Characterization, antibacterial and antibiofilm evaluation of biosynthesized silver nanoparticles from Pseudomonas aeruginosa against drug resistant Acinetobacter baumannii
Antimicrobial resistance is regarded as one of the top three terrible events threatening the worldwide existence of humans Here of, Acinetobacter baumannii evolved as the most challenging pathogen threatening to initiate the post-antibiotic era. Their ability to withstand antibiotics is attrib...
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Format: | Article |
Language: | English |
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College of Pharmacy / Mustansiriyah University
2023-07-01
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Series: | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
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Online Access: | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/1049 |
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author | Talar Ibrahim Hasan Akhter Ahmed Ahmed |
author_facet | Talar Ibrahim Hasan Akhter Ahmed Ahmed |
author_sort | Talar Ibrahim Hasan |
collection | DOAJ |
description |
Antimicrobial resistance is regarded as one of the top three terrible events threatening the worldwide existence of humans Here of, Acinetobacter baumannii evolved as the most challenging pathogen threatening to initiate the post-antibiotic era.
Their ability to withstand antibiotics is attributed to a set of virulence determinants in particular biofilms which are known to enhance pathogenesis and drug resistance potency. Studies regarding green silver nanoparticles (AgNP)s as an alternative treatment modality to antibiotics increased over recent years. Considering these facts, we aimed to explore the antibiofilm effect of AgNPs in the multi-drug-resistant Acinetobacter baumannii. AgNPs were bio-fabricated by Pseudomonas aeruginosa and characterized via FTIR, UV-Vis, XRD, EDS, and SEM. Well-diffusion was used to screen the antimicrobial effects of AgNPs. Minimal-inhibitory concentrations of AgNPs were determined to study their antibiofilm effect at sub-inhibitory concentrations (SIC). Results showed that all isolates were biofilm producers and portrayed high resistance to the tested antibiotics. Characterization results supported the successful fabrication of crystalline nanoparticles. Exposure of the isolates to the bacteriogenic AgNPs resulted in pronounced inhibition zones and reduced biofilms at SICs values. These results indicate that Pseudomonas aeruginosa can be employed to produce AgNPs with an aptitude to disrupt biofilm development and growth in the multi-drug resistant Acinetobacter baumannii.
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first_indexed | 2024-04-24T13:45:46Z |
format | Article |
id | doaj.art-159748d00ddf4b98b891ce2c085392eb |
institution | Directory Open Access Journal |
issn | 1815-0993 2959-183X |
language | English |
last_indexed | 2024-04-24T13:45:46Z |
publishDate | 2023-07-01 |
publisher | College of Pharmacy / Mustansiriyah University |
record_format | Article |
series | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
spelling | doaj.art-159748d00ddf4b98b891ce2c085392eb2024-04-04T06:20:52ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2023-07-0123310.32947/ajps.v23i3.1049Characterization, antibacterial and antibiofilm evaluation of biosynthesized silver nanoparticles from Pseudomonas aeruginosa against drug resistant Acinetobacter baumanniiTalar Ibrahim Hasan0Akhter Ahmed Ahmed1Department of Biology, College of Science, Salahaddin University- Erbil, IraqDepartment of Biology, College of Science, Salahaddin University- Erbil, Iraq Antimicrobial resistance is regarded as one of the top three terrible events threatening the worldwide existence of humans Here of, Acinetobacter baumannii evolved as the most challenging pathogen threatening to initiate the post-antibiotic era. Their ability to withstand antibiotics is attributed to a set of virulence determinants in particular biofilms which are known to enhance pathogenesis and drug resistance potency. Studies regarding green silver nanoparticles (AgNP)s as an alternative treatment modality to antibiotics increased over recent years. Considering these facts, we aimed to explore the antibiofilm effect of AgNPs in the multi-drug-resistant Acinetobacter baumannii. AgNPs were bio-fabricated by Pseudomonas aeruginosa and characterized via FTIR, UV-Vis, XRD, EDS, and SEM. Well-diffusion was used to screen the antimicrobial effects of AgNPs. Minimal-inhibitory concentrations of AgNPs were determined to study their antibiofilm effect at sub-inhibitory concentrations (SIC). Results showed that all isolates were biofilm producers and portrayed high resistance to the tested antibiotics. Characterization results supported the successful fabrication of crystalline nanoparticles. Exposure of the isolates to the bacteriogenic AgNPs resulted in pronounced inhibition zones and reduced biofilms at SICs values. These results indicate that Pseudomonas aeruginosa can be employed to produce AgNPs with an aptitude to disrupt biofilm development and growth in the multi-drug resistant Acinetobacter baumannii. https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/1049drug resistance, biofilm, Acinetobacter baumannii, biogenic silver nanoparticles |
spellingShingle | Talar Ibrahim Hasan Akhter Ahmed Ahmed Characterization, antibacterial and antibiofilm evaluation of biosynthesized silver nanoparticles from Pseudomonas aeruginosa against drug resistant Acinetobacter baumannii Al-Mustansiriyah Journal of Pharmaceutical Sciences drug resistance, biofilm, Acinetobacter baumannii, biogenic silver nanoparticles |
title | Characterization, antibacterial and antibiofilm evaluation of biosynthesized silver nanoparticles from Pseudomonas aeruginosa against drug resistant Acinetobacter baumannii |
title_full | Characterization, antibacterial and antibiofilm evaluation of biosynthesized silver nanoparticles from Pseudomonas aeruginosa against drug resistant Acinetobacter baumannii |
title_fullStr | Characterization, antibacterial and antibiofilm evaluation of biosynthesized silver nanoparticles from Pseudomonas aeruginosa against drug resistant Acinetobacter baumannii |
title_full_unstemmed | Characterization, antibacterial and antibiofilm evaluation of biosynthesized silver nanoparticles from Pseudomonas aeruginosa against drug resistant Acinetobacter baumannii |
title_short | Characterization, antibacterial and antibiofilm evaluation of biosynthesized silver nanoparticles from Pseudomonas aeruginosa against drug resistant Acinetobacter baumannii |
title_sort | characterization antibacterial and antibiofilm evaluation of biosynthesized silver nanoparticles from pseudomonas aeruginosa against drug resistant acinetobacter baumannii |
topic | drug resistance, biofilm, Acinetobacter baumannii, biogenic silver nanoparticles |
url | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/1049 |
work_keys_str_mv | AT talaribrahimhasan characterizationantibacterialandantibiofilmevaluationofbiosynthesizedsilvernanoparticlesfrompseudomonasaeruginosaagainstdrugresistantacinetobacterbaumannii AT akhterahmedahmed characterizationantibacterialandantibiofilmevaluationofbiosynthesizedsilvernanoparticlesfrompseudomonasaeruginosaagainstdrugresistantacinetobacterbaumannii |