Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAs
Summary: MicroRNAs (miRNAs) play an important biomarker in various biological processes, especially cancer related, yet economic, simple, sensitive and specific methods for miRNA determination are still challenging. In this study, we have developed stepwise-strand displacement amplification (S-SDA)-...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-04-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422300408X |
| _version_ | 1797868263397392384 |
|---|---|
| author | Huo Xu Fenglin Yang Danlong Chen Weilin Ye Guohui Xue Lee Jia |
| author_facet | Huo Xu Fenglin Yang Danlong Chen Weilin Ye Guohui Xue Lee Jia |
| author_sort | Huo Xu |
| collection | DOAJ |
| description | Summary: MicroRNAs (miRNAs) play an important biomarker in various biological processes, especially cancer related, yet economic, simple, sensitive and specific methods for miRNA determination are still challenging. In this study, we have developed stepwise-strand displacement amplification (S-SDA)-based colorimetric sensing platform for let-7a miRNA detection in clinical serum samples. Our results demonstrated that the developed S-SDA-based method shows high sensitivity with a detection limit of 63.2 pM and a naked eye detection limit of 0.1 nM. Moreover, the S-SDA amplifier is able to discriminate target miRNAs from their mutants with high accuracy and specificity. With its high sensitivity and selectivity, this method successfully identified healthy individuals from patients with colon cancer by detecting let-7a miRNAs in serum. We believe the colorimetric analysis method will provide a new paradigm for the detection of miRNA with different abundance and show great potential for clinical application in biomedical analysis and early clinical diagnosis. |
| first_indexed | 2024-04-09T23:53:12Z |
| format | Article |
| id | doaj.art-158cfcc024254bb78b46f75660ea580d |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T23:53:12Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-158cfcc024254bb78b46f75660ea580d2023-03-17T04:33:43ZengElsevieriScience2589-00422023-04-01264106331Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAsHuo Xu0Fenglin Yang1Danlong Chen2Weilin Ye3Guohui Xue4Lee Jia5College of Materials and Chemical Engineering, Minjiang University, Fuzhou, Fujian, 350108, China; Corresponding authorCollege of Materials and Chemical Engineering, Minjiang University, Fuzhou, Fujian, 350108, ChinaCollege of Materials and Chemical Engineering, Minjiang University, Fuzhou, Fujian, 350108, ChinaCollege of Materials and Chemical Engineering, Minjiang University, Fuzhou, Fujian, 350108, ChinaDepartment of Clinical Laboratory, Jiujiang NO.1 People’s Hospital, Jiujiang, Jiangxi, 332000, ChinaCollege of Materials and Chemical Engineering, Minjiang University, Fuzhou, Fujian, 350108, ChinaSummary: MicroRNAs (miRNAs) play an important biomarker in various biological processes, especially cancer related, yet economic, simple, sensitive and specific methods for miRNA determination are still challenging. In this study, we have developed stepwise-strand displacement amplification (S-SDA)-based colorimetric sensing platform for let-7a miRNA detection in clinical serum samples. Our results demonstrated that the developed S-SDA-based method shows high sensitivity with a detection limit of 63.2 pM and a naked eye detection limit of 0.1 nM. Moreover, the S-SDA amplifier is able to discriminate target miRNAs from their mutants with high accuracy and specificity. With its high sensitivity and selectivity, this method successfully identified healthy individuals from patients with colon cancer by detecting let-7a miRNAs in serum. We believe the colorimetric analysis method will provide a new paradigm for the detection of miRNA with different abundance and show great potential for clinical application in biomedical analysis and early clinical diagnosis.http://www.sciencedirect.com/science/article/pii/S258900422300408XAnalytical chemistry applicationsDiagnostic technique in health technologyMedical device in health technology |
| spellingShingle | Huo Xu Fenglin Yang Danlong Chen Weilin Ye Guohui Xue Lee Jia Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAs iScience Analytical chemistry applications Diagnostic technique in health technology Medical device in health technology |
| title | Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAs |
| title_full | Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAs |
| title_fullStr | Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAs |
| title_full_unstemmed | Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAs |
| title_short | Trigging stepwise-strand displacement amplification lights up numerous G-quadruplex for colorimetric signaling of serum microRNAs |
| title_sort | trigging stepwise strand displacement amplification lights up numerous g quadruplex for colorimetric signaling of serum micrornas |
| topic | Analytical chemistry applications Diagnostic technique in health technology Medical device in health technology |
| url | http://www.sciencedirect.com/science/article/pii/S258900422300408X |
| work_keys_str_mv | AT huoxu triggingstepwisestranddisplacementamplificationlightsupnumerousgquadruplexforcolorimetricsignalingofserummicrornas AT fenglinyang triggingstepwisestranddisplacementamplificationlightsupnumerousgquadruplexforcolorimetricsignalingofserummicrornas AT danlongchen triggingstepwisestranddisplacementamplificationlightsupnumerousgquadruplexforcolorimetricsignalingofserummicrornas AT weilinye triggingstepwisestranddisplacementamplificationlightsupnumerousgquadruplexforcolorimetricsignalingofserummicrornas AT guohuixue triggingstepwisestranddisplacementamplificationlightsupnumerousgquadruplexforcolorimetricsignalingofserummicrornas AT leejia triggingstepwisestranddisplacementamplificationlightsupnumerousgquadruplexforcolorimetricsignalingofserummicrornas |