978273792 |
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| author | Xiaohui Zhao Chao Jiang Rui Xu Qingnan Liu Guanglin Liu Yan Zhang |
| author_facet | Xiaohui Zhao Chao Jiang Rui Xu Qingnan Liu Guanglin Liu Yan Zhang |
| author_sort | Xiaohui Zhao |
| collection | DOAJ |
| description | Abstract Background The urgent problem in the treatment of breast cancer is the recurrence induced by breast cancer stem cells (CSCs). Understanding the role and molecular mechanism of specific molecules in breast cancer stem cells can provide a theoretical basis for better treatment. TRIP6 is an adapter protein which belongs to the zyxin family of LIM proteins and is important in regulating the functions of CSCs. The present study aims to investigate the effects and mechanism of TRIP6 in breast cancer. Methods TRIP6 expression in breast cancer cells and tissues were detected by Real-Time PCR, western blot and immunohistochemistry (IHC). MTT assays, colony formation assays, Xenografted tumor model and mammosphere formation assays were performed to investigate the oncogenic functions of TRIP6 in the tumorigenic capability and the tumor-initiating cell-like phenotype of breast cancer cells in vitro and in vivo. Luciferase reporter, subcellular fractionation and immunofluorescence staining assays were performed to determine the underlying mechanism of TRIP6-mediated stemness of breast cancer cells. Results TRIP6 expression was significantly upregulated in breast cancer, and was closely related to the clinicopathologic characteristics, poor overall survival (OS), relapse-free survival (RFS) and poor prognosis of breast cancer patients. Functional studies revealed that overexpression of TRIP6 significantly enhanced proliferative, tumorigenicity capability and the cancer stem cell-like properties of breast cancer in vitro and in vivo. On the contrary, silencing TRIP6 achieved the opposite results. Notably, we found that TRIP6 promoted Wnt/β-catenin signaling pathway in breast cancer to strengthen the tumor-initiating cell-like phenotype of breast cancer cells. Conclusions This study indicates that TRIP6 plays an important role in maintaining the stem cell-like characteristics of breast cancer cells, supporting the significance of TRIP6 as a novel potential prognostic biomarker and therapeutic target for diagnosis and treatment of breast cancer. |
| first_indexed | 2024-12-21T13:00:35Z |
| format | Article |
| id | doaj.art-157f71962b7c4c44a4e22ff5bf2e24a2 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-21T13:00:35Z |
| publishDate | 2020-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-157f71962b7c4c44a4e22ff5bf2e24a22022-12-21T19:03:11ZengBMCCancer Cell International1475-28672020-02-0120111110.1186/s12935-020-1136-zTRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-cateninXiaohui Zhao0Chao Jiang1Rui Xu2Qingnan Liu3Guanglin Liu4Yan Zhang5GMU-Joint School of Life Sciences, Guangzhou Medical UniversityDepartment of Cancer Center, People’s Hospital of Baoan DistrictAffiliated Cancer Hospital & Institute of Guangzhou Medical UniversityGMU-Joint School of Life Sciences, Guangzhou Medical UniversityNovartis Oncology (China) AGDepartment of Medicine Oncology, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen UniversityAbstract Background The urgent problem in the treatment of breast cancer is the recurrence induced by breast cancer stem cells (CSCs). Understanding the role and molecular mechanism of specific molecules in breast cancer stem cells can provide a theoretical basis for better treatment. TRIP6 is an adapter protein which belongs to the zyxin family of LIM proteins and is important in regulating the functions of CSCs. The present study aims to investigate the effects and mechanism of TRIP6 in breast cancer. Methods TRIP6 expression in breast cancer cells and tissues were detected by Real-Time PCR, western blot and immunohistochemistry (IHC). MTT assays, colony formation assays, Xenografted tumor model and mammosphere formation assays were performed to investigate the oncogenic functions of TRIP6 in the tumorigenic capability and the tumor-initiating cell-like phenotype of breast cancer cells in vitro and in vivo. Luciferase reporter, subcellular fractionation and immunofluorescence staining assays were performed to determine the underlying mechanism of TRIP6-mediated stemness of breast cancer cells. Results TRIP6 expression was significantly upregulated in breast cancer, and was closely related to the clinicopathologic characteristics, poor overall survival (OS), relapse-free survival (RFS) and poor prognosis of breast cancer patients. Functional studies revealed that overexpression of TRIP6 significantly enhanced proliferative, tumorigenicity capability and the cancer stem cell-like properties of breast cancer in vitro and in vivo. On the contrary, silencing TRIP6 achieved the opposite results. Notably, we found that TRIP6 promoted Wnt/β-catenin signaling pathway in breast cancer to strengthen the tumor-initiating cell-like phenotype of breast cancer cells. Conclusions This study indicates that TRIP6 plays an important role in maintaining the stem cell-like characteristics of breast cancer cells, supporting the significance of TRIP6 as a novel potential prognostic biomarker and therapeutic target for diagnosis and treatment of breast cancer.https://doi.org/10.1186/s12935-020-1136-zBreast cancerCancer stem cellsTRIP6PrognosisWnt/β-catenin |
| spellingShingle | Xiaohui Zhao Chao Jiang Rui Xu Qingnan Liu Guanglin Liu Yan Zhang TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-catenin Cancer Cell International Breast cancer Cancer stem cells TRIP6 Prognosis Wnt/β-catenin |
| title | TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-catenin |
| title_full | TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-catenin |
| title_fullStr | TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-catenin |
| title_full_unstemmed | TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-catenin |
| title_short | TRIP6 enhances stemness property of breast cancer cells through activation of Wnt/β-catenin |
| title_sort | trip6 enhances stemness property of breast cancer cells through activation of wnt β catenin |
| topic | Breast cancer Cancer stem cells TRIP6 Prognosis Wnt/β-catenin |
| url | https://doi.org/10.1186/s12935-020-1136-z |
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