Expression of Human L-Dopa Decarboxylase (DDC) under Conditions of Oxidative Stress
Oxidative stress is known to influence mRNA levels, translation, and proteolysis. The importance of oxidative stress has been demonstrated in several human diseases, including neurodegenerative disorders. L-Dopa decarboxylase (DDC) is the enzyme that converts L-Dopa to dopamine (DA). In spite of a l...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/45/12/635 |
| _version_ | 1797381574973456384 |
|---|---|
| author | Nikolaos S. Lotsios Nikolaos Arvanitis Alexandros G. Charonitakis George Mpekoulis Efseveia Frakolaki Niki Vassilaki Diamantis C. Sideris Dido Vassilacopoulou |
| author_facet | Nikolaos S. Lotsios Nikolaos Arvanitis Alexandros G. Charonitakis George Mpekoulis Efseveia Frakolaki Niki Vassilaki Diamantis C. Sideris Dido Vassilacopoulou |
| author_sort | Nikolaos S. Lotsios |
| collection | DOAJ |
| description | Oxidative stress is known to influence mRNA levels, translation, and proteolysis. The importance of oxidative stress has been demonstrated in several human diseases, including neurodegenerative disorders. L-Dopa decarboxylase (DDC) is the enzyme that converts L-Dopa to dopamine (DA). In spite of a large number of studies, little is known about the biological significance of the enzyme under physiological and pathological conditions. Here, we investigated the relationship between DDC expression and oxidative stress in human neural and non-neural cells. Oxidative stress was induced by treatment with H<sub>2</sub>O<sub>2</sub>. Our data indicated that mRNA and protein expression of DDC was enhanced or remained stable under conditions of ROS induction, despite degradation of total RNA and increased cytotoxicity and apoptosis. Moreover, DDC silencing caused an increase in the H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity. The current study suggests that DDC is involved in the mechanisms of oxidative stress. |
| first_indexed | 2024-03-08T20:53:24Z |
| format | Article |
| id | doaj.art-158f5c096b5d478299dfe4a652b8a995 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-08T20:53:24Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-158f5c096b5d478299dfe4a652b8a9952023-12-22T14:00:46ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-12-014512101791019210.3390/cimb45120635Expression of Human L-Dopa Decarboxylase (DDC) under Conditions of Oxidative StressNikolaos S. Lotsios0Nikolaos Arvanitis1Alexandros G. Charonitakis2George Mpekoulis3Efseveia Frakolaki4Niki Vassilaki5Diamantis C. Sideris6Dido Vassilacopoulou7Section of Biochemistry and Molecular Biology, Department of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceSection of Biochemistry and Molecular Biology, Department of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceSection of Biochemistry and Molecular Biology, Department of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceLaboratory of Molecular Virology, Hellenic Pasteur Institute (HPI), 11521 Athens, GreeceLaboratory of Molecular Virology, Hellenic Pasteur Institute (HPI), 11521 Athens, GreeceLaboratory of Molecular Virology, Hellenic Pasteur Institute (HPI), 11521 Athens, GreeceSection of Biochemistry and Molecular Biology, Department of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceSection of Biochemistry and Molecular Biology, Department of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceOxidative stress is known to influence mRNA levels, translation, and proteolysis. The importance of oxidative stress has been demonstrated in several human diseases, including neurodegenerative disorders. L-Dopa decarboxylase (DDC) is the enzyme that converts L-Dopa to dopamine (DA). In spite of a large number of studies, little is known about the biological significance of the enzyme under physiological and pathological conditions. Here, we investigated the relationship between DDC expression and oxidative stress in human neural and non-neural cells. Oxidative stress was induced by treatment with H<sub>2</sub>O<sub>2</sub>. Our data indicated that mRNA and protein expression of DDC was enhanced or remained stable under conditions of ROS induction, despite degradation of total RNA and increased cytotoxicity and apoptosis. Moreover, DDC silencing caused an increase in the H<sub>2</sub>O<sub>2</sub>-induced cytotoxicity. The current study suggests that DDC is involved in the mechanisms of oxidative stress.https://www.mdpi.com/1467-3045/45/12/635L-Dopa decarboxylaseoxidative stressH<sub>2</sub>O<sub>2</sub>apoptosisneural and non-neural cells |
| spellingShingle | Nikolaos S. Lotsios Nikolaos Arvanitis Alexandros G. Charonitakis George Mpekoulis Efseveia Frakolaki Niki Vassilaki Diamantis C. Sideris Dido Vassilacopoulou Expression of Human L-Dopa Decarboxylase (DDC) under Conditions of Oxidative Stress Current Issues in Molecular Biology L-Dopa decarboxylase oxidative stress H<sub>2</sub>O<sub>2</sub> apoptosis neural and non-neural cells |
| title | Expression of Human L-Dopa Decarboxylase (DDC) under Conditions of Oxidative Stress |
| title_full | Expression of Human L-Dopa Decarboxylase (DDC) under Conditions of Oxidative Stress |
| title_fullStr | Expression of Human L-Dopa Decarboxylase (DDC) under Conditions of Oxidative Stress |
| title_full_unstemmed | Expression of Human L-Dopa Decarboxylase (DDC) under Conditions of Oxidative Stress |
| title_short | Expression of Human L-Dopa Decarboxylase (DDC) under Conditions of Oxidative Stress |
| title_sort | expression of human l dopa decarboxylase ddc under conditions of oxidative stress |
| topic | L-Dopa decarboxylase oxidative stress H<sub>2</sub>O<sub>2</sub> apoptosis neural and non-neural cells |
| url | https://www.mdpi.com/1467-3045/45/12/635 |
| work_keys_str_mv | AT nikolaosslotsios expressionofhumanldopadecarboxylaseddcunderconditionsofoxidativestress AT nikolaosarvanitis expressionofhumanldopadecarboxylaseddcunderconditionsofoxidativestress AT alexandrosgcharonitakis expressionofhumanldopadecarboxylaseddcunderconditionsofoxidativestress AT georgempekoulis expressionofhumanldopadecarboxylaseddcunderconditionsofoxidativestress AT efseveiafrakolaki expressionofhumanldopadecarboxylaseddcunderconditionsofoxidativestress AT nikivassilaki expressionofhumanldopadecarboxylaseddcunderconditionsofoxidativestress AT diamantiscsideris expressionofhumanldopadecarboxylaseddcunderconditionsofoxidativestress AT didovassilacopoulou expressionofhumanldopadecarboxylaseddcunderconditionsofoxidativestress |