Human Brown Adipose Tissue Estimated With Magnetic Resonance Imaging Undergoes Changes in Composition After Cold Exposure: An in vivo MRI Study in Healthy Volunteers
Aim: Magnetic resonance imaging (MRI) is increasingly being used to evaluate brown adipose tissue (BAT) function. Reports on the extent and direction of cold-induced changes in MRI fat fraction and estimated BAT volume vary between studies. Here, we aimed to explore the effect of different fat fract...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fendo.2019.00898/full |
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| author | Gustavo Abreu-Vieira Aashley S. D. Sardjoe Mishre Aashley S. D. Sardjoe Mishre Jedrzej Burakiewicz Laura G. M. Janssen Kimberly J. Nahon Jari A. van der Eijk Titia T. Riem Mariëtte R. Boon Oleh Dzyubachyk Andrew G. Webb Patrick C. N. Rensen Hermien E. Kan |
| author_facet | Gustavo Abreu-Vieira Aashley S. D. Sardjoe Mishre Aashley S. D. Sardjoe Mishre Jedrzej Burakiewicz Laura G. M. Janssen Kimberly J. Nahon Jari A. van der Eijk Titia T. Riem Mariëtte R. Boon Oleh Dzyubachyk Andrew G. Webb Patrick C. N. Rensen Hermien E. Kan |
| author_sort | Gustavo Abreu-Vieira |
| collection | DOAJ |
| description | Aim: Magnetic resonance imaging (MRI) is increasingly being used to evaluate brown adipose tissue (BAT) function. Reports on the extent and direction of cold-induced changes in MRI fat fraction and estimated BAT volume vary between studies. Here, we aimed to explore the effect of different fat fraction threshold ranges on outcomes measured by MRI. Moreover, we aimed to investigate the effect of cold exposure on estimated BAT mass and energy content.Methods: The effects of cold exposure at different fat fraction thresholding levels were analyzed in the supraclavicular adipose depot of nine adult males. MRI data were reconstructed, co-registered and analyzed in two ways. First, we analyzed cold-induced changes in fat fraction, T2* relaxation time, volume, mass, and energy of the entire supraclavicular adipose depot at different fat fraction threshold levels. As a control, we assessed fat fraction differences of deltoid subcutaneous adipose tissue (SAT). Second, a local analysis was performed to study changes in fat fraction and T2* on a voxel-level. Thermoneutral and post-cooling data were compared using paired-sample t-tests (p < 0.05).Results: Global analysis unveiled that the largest cold-induced change in fat fraction occurred within a thermoneutral fat fraction range of 30–100% (−3.5 ± 1.9%), without changing the estimated BAT volume. However, the largest cold-induced changes in estimated BAT volume were observed when applying a thermoneutral fat fraction range of 70–100% (−3.8 ± 2.6%). No changes were observed for the deltoid SAT fat fractions. Tissue energy content was reduced from 126 ± 33 to 121 ± 30 kcal, when using a 30–100% fat fraction range, and also depended on different fat fraction thresholds. Voxel-wise analysis showed that while cold exposure changed the fat fraction across nearly all thermoneutral fat fractions, decreases were most pronounced at high thermoneutral fat fractions.Conclusion: Cold-induced changes in fat fraction occurred over the entire range of thermoneutral fat fractions, and were especially found in lipid-rich regions of the supraclavicular adipose depot. Due to the variability in response between lipid-rich and lipid-poor regions, care should be taken when applying fat fraction thresholds for MRI BAT analysis. |
| first_indexed | 2024-12-11T05:52:02Z |
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| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-11T05:52:02Z |
| publishDate | 2020-01-01 |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-15a274d6c28646b1a78bc2da2a5f2bba2022-12-22T01:18:47ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922020-01-011010.3389/fendo.2019.00898503533Human Brown Adipose Tissue Estimated With Magnetic Resonance Imaging Undergoes Changes in Composition After Cold Exposure: An in vivo MRI Study in Healthy VolunteersGustavo Abreu-Vieira0Aashley S. D. Sardjoe Mishre1Aashley S. D. Sardjoe Mishre2Jedrzej Burakiewicz3Laura G. M. Janssen4Kimberly J. Nahon5Jari A. van der Eijk6Titia T. Riem7Mariëtte R. Boon8Oleh Dzyubachyk9Andrew G. Webb10Patrick C. N. Rensen11Hermien E. Kan12Division of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Department of Medicine, Leiden University Medical Center, Leiden, NetherlandsDivision of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Department of Medicine, Leiden University Medical Center, Leiden, NetherlandsDepartment of Radiology, C.J. Gorter Center for High Field MRI, Leiden University Medical Center, Leiden, NetherlandsDepartment of Radiology, C.J. Gorter Center for High Field MRI, Leiden University Medical Center, Leiden, NetherlandsDivision of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Department of Medicine, Leiden University Medical Center, Leiden, NetherlandsDivision of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Department of Medicine, Leiden University Medical Center, Leiden, NetherlandsDepartment of Radiology, C.J. Gorter Center for High Field MRI, Leiden University