Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs
Archaeal viruses are one of the most mysterious parts of the virosphere because of their diverse morphologies and unique genome contents. The crenarchaeal viruses are commonly found in high temperature and acidic hot springs, and the number of identified crenarchaeal viruses is being rapidly increas...
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| Format: | Article |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1134935/full |
| _version_ | 1797855860155744256 |
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| author | Xi Feng Yanan Li Chang Tian Wei Yang Xinyu Liu Changyi Zhang Zhirui Zeng Zhirui Zeng |
| author_facet | Xi Feng Yanan Li Chang Tian Wei Yang Xinyu Liu Changyi Zhang Zhirui Zeng Zhirui Zeng |
| author_sort | Xi Feng |
| collection | DOAJ |
| description | Archaeal viruses are one of the most mysterious parts of the virosphere because of their diverse morphologies and unique genome contents. The crenarchaeal viruses are commonly found in high temperature and acidic hot springs, and the number of identified crenarchaeal viruses is being rapidly increased in recent two decades. Over fifty viruses infecting the members of the order Sulfolobales have been identified, most of which are from hot springs distributed in the United States, Russia, Iceland, Japan, and Italy. To further expand the reservoir of viruses infecting strains of Sulfolobaceae, we investigated virus diversity through cultivation-dependent approaches in hot springs in Tengchong, Yunnan, China. Eight different virus-like particles were detected in enrichment cultures, among which five new archaeal viruses were isolated and characterized. We showed that these viruses can infect acidophilic hyperthermophiles belonging to three different genera of the family Sulfolobaceae, namely, Saccharolobus, Sulfolobus, and Metallosphaera. We also compared the lipid compositions of the viral and cellular membranes and found that the lipid composition of some viral envelopes was very different from that of the host membrane. Collectively, our results showed that the Tengchong hot springs harbor highly diverse viruses, providing excellent models for archaeal virus-host studies. |
| first_indexed | 2024-04-09T20:30:44Z |
| format | Article |
| id | doaj.art-1581e9a7d59d40d28f58f4337aaace40 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T20:30:44Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1581e9a7d59d40d28f58f4337aaace402023-03-30T16:36:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.11349351134935Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springsXi Feng0Yanan Li1Chang Tian2Wei Yang3Xinyu Liu4Changyi Zhang5Zhirui Zeng6Zhirui Zeng7Department of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, ChinaDepartment of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, ChinaDepartment of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, ChinaDepartment of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, ChinaDepartment of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, ChinaCarl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United StatesDepartment of Ocean Science and Engineering, Southern University of Science and Technology, Shenzhen, ChinaSouthern Marine Science and Engineering Guangdong Laboratory, Guangzhou, ChinaArchaeal viruses are one of the most mysterious parts of the virosphere because of their diverse morphologies and unique genome contents. The crenarchaeal viruses are commonly found in high temperature and acidic hot springs, and the number of identified crenarchaeal viruses is being rapidly increased in recent two decades. Over fifty viruses infecting the members of the order Sulfolobales have been identified, most of which are from hot springs distributed in the United States, Russia, Iceland, Japan, and Italy. To further expand the reservoir of viruses infecting strains of Sulfolobaceae, we investigated virus diversity through cultivation-dependent approaches in hot springs in Tengchong, Yunnan, China. Eight different virus-like particles were detected in enrichment cultures, among which five new archaeal viruses were isolated and characterized. We showed that these viruses can infect acidophilic hyperthermophiles belonging to three different genera of the family Sulfolobaceae, namely, Saccharolobus, Sulfolobus, and Metallosphaera. We also compared the lipid compositions of the viral and cellular membranes and found that the lipid composition of some viral envelopes was very different from that of the host membrane. Collectively, our results showed that the Tengchong hot springs harbor highly diverse viruses, providing excellent models for archaeal virus-host studies.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1134935/fullarchaeal virusSulfolobaceaehot springsTengchongviral membrane lipid |
| spellingShingle | Xi Feng Yanan Li Chang Tian Wei Yang Xinyu Liu Changyi Zhang Zhirui Zeng Zhirui Zeng Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs Frontiers in Microbiology archaeal virus Sulfolobaceae hot springs Tengchong viral membrane lipid |
| title | Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
| title_full | Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
| title_fullStr | Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
| title_full_unstemmed | Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
| title_short | Isolation of archaeal viruses with lipid membrane from Tengchong acidic hot springs |
| title_sort | isolation of archaeal viruses with lipid membrane from tengchong acidic hot springs |
| topic | archaeal virus Sulfolobaceae hot springs Tengchong viral membrane lipid |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1134935/full |
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