Effects of grape seed proanthocyanidin extract on lipopolysaccharide translocation and trafficking from the gut to tissues
Diet-associated alterations of the intestinal barrier and gut microbiota promote intestinal lipopolysaccharide (LPS) translocation from the lumen to the lamina propria through different pathways, leading to an increase in LPS levels in the plasma known as metabolic endotoxemia.As a pharmacological d...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623001664 |
| _version_ | 1797819863431905280 |
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| author | Marta Sierra-Cruz Alba Miguéns-Gómez Esther Rodríguez-Gallego Claudio D'Addario Martina Di Bartolomeo M Teresa Blay Montserrat Pinent Raúl Beltrán-Debón Ximena Terra |
| author_facet | Marta Sierra-Cruz Alba Miguéns-Gómez Esther Rodríguez-Gallego Claudio D'Addario Martina Di Bartolomeo M Teresa Blay Montserrat Pinent Raúl Beltrán-Debón Ximena Terra |
| author_sort | Marta Sierra-Cruz |
| collection | DOAJ |
| description | Diet-associated alterations of the intestinal barrier and gut microbiota promote intestinal lipopolysaccharide (LPS) translocation from the lumen to the lamina propria through different pathways, leading to an increase in LPS levels in the plasma known as metabolic endotoxemia.As a pharmacological dose of grape seed proanthocyanidin extract (GSPE) can reduce metabolic endotoxemia of obese rats, in the current study, we aimed to evaluate GSPE modulation of LPS translocation and the underlying mechanisms. We performed both an in vitro experiment with Caco-2 cells and an in vivo experiment with Wistar female rats fed a cafeteria (CAF) diet. GSPE was effective in regulating intestinal permeability through the modulation of receptor-mediated endocytosis pathway, as well as the gut microbiota interaction with the endocannabinoid system through epigenetic mechanisms. Our results confirm that GSPE can ameliorate intestinal dysfunction and metabolic endotoxemia caused by an excess of dietary lipids by modulating the endotoxin-translocation pathways. |
| first_indexed | 2024-03-13T09:28:53Z |
| format | Article |
| id | doaj.art-159df53ba8c844778feec46b92e4b07c |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-13T09:28:53Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-159df53ba8c844778feec46b92e4b07c2023-05-26T04:21:12ZengElsevierJournal of Functional Foods1756-46462023-06-01105105566Effects of grape seed proanthocyanidin extract on lipopolysaccharide translocation and trafficking from the gut to tissuesMarta Sierra-Cruz0Alba Miguéns-Gómez1Esther Rodríguez-Gallego2Claudio D'Addario3Martina Di Bartolomeo4M Teresa Blay5Montserrat Pinent6Raúl Beltrán-Debón7Ximena Terra8Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, MoBioFood Research Group, Tarragona, SpainUniversitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, MoBioFood Research Group, Tarragona, SpainUniversitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, MoBioFood Research Group, Tarragona, SpainFaculty of Bioscience, University of Teramo, Teramo, ItalyFaculty of Bioscience, University of Teramo, Teramo, ItalyUniversitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, MoBioFood Research Group, Tarragona, SpainUniversitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, MoBioFood Research Group, Tarragona, SpainUniversitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, MoBioFood Research Group, Tarragona, Spain; Corresponding author.Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, MoBioFood Research Group, Tarragona, SpainDiet-associated alterations of the intestinal barrier and gut microbiota promote intestinal lipopolysaccharide (LPS) translocation from the lumen to the lamina propria through different pathways, leading to an increase in LPS levels in the plasma known as metabolic endotoxemia.As a pharmacological dose of grape seed proanthocyanidin extract (GSPE) can reduce metabolic endotoxemia of obese rats, in the current study, we aimed to evaluate GSPE modulation of LPS translocation and the underlying mechanisms. We performed both an in vitro experiment with Caco-2 cells and an in vivo experiment with Wistar female rats fed a cafeteria (CAF) diet. GSPE was effective in regulating intestinal permeability through the modulation of receptor-mediated endocytosis pathway, as well as the gut microbiota interaction with the endocannabinoid system through epigenetic mechanisms. Our results confirm that GSPE can ameliorate intestinal dysfunction and metabolic endotoxemia caused by an excess of dietary lipids by modulating the endotoxin-translocation pathways.http://www.sciencedirect.com/science/article/pii/S1756464623001664Intestinal permeabilityMetabolic endotoxemiaProanthocyanidinsGut microbiotaSystemic inflammation |
| spellingShingle | Marta Sierra-Cruz Alba Miguéns-Gómez Esther Rodríguez-Gallego Claudio D'Addario Martina Di Bartolomeo M Teresa Blay Montserrat Pinent Raúl Beltrán-Debón Ximena Terra Effects of grape seed proanthocyanidin extract on lipopolysaccharide translocation and trafficking from the gut to tissues Journal of Functional Foods Intestinal permeability Metabolic endotoxemia Proanthocyanidins Gut microbiota Systemic inflammation |
| title | Effects of grape seed proanthocyanidin extract on lipopolysaccharide translocation and trafficking from the gut to tissues |
| title_full | Effects of grape seed proanthocyanidin extract on lipopolysaccharide translocation and trafficking from the gut to tissues |
| title_fullStr | Effects of grape seed proanthocyanidin extract on lipopolysaccharide translocation and trafficking from the gut to tissues |
| title_full_unstemmed | Effects of grape seed proanthocyanidin extract on lipopolysaccharide translocation and trafficking from the gut to tissues |
| title_short | Effects of grape seed proanthocyanidin extract on lipopolysaccharide translocation and trafficking from the gut to tissues |
| title_sort | effects of grape seed proanthocyanidin extract on lipopolysaccharide translocation and trafficking from the gut to tissues |
| topic | Intestinal permeability Metabolic endotoxemia Proanthocyanidins Gut microbiota Systemic inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623001664 |
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