Medical Center, Leiden, NetherlandsDivision of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Department of Medicine, Leiden University Medical Center, Leiden, NetherlandsDivision of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Department of Medicine, Leiden University Medical Center, Leiden, NetherlandsDivision of Image Processing (LKEB), Department of Radiology, Leiden University Medical Center, Leiden, NetherlandsDepartment of Radiology, C.J. Gorter Center for High Field MRI, Leiden University Medical Center, Leiden, NetherlandsDivision of Endocrinology and Einthoven Laboratory for Experimental Vascular Medicine, Department of Medicine, Leiden University Medical Center, Leiden, NetherlandsDepartment of Radiology, C.J. Gorter Center for High Field MRI, Leiden University Medical Center, Leiden, NetherlandsAim: Magnetic resonance imaging (MRI) is increasingly being used to evaluate brown adipose tissue (BAT) function. Reports on the extent and direction of cold-induced changes in MRI fat fraction and estimated BAT volume vary between studies. Here, we aimed to explore the effect of different fat fraction threshold ranges on outcomes measured by MRI. Moreover, we aimed to investigate the effect of cold exposure on estimated BAT mass and energy content.Methods: The effects of cold exposure at different fat fraction thresholding levels were analyzed in the supraclavicular adipose depot of nine adult males. MRI data were reconstructed, co-registered and analyzed in two ways. First, we analyzed cold-induced changes in fat fraction, T2* relaxation time, volume, mass, and energy of the entire supraclavicular adipose depot at different fat fraction threshold levels. As a control, we assessed fat fraction differences of deltoid subcutaneous adipose tissue (SAT). Second, a local analysis was performed to study changes in fat fraction and T2* on a voxel-level. Thermoneutral and post-cooling data were compared using paired-sample t-tests (p < 0.05).Results: Global analysis unveiled that the largest cold-induced change in fat fraction occurred within a thermoneutral fat fraction range of 30–100% (−3.5 ± 1.9%), without changing the estimated BAT volume. However, the largest cold-induced changes in estimated BAT volume were observed when applying a thermoneutral fat fraction range of 70–100% (−3.8 ± 2.6%). No changes were observed for the deltoid SAT fat fractions. Tissue energy content was reduced from 126 ± 33 to 121 ± 30 kcal, when using a 30–100% fat fraction range, and also depended on different fat fraction thresholds. Voxel-wise analysis showed that while cold exposure changed the fat fraction across nearly all thermoneutral fat fractions, decreases were most pronounced at high thermoneutral fat fractions.Conclusion: Cold-induced changes in fat fraction occurred over the entire range of thermoneutral fat fractions, and were especially found in lipid-rich regions of the supraclavicular adipose depot. Due to the variability in response between lipid-rich and lipid-poor regions, care should be taken when applying fat fraction thresholds for MRI BAT analysis.https://www.frontiersin.org/article/10.3389/fendo.2019.00898/fullbrown adipose tissuelipid metabolismcold exposurethermogenesismagnetic resonance imagingfat fraction |
| spellingShingle | Gustavo Abreu-Vieira Aashley S. D. Sardjoe Mishre Aashley S. D. Sardjoe Mishre Jedrzej Burakiewicz Laura G. M. Janssen Kimberly J. Nahon Jari A. van der Eijk Titia T. Riem Mariëtte R. Boon Oleh Dzyubachyk Andrew G. Webb Patrick C. N. Rensen Hermien E. Kan Human Brown Adipose Tissue Estimated With Magnetic Resonance Imaging Undergoes Changes in Composition After Cold Exposure: An in vivo MRI Study in Healthy Volunteers Frontiers in Endocrinology brown adipose tissue lipid metabolism cold exposure thermogenesis magnetic resonance imaging fat fraction |
| title | Human Brown Adipose Tissue Estimated With Magnetic Resonance Imaging Undergoes Changes in Composition After Cold Exposure: An in vivo MRI Study in Healthy Volunteers |
| title_full | Human Brown Adipose Tissue Estimated With Magnetic Resonance Imaging Undergoes Changes in Composition After Cold Exposure: An in vivo MRI Study in Healthy Volunteers |
| title_fullStr | Human Brown Adipose Tissue Estimated With Magnetic Resonance Imaging Undergoes Changes in Composition After Cold Exposure: An in vivo MRI Study in Healthy Volunteers |
| title_full_unstemmed | Human Brown Adipose Tissue Estimated With Magnetic Resonance Imaging Undergoes Changes in Composition After Cold Exposure: An in vivo MRI Study in Healthy Volunteers |
| title_short | Human Brown Adipose Tissue Estimated With Magnetic Resonance Imaging Undergoes Changes in Composition After Cold Exposure: An in vivo MRI Study in Healthy Volunteers |
| title_sort | human brown adipose tissue estimated with magnetic resonance imaging undergoes changes in composition after cold exposure an in vivo mri study in healthy volunteers |
| topic | brown adipose tissue lipid metabolism cold exposure thermogenesis magnetic resonance imaging fat fraction |
| url | https://www.frontiersin.org/article/10.3389/fendo.2019.00898/full |
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