Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer
Abstract Background Frozen-thawed embryo transfer (FET) is increasingly available for the improvement of the success rate of assisted reproductive technologies other than fresh embryo transfer (ET). There have been numerous findings that FET provides better obstetric and perinatal outcomes. However,...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | Clinical Epigenetics |
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| Online Access: | http://link.springer.com/article/10.1186/s13148-017-0379-6 |
| _version_ | 1828375720884174848 |
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| author | Hitoshi Hiura Hiromitsu Hattori Norio Kobayashi Hiroaki Okae Hatsune Chiba Naoko Miyauchi Akane Kitamura Hiroyuki Kikuchi Hiroaki Yoshida Takahiro Arima |
| author_facet | Hitoshi Hiura Hiromitsu Hattori Norio Kobayashi Hiroaki Okae Hatsune Chiba Naoko Miyauchi Akane Kitamura Hiroyuki Kikuchi Hiroaki Yoshida Takahiro Arima |
| author_sort | Hitoshi Hiura |
| collection | DOAJ |
| description | Abstract Background Frozen-thawed embryo transfer (FET) is increasingly available for the improvement of the success rate of assisted reproductive technologies other than fresh embryo transfer (ET). There have been numerous findings that FET provides better obstetric and perinatal outcomes. However, the birth weight of infants conceived using FET is heavier than that of those conceived via ET. In addition, some reports have suggested that FET is associated with perinatal diseases such as placenta accreta and pregnancy-induced hypertension (PIH). Results In this study, we compared the microRNA (miRNA) expression profiles in term placentae derived from FET, ET, and spontaneous pregnancy (SP). We identified four miRNAs, miR-130a-3p, miR-149-5p, miR-423-5p, and miR-487b-3p, that were significantly downregulated in FET placentae compared with those from SP and ET. We found that DNA methylation of MEG3-DMR, not but IG-DMR, was associated with miRNA expression of the DLK1-DIO3 imprinted domain in the human placenta. In functional analyses, GO terms and signaling pathways related to positive regulation of gene expression, growth, development, cell migration, and type II diabetes mellitus (T2DM) were enriched. Conclusions This study supports the hypothesis that the process of FET may increase exposure of epigenome to external influences. |
| first_indexed | 2024-04-14T07:50:31Z |
| format | Article |
| id | doaj.art-15a234c6fca4438cac257f3838e8306b |
| institution | Directory Open Access Journal |
| issn | 1868-7075 1868-7083 |
| language | English |
| last_indexed | 2024-04-14T07:50:31Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Clinical Epigenetics |
| spelling | doaj.art-15a234c6fca4438cac257f3838e8306b2022-12-22T02:05:11ZengBMCClinical Epigenetics1868-70751868-70832017-08-019111210.1186/s13148-017-0379-6Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transferHitoshi Hiura0Hiromitsu Hattori1Norio Kobayashi2Hiroaki Okae3Hatsune Chiba4Naoko Miyauchi5Akane Kitamura6Hiroyuki Kikuchi7Hiroaki Yoshida8Takahiro Arima9Department of Informative Genetics, Tohoku University Graduate School of MedicineDepartment of Informative Genetics, Tohoku University Graduate School of MedicineDepartment of Informative Genetics, Tohoku University Graduate School of MedicineDepartment of Informative Genetics, Tohoku University Graduate School of MedicineDepartment of Informative Genetics, Tohoku University Graduate School of MedicineDepartment of Informative Genetics, Tohoku University Graduate School of MedicineDepartment of Informative Genetics, Tohoku University Graduate School of MedicineCenter for Reproductive Medicine, Sendai ART ClinicCenter for Reproductive Medicine, Sendai ART ClinicDepartment of Informative Genetics, Tohoku University Graduate School of MedicineAbstract Background Frozen-thawed embryo transfer (FET) is increasingly available for the improvement of the success rate of assisted reproductive technologies other than fresh embryo transfer (ET). There have been numerous findings that FET provides better obstetric and perinatal outcomes. However, the birth weight of infants conceived using FET is heavier than that of those conceived via ET. In addition, some reports have suggested that FET is associated with perinatal diseases such as placenta accreta and pregnancy-induced hypertension (PIH). Results In this study, we compared the microRNA (miRNA) expression profiles in term placentae derived from FET, ET, and spontaneous pregnancy (SP). We identified four miRNAs, miR-130a-3p, miR-149-5p, miR-423-5p, and miR-487b-3p, that were significantly downregulated in FET placentae compared with those from SP and ET. We found that DNA methylation of MEG3-DMR, not but IG-DMR, was associated with miRNA expression of the DLK1-DIO3 imprinted domain in the human placenta. In functional analyses, GO terms and signaling pathways related to positive regulation of gene expression, growth, development, cell migration, and type II diabetes mellitus (T2DM) were enriched. Conclusions This study supports the hypothesis that the process of FET may increase exposure of epigenome to external influences.http://link.springer.com/article/10.1186/s13148-017-0379-6Assisted reproductive technologies (ART)Frozen-thawed embryo transfer (FET)PlacentaMicroRNAs (miRNAs)MicroarrayReal-time PCR |
| spellingShingle | Hitoshi Hiura Hiromitsu Hattori Norio Kobayashi Hiroaki Okae Hatsune Chiba Naoko Miyauchi Akane Kitamura Hiroyuki Kikuchi Hiroaki Yoshida Takahiro Arima Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer Clinical Epigenetics Assisted reproductive technologies (ART) Frozen-thawed embryo transfer (FET) Placenta MicroRNAs (miRNAs) Microarray Real-time PCR |
| title | Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
| title_full | Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
| title_fullStr | Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
| title_full_unstemmed | Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
| title_short | Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer |
| title_sort | genome wide microrna expression profiling in placentae from frozen thawed blastocyst transfer |
| topic | Assisted reproductive technologies (ART) Frozen-thawed embryo transfer (FET) Placenta MicroRNAs (miRNAs) Microarray Real-time PCR |
| url | http://link.springer.com/article/10.1186/s13148-017-0379-6 |
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