Self-Assembled Inkjet Printer for Droplet Digital Loop-Mediated Isothermal Amplification
Developing rapid and inexpensive diagnostic tools for molecular detection has been pushed forward by the advancements of technical aspects. However, attention has rarely been paid to the molecular detection methodology using inkjet printing technique. Herein, we developed an approach that employed a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/10/7/247 |
| _version_ | 1797406952446230528 |
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| author | Zhaoxuan Fan Yucheng Sun Jin-Ming Lin |
| author_facet | Zhaoxuan Fan Yucheng Sun Jin-Ming Lin |
| author_sort | Zhaoxuan Fan |
| collection | DOAJ |
| description | Developing rapid and inexpensive diagnostic tools for molecular detection has been pushed forward by the advancements of technical aspects. However, attention has rarely been paid to the molecular detection methodology using inkjet printing technique. Herein, we developed an approach that employed a self-assembled inkjet printer as the enabling technology to realize droplet digital loop-mediated isothermal amplification in a low-cost and practical format. An inkjet printer is a self-assembled tool for the generation of discrete droplets in controllable volumes from a picoliter to a nanoliter. A microfluidic chip serves as a droplets reservoir to perform droplet digital LAMP assays. The inkjet printer approach successfully quantified the HPV16 from CaSki cells. This self-assembled and practical inkjet printer device may therefore become a promising tool for rapid molecular detection and can be extended to on-site analysis. |
| first_indexed | 2024-03-09T03:34:13Z |
| format | Article |
| id | doaj.art-159fe506247a479fbdff6a987559d8a2 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T03:34:13Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-159fe506247a479fbdff6a987559d8a22023-12-03T14:50:15ZengMDPI AGChemosensors2227-90402022-06-0110724710.3390/chemosensors10070247Self-Assembled Inkjet Printer for Droplet Digital Loop-Mediated Isothermal AmplificationZhaoxuan Fan0Yucheng Sun1Jin-Ming Lin2Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, ChinaBeijing Key Laboratory of Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, ChinaBeijing Key Laboratory of Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, ChinaDeveloping rapid and inexpensive diagnostic tools for molecular detection has been pushed forward by the advancements of technical aspects. However, attention has rarely been paid to the molecular detection methodology using inkjet printing technique. Herein, we developed an approach that employed a self-assembled inkjet printer as the enabling technology to realize droplet digital loop-mediated isothermal amplification in a low-cost and practical format. An inkjet printer is a self-assembled tool for the generation of discrete droplets in controllable volumes from a picoliter to a nanoliter. A microfluidic chip serves as a droplets reservoir to perform droplet digital LAMP assays. The inkjet printer approach successfully quantified the HPV16 from CaSki cells. This self-assembled and practical inkjet printer device may therefore become a promising tool for rapid molecular detection and can be extended to on-site analysis.https://www.mdpi.com/2227-9040/10/7/247inkjet printerdropletsdigital LAMPmicrofluidics |
| spellingShingle | Zhaoxuan Fan Yucheng Sun Jin-Ming Lin Self-Assembled Inkjet Printer for Droplet Digital Loop-Mediated Isothermal Amplification Chemosensors inkjet printer droplets digital LAMP microfluidics |
| title | Self-Assembled Inkjet Printer for Droplet Digital Loop-Mediated Isothermal Amplification |
| title_full | Self-Assembled Inkjet Printer for Droplet Digital Loop-Mediated Isothermal Amplification |
| title_fullStr | Self-Assembled Inkjet Printer for Droplet Digital Loop-Mediated Isothermal Amplification |
| title_full_unstemmed | Self-Assembled Inkjet Printer for Droplet Digital Loop-Mediated Isothermal Amplification |
| title_short | Self-Assembled Inkjet Printer for Droplet Digital Loop-Mediated Isothermal Amplification |
| title_sort | self assembled inkjet printer for droplet digital loop mediated isothermal amplification |
| topic | inkjet printer droplets digital LAMP microfluidics |
| url | https://www.mdpi.com/2227-9040/10/7/247 |
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