Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt tolerance
Background Soil salinity is a major environmental stress that restricts crop growth and yield. Methods Here, crucial proteins and biological pathways were investigated under salt-stress and recovery conditions in Tritipyrum ‘Y1805’ using the data-independent acquisition proteomics techniques to expl...
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PeerJ Inc.
2021-12-01
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| author | Rui Yang Zhifen Yang Ze Peng Fang He Luxi Shi Yabing Dong Mingjian Ren Qingqin Zhang Guangdong Geng Suqin Zhang |
| author_facet | Rui Yang Zhifen Yang Ze Peng Fang He Luxi Shi Yabing Dong Mingjian Ren Qingqin Zhang Guangdong Geng Suqin Zhang |
| author_sort | Rui Yang |
| collection | DOAJ |
| description | Background Soil salinity is a major environmental stress that restricts crop growth and yield. Methods Here, crucial proteins and biological pathways were investigated under salt-stress and recovery conditions in Tritipyrum ‘Y1805’ using the data-independent acquisition proteomics techniques to explore its salt-tolerance mechanism. Results In total, 44 and 102 differentially expressed proteins (DEPs) were identified in ‘Y1805’ under salt-stress and recovery conditions, respectively. A proteome-transcriptome-associated analysis revealed that the expression patterns of 13 and 25 DEPs were the same under salt-stress and recovery conditions, respectively. ‘Response to stimulus’, ‘antioxidant activity’, ‘carbohydrate metabolism’, ‘amino acid metabolism’, ‘signal transduction’, ‘transport and catabolism’ and ‘biosynthesis of other secondary metabolites’ were present under both conditions in ‘Y1805’. In addition, ‘energy metabolism’ and ‘lipid metabolism’ were recovery-specific pathways, while ‘antioxidant activity’, and ‘molecular function regulator’ under salt-stress conditions, and ‘virion’ and ‘virion part’ during recovery, were ‘Y1805’-specific compared with the salt-sensitive wheat ‘Chinese Spring’. ‘Y1805’ contained eight specific DEPs related to salt-stress responses. The strong salt tolerance of ‘Y1805’ could be attributed to the strengthened cell walls, reactive oxygen species scavenging, osmoregulation, phytohormone regulation, transient growth arrest, enhanced respiration, transcriptional regulation and error information processing. These data will facilitate an understanding of the molecular mechanisms of salt tolerance and aid in the breeding of salt-tolerant wheat. |
| first_indexed | 2024-03-09T07:23:37Z |
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| id | doaj.art-157fc8662f1d427a80be9e39caaef448 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:23:37Z |
| publishDate | 2021-12-01 |
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| spelling | doaj.art-157fc8662f1d427a80be9e39caaef4482023-12-03T07:09:14ZengPeerJ Inc.PeerJ2167-83592021-12-019e1268310.7717/peerj.12683Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt toleranceRui Yang0Zhifen Yang1Ze Peng2Fang He3Luxi Shi4Yabing Dong5Mingjian Ren6Qingqin Zhang7Guangdong Geng8Suqin Zhang9Guizhou University, Guiyang, ChinaGuizhou University, Guiyang, ChinaGuizhou University, Guiyang, ChinaGuizhou University, Guiyang, ChinaGuizhou University, Guiyang, ChinaGuizhou University, Guiyang, ChinaGuizhou University, Guiyang, ChinaGuizhou University, Guiyang, ChinaGuizhou University, Guiyang, ChinaGuizhou University, Guiyang, ChinaBackground Soil salinity is a major environmental stress that restricts crop growth and yield. Methods Here, crucial proteins and biological pathways were investigated under salt-stress and recovery conditions in Tritipyrum ‘Y1805’ using the data-independent acquisition proteomics techniques to explore its salt-tolerance mechanism. Results In total, 44 and 102 differentially expressed proteins (DEPs) were identified in ‘Y1805’ under salt-stress and recovery conditions, respectively. A proteome-transcriptome-associated analysis revealed that the expression patterns of 13 and 25 DEPs were the same under salt-stress and recovery conditions, respectively. ‘Response to stimulus’, ‘antioxidant activity’, ‘carbohydrate metabolism’, ‘amino acid metabolism’, ‘signal transduction’, ‘transport and catabolism’ and ‘biosynthesis of other secondary metabolites’ were present under both conditions in ‘Y1805’. In addition, ‘energy metabolism’ and ‘lipid metabolism’ were recovery-specific pathways, while ‘antioxidant activity’, and ‘molecular function regulator’ under salt-stress conditions, and ‘virion’ and ‘virion part’ during recovery, were ‘Y1805’-specific compared with the salt-sensitive wheat ‘Chinese Spring’. ‘Y1805’ contained eight specific DEPs related to salt-stress responses. The strong salt tolerance of ‘Y1805’ could be attributed to the strengthened cell walls, reactive oxygen species scavenging, osmoregulation, phytohormone regulation, transient growth arrest, enhanced respiration, transcriptional regulation and error information processing. These data will facilitate an understanding of the molecular mechanisms of salt tolerance and aid in the breeding of salt-tolerant wheat.https://peerj.com/articles/12683.pdfTritipyrumSalt stressRecoveryProteomeTranscriptomeCrucial proteins and pathways |
| spellingShingle | Rui Yang Zhifen Yang Ze Peng Fang He Luxi Shi Yabing Dong Mingjian Ren Qingqin Zhang Guangdong Geng Suqin Zhang Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt tolerance PeerJ Tritipyrum Salt stress Recovery Proteome Transcriptome Crucial proteins and pathways |
| title | Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt tolerance |
| title_full | Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt tolerance |
| title_fullStr | Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt tolerance |
| title_full_unstemmed | Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt tolerance |
| title_short | Integrated transcriptomic and proteomic analysis of Tritipyrum provides insights into the molecular basis of salt tolerance |
| title_sort | integrated transcriptomic and proteomic analysis of tritipyrum provides insights into the molecular basis of salt tolerance |
| topic | Tritipyrum Salt stress Recovery Proteome Transcriptome Crucial proteins and pathways |
| url | https://peerj.com/articles/12683.pdf |
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